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Objective:To explore the mechanism of Xiaojinwan in treating breast cancer bone metastases through cell experiments and bioinformatic analysis. Method:The inhibitory effect of Xiaojinwan on MCF-7 cell viability was detected by cell counting kit-8 （CCK-8） assay. The key components and targets responsible for Xiaojinwan in inhibiting breast cancer bone metastases were predicted by network pharmacology and molecular docking. The active components and targets of Xiaojinwan were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCSMP） and SwissTarget Prediction， and the breast cancer bone metastases-related targets from GeneCards and DisGeNET. The results were imported into STRING for constructing a protein-protein interaction （PPI） network， followed by Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis using DAVID. A network of the active components of Xiaojinwan-breast cancer bone metastases-related targets-pathways was constructed using Cytoscape 3.7.2. AutoDock 4 was employed for molecular docking. The protein expression levels of matrix metallopmteinase-9 （MMP-9）， hypoxia-inducible factor 1<italic>α </italic>（HIF1A）， and androgen receptor （AR） were assayed by Western blot. Result:Xiaojinwan inhibited the viability of MCF-7 cells and acted on breast cancer bone metastases through such processes as redox and protein autophosphorylation. KEGG enrichment analysis showed that HIF-1， vascular endothelial growth factor （VEGF） and phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） pathways were involved. As verified by molecular docking， the active components such as eucalyptin stably bound to AR and MMP-9. Western blot indicated that Xiaojinwan dose-dependently inhibited the expression of MMP-9 and HIF1A proteins in MCF-7 cells. Conclusion:Xiaojinwan acts on AR and MMP-9 through HIF， VEGF and other related signaling pathways， thereby improving hypoxia in tumor microenvironment， inhibiting angiogenesis， and reducing cell invasion and viability.
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Objective:To explore the possible mechanism of Yanghe Huayantang in reversing the drug resistance of breast cancer by observing the effect of Yanghe Huayantang on the transplant tumor of tamoxifen （TAM）-resistant breast cancer and its influences on the interaction pathway of estrogen receptor （ER）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt）/mammalian rapamycin target protein （mTOR）. Method:Fifty mice were randomly divided into 5 groups： blank group， model group， Yanghe Huayantang group， everolimus group， and Yanghe Huayantang+everolimus group. The model of kidney deficiency was established by bilateral ovariectomy， and the blank group was treated with sham operation. Three days after the establishment of the model， all the five groups of mice were inoculated with breast cancer TAM drug-resistant cells （MCF-7/TAM<sup>-</sup>） to establish breast cancer TAM -resistant transplanted tumor model. After successful modeling， Yanghe Huayantang group received intragastric administration of Yanghe Huayantang （traditional Chinese medicine preparation 20 mL·kg<sup>-1</sup>）， everolimus group received intraperitoneal injection of everolimus （10 mg·kg<sup>-1</sup>）. Yanghe Huayantang + everolimus group received Yanghe Huayantang by intragastric administration and everolimus by intraperitoneal injection. The blank group and model group received intragastric administration and intraperitoneal injection of phosphate buffer （PBS）. Drug administration was lasted for 28 days in all groups， once a day. After administration， the tumor tissue was separated and weighed， and the tumor inhibition rate was calculated. Hematoxylin-eosin （HE） staining was used to observe the pathological changes of tumor tissue. Immunofluorescence and Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） were used to detect the expression of PI3K， Akt， mTOR， ER protein and mRNA in tumor tissue. Result:Compared with the model group， the tumor volume and tumor weight of Yanghe Huayantang group decreased significantly on the 12th， 20th and 28th days （<italic>P</italic><0.01）， and the tumor inhibition rate increased significantly （<italic>P</italic><0.01）.Yanghe Huayantang group significantly reduced the density of tumor cells and caused tumor cell necrosis. Compared with the model group， Yanghe Huayantang group， everolimus group and Yanghe Huayantang+everolimus group inhibited the expression of PI3K， Akt， mTOR protein and mRNA （<italic>P</italic><0.05， <italic>P</italic><0.01）. Compared with the blank group， Yanghe Huayantang group， everolimus group and Yanghe Huayantang+everolimus group all inhibited the protein and mRNA expression of ER， and mRNA expression of ER in Yanghe Huayantang+everolimus group was significantly lower than that in the model group （<italic>P</italic><0.01）. Conclusion:Yanghe Huayantang can inhibit the growth of TAM-resistant breast cancer. The mechanism may be that Yanghe Huayantang can reverse the TAM resistance of breast cancer by down-regulating the expression of key molecules of ER/PI3K/Akt/mTOR cross-signal pathway.
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Objective To observe the sealing effect of transient pinus massoniana bark extract (PMBE)pre-coating on dentin tubules and its wear resistance so as to provide an experimental basis for PMBE to be used to prevent and treat dentin hypersensitivity.Methods The model of dentin hypersensitivity was established by acid etching method.The samples were transient pre-coating with 80 g/L of PMBE ethanol solution,and then some of them experienced brushing wear treatment with ethanol group and fluorine vanish group as negative and positive controls.The surface and profile morphologies of the samples were observed using field emission scanning electron microscope (FESEM)in each group.The area of dentin tubular openings (ADTO)and percentage area of dentin tubular openings (PADTO)were also measured.Results The dentin tubules were opened completely and the lumen of dentin tubules was empty in ethanol group.The dentin tubules were completely or basically closed and some films or plugging could be seen in the lumen of dentin tubules in fluorine vanish/immediate group and PMBE/immediate group.The dentin tubules were partly opened and some wear marks could be seen on the dentin surface in fluorine vanish/wear groups,PMBE/wear groups.ADTO and PADTO in ethanol group were significantly higher than those in fluorine vanish groups and PMBE groups (P＜0.05).ADTO and PADTO did not significantly differ between fluorine vanish/wear groups and PMBE/wear groups (P＞0.05),which were all significantly higher than those in fluorine vanish/immediate group and PMBE/immediate group (P＜0.05).Conclusion Local transient pre-coating of PMBE ethanol solution can achieve fine immediate sealing effect of dentin tubules and some degree of wear resistance.
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· Ocular surface squamous neoplasia(OSSN) is a type of ocular surface tumor,which is difficult to diagnose because of its low incidence is low and it is easy to be confused with pterygium.In recent years,the improvement of various diagnostic techniques and the extensive application of interferon (IFN) to OSSN therapy have greatly reduced the recurrence rate,and the diagnostic and therapeutic methods of OSSN tend to be diversified.Understanding the current causes of OSSN and the ways of treatment can better guide the OSSN study.
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AIM:To investigate the effect of intense pulsed light on the meibomian gland dysfunction ( MGD) , and to observe whether the intense pulsed light can improve the symptoms and objective indexes of the patients with meibomian gland dysfunction. · METHODS: A retrospective noncomparative interventional case series was conducted. Totally 21 MGD patients (42 eyes) were selected from November 2016 to February 2017 in Renmin Hospital of Wuhan University, the results of the following tests is recorded:OSDI score, corneal fluorescein staining, tear break-up time, ocular surface analyzer, scanning confocal microscopy, all patients respectively were received intense pulsed light treatment 3 times at 3wk intervals, and followed up after 1mo. Paired sample t test was used to analyze the difference in outcome. ·RESULTS: In the 21 cases ( 42 eyes ) , the OSDI score decreased, the mean value before treatment was 30. 18 ± 4. 07, and the mean value after treatment was 24. 87±4. 32. The first tear film break-up time ( first, BUT) increased, the mean value before treatment was 5. 37 ± 0. 82s, the mean value after treatment was 7. 12 ± 0. 74s. The mean value of meibomian gland secretion scores before the treatment was 1. 57 ± 0. 52,the mean value after treatment was 1. 22 ± 0. 52. The mean value of corneal fluorescence staining before treatment was 0. 82 ± 0. 41, and the mean value after treatment was 0. 51 ± 0. 53, the difference was statistically significant (t=11. 2, 2. 68, 3. 31, 2. 78, 2. 61;P<0. 05). · CONCLUSION: Intense pulsed light can effectively improve the subjective symptoms of patients with MGD and alleviate the obstruction of meibomian glands. It is an important method for MGD treatment.
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<p><b>OBJECTIVE</b>To explore the effect of Yanghe Huayan Decoction (YHD, a representative recipe for warming yang mass dissipating) in inhibiting precancerosis of breast cancer (PBC) and on the protein and mRNA expression of ki67.</p><p><b>METHODS</b>The PBC rat model was established by dimethylbenzanthracene (DMBA), and 9 weeks later rats were randomly divided into the blank control group, the model group, the YHD group, the Sanjie Huatan Decoction group (SHD), the Pingxiao Tablet group (PT), and the tamoxifen group. Rats in the model group were administered with water by gastrogavage. Rats in the YHD group received YHD (deglued antler powder 12 g, prepared rhizome of rehmannia 9 g, cassia bark 6 g, white mustard seed 3 g, zedoary root 12 g, appendiculate cremastra pseudobulb 15 g, chekiang fritillary bulb 9 g, licorice root 6 g) at the daily dose of 7.2 g/kg by gastrogavage. Rats in the SHD group received SHD (oldenlandia diffusa 15 g, Scutellaria Barbata 15 g, Trichosanthes Kirilowii 15 g, pinellia 9 g) at the daily dose of 5.4 g/kg by gastrogavage. Rats in the PT group received PT at the daily dose of 144 mg/kg by gastrogavage. Those in the tamoxifen group received tamoxifen at the daily dose of 4 mg/kg by gastrogavage. Pathomorphological changes of the breast tissue were observed by HE staining. The positive rate and the gray value of ki67 expression were detected by immunohistochemical assay. And the expression of ki67 mRNA was detected by q-PCR.</p><p><b>RESULTS</b>Compared with the model group, the general hyperplasia and the occurrence rate of precancerous lesion were higher and the occurrence rate of invasive carcinoma was lower in each treatment group (P < 0.05). Except the SHD group, the intensity of ki67 grey value increased in each treatment group (P < 0.05, P < 0.01). Except the PT group, the positive rate of ki67 and mRNA expression of ki67 increased in the rest treatment groups (P < 0.05, P < 0.01). Compared with the YHD group, there was no statistical difference in the occurrence rate of infiltration or the occurrence rate of precancerous lesion (P > 0.05). The positive rate of ki67 expression and mRNA expression of ki67 increased in the PT group, showing statistical difference (P < 0.05).</p><p><b>CONCLUSIONS</b>YHD could partially inhibit and reverse canceration of breast cancer. It also could inhibit ki67 protein and mRNA expression. Its effect was similar to tamoxifen and superior to PT. So it was suitable for prevention and treatment of precancerous lesion of breast cancer.</p>
Subject(s)
Animals , Female , Rats , Disease Models, Animal , Drugs, Chinese Herbal , Therapeutic Uses , Ki-67 Antigen , Metabolism , Mammary Neoplasms, Experimental , Drug Therapy , Metabolism , Precancerous Conditions , Drug Therapy , Metabolism , Rats, Sprague-DawleyABSTRACT
To observe the effect of various doses of oil of Piper longum unsaponifiable matter (OPUM) to cholesterol gallstones in experimental mice. C57BL/6 mice (n = 60) were randomly divided into 6 groups: control group, model group, OPUM (15, 30 and 60 mg x kg(-1)) group and ursodeoxycholic acid (UDCA, 60 mg x kg(-1)) group, administered for 10 weeks. The level of serum lipid and liver function enzymes were tested. The gallbladder was removed and bile was obtained by centrifugation. Next, the levels of the bile total cholesterol (TC), phospholipid (PL) and bile acid (TBA) were measured. The indicators of lipid peroxidation were determined and cholesterol saturation index (CSI) was calculated. The liver histological changes were observed by HE staining. The results showed that serum TC, TG (triglycerides) and AST (aspartate transaminase) contents, gallbladder cholesterol crystallization and CSI increased significantly (P < 0.05). In addition, the activity of SOD decreased significantly and MDA content increased significantly in liver (P < 0.05). HE staining results showed that the hepatic cord disorder and intracellular lipid droplets increased significantly. All results indicate that lithogenic diet lead to the formation of cholesterol gallstones. In OPUM (30 and 60 mg x kg(-1)) group, serum TC, TG and AST content, gallbladder cholesterol crystallization and CSI decreased significantly, the activity of SOD increased significantly and MDA content decreased significantly. HE staining results showed that OPUM can improve the morphology of liver cell, reduce the degree of hepatic cord disorders and restore the cell morphology close to normal. The cause of OPUM prevents cholesterol gallstone formation maybe due to protect the integrity of the liver cells, lower CSI, and reduce cholesterol crystal formation and hence prevent cholesterol gallstone formation.
Subject(s)
Animals , Male , Mice , Aspartate Aminotransferases , Blood , Bile , Chemistry , Cholesterol , Blood , Gallbladder , Gallstones , Metabolism , Liver , Metabolism , Pathology , Malondialdehyde , Metabolism , Mice, Inbred C57BL , Piper , Chemistry , Plant Oils , Pharmacology , Plants, Medicinal , Chemistry , Random Allocation , Superoxide Dismutase , Metabolism , Triglycerides , BloodABSTRACT
<p><b>OBJECTIVE</b>To evaluate the application of health assessment instruments in Chinese medicine.</p><p><b>METHODS</b>According to a pre-defined search strategy, a comprehensive literature search for all articles published in China National Knowledge Infrastructure databases was conducted. The resulting articles that met the defined inclusion and exclusion criteria were used for analysis.</p><p><b>RESULTS</b>A total of 97 instruments for health outcome assessment in Chinese medicine have been used in fundamental and theoretical research, and 14 of these were also used in 29 clinical trials that were randomized controlled trials, or descriptive or cross-sectional studies. In 2 152 Chinese medicine-based studies that used instruments in their methodology, more than 150 questionnaires were identified. Among the identified questionnaires, 51 were used in more than 10 articles (0.5%). Most of these instruments were developed in Western countries and few studies (4%) used the instrument as the primary evidence for their conclusions.</p><p><b>CONCLUSION</b>Usage of instruments for health outcome assessment in Chinese medicine is increasing rapidly; however, current limitations include selection rationale, result interpretation and standardization, which must be addressed accordingly.</p>
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Humans , Databases, Factual , Medicine, Chinese Traditional , Outcome Assessment, Health Care , Methods , Randomized Controlled Trials as Topic , Research DesignABSTRACT
Objective To investigate the relation between cerebral infarction and both diabetes mellitus and carotid artery plaque. Methods Two hunderd and twenty-six patients with ischemic cerebral vascular diseases were chosen in our study; MRI was employed to determine whether new infarction existed or not; carotid artery plaque positive group and negative group were divided according to the carotid artery intima-media thickness(IMT) examined by carotid ultrasound; the gender,age and biochemical markers of these patients,and their coexistence of hypertension and diabetes mellitus were recorded.The positive rates of cerebral infarction,carotid artery plaque and diabetes mellitus were noted and analyzed by binary comparison, and the level-risk relation were analyzed by logistic regression.Results The positive rate of carotid artery plaque in patients with cerebral infarction (84％) was significantly higher as compared with that in patients without cerebral infarction (52％),and the positive rate of cerebral infarction in patients with carotid artery plaque (66％) was obviously higher as compared with that in patients without carotid artery plaque (28％); correlation analysis between cerebral infarction and carotid artery plaque through logistic regression showed that the OR value and 95％CI were 5.019and (2.694-9.354) before adjustment,and 4.459 and (2.061-9.647) after adjustment (P＜0.05).The positive rate of diabetes mellitus in patients with carotid artery plaque (38％) was significantly higher as compared with that in patients without carotid artery plaque (13％),and the positive rate of carotid artery plaque in patients with diabetes mellitus (87％) was obviously higher as compared with that in patients without diabetes mellitus (62％) ; correlation analysis between diabetes mellitus and carotid artery plaque through logistic regression showed that the OR value and 95％CI were 4.010 and (1.854-8.672) before adjustment,and 4.272 and (1.565-11.662) after adjustment (P＜0.05).The positive rate of diabetes mellitus in patients with cerebral infarction (38％) was significantly higher as compared with that in patients without cerebral infarction (21％),and the positive rate of cerebral infarction in patients with diabetes mellitus (68％) was significantly higher as compared with that in patients without diabetes mellitus (48％,P＜0.05); correlation analysis between diabetes mellitus and cerebral infarction through logistic regression showed that the OR value and 95％ CI were 2.256 and (1.242-4.095) before adjustment,and 2.256 and (1.242-4.095) after adjustment; when the carotid artery plaque was added in procedure of adjustment (P＜0.05),the results of OR value and 95％CI were 1.696 and (0.833-3.452),and no significant difference in the positive rate of cerebral infarction was noted between patients with diabetes mellitus and cerebral infarction (P＞0.05). Conclusion Diabetes mellitus contributes to cerebral infarction by promoting carotid artery plaque formation.
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Prevention and treatment of mammary cancer has been taken into great account recently. "Mutistage developing mode" provides the basis for interrupting and reversing precancerous changes. Traditional Chinese medicine (TCM) has shown effects on precancerous changes through inhibiting angiogenesis, promoting apoptosis, modulating endocrine system and restraining oncogene expression. It was stressed in this review that TCM should study the precancerous change of mammary cancer from the aspects of recognizing the essence of precancerous changes of mammary cancer, formulating the standard of TCM diagnosis and treatment, widening the aim of treatment, and focusing on the mechanism of Chinese herbal medicine in intervening it.
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Animals , Female , Humans , Angiogenesis Inhibitors , Therapeutic Uses , Apoptosis , Breast Neoplasms , Diagnosis , Drug Therapy , Diagnosis, Differential , Drugs, Chinese Herbal , Therapeutic Uses , Medicine, Chinese Traditional , Phytotherapy , Precancerous Conditions , Diagnosis , Drug TherapyABSTRACT
Integrative traditional Chinese and Western medicine (integrative medicine) is the advanced scientific thinking system in order to settle the relationship between traditional Chinese medicine and Western medicine, which is advocated based on the convergence of traditional Chinese and Western medicine and the scientifization of traditional Chinese medicine. The establishment of the guideline of integrative medicine and the unceasing acquirement of researches are one of the light spots of Chinese medical history in the 20th century. The medical pattern of integrative medicine founded gradually in uneven pathway in the past half century reveals its tremendous advantages increasingly and should give an important impact on the progress of the Chinese medicine and even that of the world medicine.