ABSTRACT
Gerd is relatively uncommon in developing countries but H. pylori known to be the most common important cause of gastritis and associated with duodenal and gastric ulcers. The aim of our study was to find the relationship of H . pylori infection and GERD in Egyptian patients. The patients were selected and divided into two groups. Group I included 40 patients suffering from GERD [19 males and 21 females] and their ages ranged from [24 to 70] years; Group II, included 20 non GERD patients, with ulcerative or non ulcerative dyspepsia with H . pylori positive [12 males 8 females] and their ages ranged from [20] to [55] years. All patients were subjected to full history taking; clinical examination, laboratory investigation, endoscopic examination and three biopsies were taken from antrum of stomach to recognize H . pylori by R.U.T. and histopathological examination
Results of the present study showed that no significant positive association between H. pylori and pathogenesis of GERD where H. pylori positive with GERD occur in five patients [12.5%] especially in grade I reflux eosophagitis and H. pylori negative with GERD occur in [28] patients [70%]. There were seven patients [17.5%] with incompetent cardia and hiatus hernia hadpositive H. pylori without reflux eosophagitis. We conclude neither symptoms of GERD nor endoscopic grading of reflux eosophagitis has no relation with H. pylori infection
ABSTRACT
Humoral IgM, IgG and mucosal IgA response to recmbinant cystein rich portion [designated LC3] of the entamoeba histolytica galactose inhibitable adherence lectin 170KDa subunite were determined in pateints with acute diarrhea. One hundred subjects presented with acute diarrhea subdivided into two groups; 46 pateints with positive foecal GIAP antigen group la and 54 with negative foecal GIAP antigen group Ib. We found that the pateints with amoebic diarrhea group la had anti-LC3 IgM and IgG 68.75%; 69.2% respectively compared with zero percent* in non-invasive amaeba [E. dispar] infestation. Serum anti-LC3 IgG antibodies can differentiate subjects with invasive amoebiasis after one-week duration of illness from those with E. dispar and controls [9.9%]. However, the long life span of IgG led to false positive results especially in endemic areas like Egypt. In conclusion, the early detection of anti-LC3 IgM antibodies is helpful in diagnosis of recent E. histolytica infection
ABSTRACT
Recombinant LC3-encoded protein is highly antigenic, immunogenic and protective in gerbil model of amebic liver abscess as subunit vaccine with titermax as an adjuvant. The aim of this work is to study the LC-3 incoded protein as a subunit vaccine. To reach our goal two groups of BALB/c mice had immunized with LC3-encoded protein and cholera toxin [CT] as an adjuvant. Group I immunized with l.0Mg of LC3 with 10 ug of cholera toxin group II with 10 ug of cholera toxin only. We found that secretory IgA, serum IgA and serum IgG were siginificantly increased in group I compared with group II [p<0.0001]. In conclusion, oral immunization of BALB/c mice with recombinant LC3-encoded lectin with CT as an adjuvant was effective in inducing intestinal and systemic IgA and IgG