ABSTRACT
Cervical cancer is a kind of malignant tumor that seriously endangers women's health. Cervical cancer has a clear etiology and easy sampling, which can be early detected by screening, and its development can be blocked by early intervention. Cervical cancer screening has been launched for rural women since 2009 in China in order to reduce the incidence of cervical cancer. However, the incidence of cervical cancer has not significantly reduced after ten years. There are three common pain points in the current cervical cancer screening program, including inaccurate sampling, invalid management and no integrated technology. The resolution of these problems will promote the development of cervical cancer screening and the establishment of the long-term mechanism of the prevention and treatment system in China. On the basis of previous studies and in combination with our innovative technologies, we put the observation function of colposcope on the first step for the screening of cervical cancer, which not only ensures accurate sampling, but also further integration of VIA/VILI, HPV typing, liquid-based cytology (TCT) and p16/ki-67 to improve the detection rate of cervical cancer and reduce false negatives. Those women in high-risk groups found by screening can be automatically followed up and given medical advices in the intelligent medical and health management platform for cervical cancer screening. Through repeatedly discussion by expert committee, we put forward expert consensus (2019) for early cervical cancer screening and abnormal crowd management in China. This expert consensus can help doctors in scientific research, clinical practice and the establishment of national policy of cervical cancer screening. We will continuously provide more data to improve cervical cancer screening process in the future.
ABSTRACT
Objective There are few large sample data reports of comparative study on genotype distribution of human papillomavirus (HPV) infection in the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma in China. This study aimed to investigate the clinical value of genotype distribution of HPV infection in the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma in regional (mainly in Jiangsu Province) patients.Methods We collected 1044 paraffin tissue specimens of female cervical squamous cell carcinoma (826 cases) and cervical adenocarcinoma (218 cases) in 29 hospitals from November 1978 to December 2017. HPV DNA was extracted from the tissues and through the combination of gene-chip and polymerase chain reaction technology, 23 genotypes of HPV were detected in all the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma, and comparative study was conducted on the genotype distribution of HPV infection.Results Among 1044 cases of cervical squamous cell carcinoma and cervical adenocarcinoma, 901 were found with HPV and the detection rate was 86.30%. The detection rate of cervical squamous cell carcinoma was 91.53% (756/826), among which 16，18，58，33，52，31 types were the most common and the detection rate of 16 type was significantly higher than that of 18 types (56.84% vs 9.93%, P0.05).Conclusion The HPV detection rates are different in the regional female cervical squamous cell carcinoma and cervical adenocarcinoma tissues. 16,18,31,33,52 and 58 types are the most common genotypes in cervical squamous cell carcinoma and cervical adenocarcinoma. The detection rate of 16 type is overly higher than that of 18 type in cervical squamous cell carcinoma, while the detection rates of 16 type and 18 type in cervical adenocarcinoma are very close.
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the infection condition of human papillomavirus (HPV) type 16 and 18 in the squamous cells and columnar cells of patients with common anorecatal lesions.</p><p><b>METHODS</b>Infections of HPV type 16 and 18 were determined with real-time fluorescent quantitative PCR in the wax-embedded surgical specimen of 805 patients with common anorectal diseases.</p><p><b>RESULTS</b>The overall infection rate among 805 patients with anorecatal lesions was 66.1% (532/805). The infection rate was 82.6% (95/115) in patients with mixed hemorrhoids, 76.5% (88/115) in anal papillary fibromas, 74.8% (86/115) in internal hemorrhoids, 72.2% (83/115) in fistulas, 69.6% (80/115) in external hemorrhoids, 47.8% (55/115) in anal perianal abscesses, and 39.1% (45/115) in anal fissures.</p><p><b>CONCLUSION</b>Infection rate of HPV type 16, 18 in common anorectal lesions is high.</p>
Subject(s)
Humans , Human papillomavirus 16 , Human papillomavirus 18 , Papillomavirus Infections , Rectal Diseases , EpidemiologyABSTRACT
<p><b>OBJECTIVE</b>To study the value of screening hereditary nonpolyposis colorectal cancer (HNPCC) kindreds by detecting the expressions of hMLH1/hMSH2 with tissue microarray.</p><p><b>METHODS</b>A tissue microarray with 22 colorectal cancers from HNPCC families and 15 sporadic colorectal cancers was established, and the expressions of hMLH1/hMSH2 were detected by immunohistochemistry (IHC).</p><p><b>RESULTS</b>The expressions of hMLH1 or hMSH2 were negative in 15 of 22 HNPCC and 1 of 15 sporadic colorectal cancers in routine IHC. The expressions of hMLH1 or hMSH2 were negative in 17 of 22 HNPCC and 2 of 15 sporadic colorectal cancers in tissue microarray. The examination of hMSH2 expression yielded same results between routine IHC and tissue microarray. There were no difference on the hMLH1 expressions between routine IHC and tissue microarray.</p><p><b>CONCLUSION</b>Tissue microarray is a high-throughput way to detect the expressions of hMLH1/hMSH2 and is applicable to screen HNPCC kindreds.</p>