ABSTRACT
BACKGROUND: It is commonly believed that hyperbaric oxygen (HBO)plays a neuroprotective role in the prevention and treatment of cerebral infarction (CI), however hyperbaric oxygen therapy (HBOT)-induced CI was also reported.OBJECTIVE: To investigate the risk factors of HBOT-induced CI.DESIGN: Case analysis with patients as subjects.SETTING: At the Department of Hyperbaric Oxygen of Guangzhou General Hospital of Guangzhou Military Command Area of Chinese PLA.PARTICIPANTS: Totally 192 inpatients receiving HBOT were selected from the Department of Hyperbaric Oxygen of Guangzhou General Hospital Guangzhou Military Command Area of Chinese PLA between December 1996 and March 1998, including 127 males and 65 females with age ranging from 9 to 78 years old. Totally 6 patients, 3 males and 3 females, suffered from CI during HBOT, with the age from 51 to 76 yeas.METHODS: The background factors of 192 patients who HBOT METHODS and 6 cases with induced CI were analyzed.MAIN OUTCOME MEASURES: Symptoms, signs and skull CT or MRI.RESULTS: Data of 192 patients were dealt with intentionally and all were statistically analyzed without miss. ① The incidence of CI during HBOT was 0.3% (6/192). ② The risk factors of HBOT-induced CI included: age of ≥ 60 years, hypertension, hyperlipidemia, primary CI or cerebral hemorrhage, blood hyperviscosity and diabetes, and the incidence would greatly increased in those who has 4 or more than 4 risk factors, but seldom with less than 4 risk factors.CONCLUSION: The cluster level of risk factors was closely related with the incidence of HBOT-induced CI.
ABSTRACT
Objective:To enhance the immune efficiency of porcine reproductive and respiratory syndrome virus(PRRSV)DNA vaccine,the ORF5 genes were used as candidate genes to construct the recombinant plasmids CpG-pVAX1-ORF5 by pVAX1 as eukaryotic expression vector.The aim is to analyze the immune responses and the effects of CpG-ODN induced by GP5 recombinant plasmids of PRRSV.Methods:Piglets were immunized with recombinant DNA plasmids which expressed PRRSV GP5 for three inoculations.The PRRSV antibody in serum,the concentration of IL-2 and the lymphocyte proliferation test(MTT) in peripheral blood of vaccinated piglets were detected.The vaccinated pigs were challenged intranasally with PRRSV SD2.Results:GP5 DNA immunization with CpG resulted in the production of both PRRSV antibodies and cellular immune(a significant enhancement of a lymphoproliferative response ).The CpG-pVAX1-ORF5 was showed significantly better protection from the PRRSV challenge compared with the control plasmid.This immune response was characterized by a significantly decreased frequency of viraemia with decrease of 80% compared with the control group.There were little clinical symptoms and protection in some extent from lung damage.Conclusion:The results indicate that CpG-ODN could be used as immune adjuvant of PRRSV DNA vaccine.