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Objective:To develop an online interactive cytopathological training program, and to evaluate it for improving the cytopathological diagnostic ability of endoscopists in endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) of pancreas.Methods:A total of 5 500 cytopathological images were collected from 194 patients with pancreatic solid mass who underwent EUS-FNA in Nanjing Drum Tower Hospital from August 2018 to August 2019. The cell type in each cytopathological picture was labeled by senior cellular pathologists, which was used to build a learning and testing platform for online interactive cytopathological training. Five endoscopists without cytopathological background were invited to participate in this training. Sensitivity, specificity, positive predictive value and negative predictive value of endoscopists in differential diagnosis of cancer and non-cancer before and after training were compared to evaluate the effect of the online interactive cytopathological training program on improving the ability of endoscopists in diagnosis of cytopathology.Results:A cytopathological training platform for endoscopists to learn and take online test was successfully built. Before training, sensitivity, specificity, positive predictive value, negative predictive value and accuracy of diagnosis of cancer and non-cancer for endoscopists were 0.55 (95% CI: 0.53-0.58), 0.32 (95% CI: 0.30-0.35), 0.43 (95% CI: 0.41-0.45), 0.44 (95% CI: 0.41-0.47) and 0.43 (95% CI: 0.42-0.45), respectively. After training, the above indicators were 0.96 (95% CI: 0.95-0.97), 0.70 (95% CI: 0.68-0.73), 0.74 (95% CI: 0.72-0.76), 0.95 (95% CI: 0.94-0.96) and 0.81 (95% CI: 0.80-0.83), respectively, which were significantly improved compared with those before ( P<0.001). Conclusion:The online interactive cytopathological training program can improve the understanding and diagnostic ability of endoscopists in pancreatic cytopathology, help to implement rapid on-site evaluation in the process of EUS-FNA, and improve the diagnostic efficiency of EUS-FNA.
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Objective To investigate the association of serum ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) level with severity,progression and prognosis of traumatic brain injury (TBI).Methods Sixty TBI patients,admitted to our hospital from March 2012 to June 2013,were chosen in our study,and divided into moderate TBI group (Glasgow coma scale [GCS] scores:9-12,n=30) and severe TBI group (GCS scores:3-8,n=30);30 healthy controls were chosen.The serum UCH-L1 levels 12 and 24 h,and 2,3 and 5 d after TBI were detected using enzyme-linked immunosorbent assay (ELISA).Additionally,the correlations between serum UCH-L1 level and both imaging data and prognosis of TBI were analyzed.Results As compared with that in the control group,the mean serum UCH-L1 level in moderate TBI group and severe TBI group 12 and 24 h,and 2 and 3 d after TBI was significantly increased (P＜0.05);as compared with that in the moderate TBI group,the serum UCH-L1 level in severe TBI group 12 and 24 h,and 2 and 3 d after TBI was significantly increased (P＜0.05).The serum UCH-L1 level in the moderate TBI group reached the peak at 12 h after TBI,then gradually declined and presented no significant difference 5 d after TBI in comparison with controls.In severe TBI group,serum UCH-L1 level reached the first peak at 12 h,then gradually decreased,and rose again for the second peak 3 d after TBI.Serum UCH-L1 level was closely related with imageological changes and negatively correlated with prognosis of TBI (r=-0.412,P=0.030).Conclusion Serum UCH-L1 level appears to have potential clinical utility in providing valuable information about severity,progression and prognosis of TBI.
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<p><b>OBJECTIVE</b>To study the safety and effectiveness of laparoscopic radiofrequency ablation for centrally located renal tumors.</p><p><b>METHODS</b>From January 2009 to April 2013, thirteen patients who diagnosed as centrally located renal tumors were treated with laparoscopic radiofrequency ablation in the Department of Urology of Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School. All of the cases were T1aN0M0 stage, 9 patients were male, 4 were female, the mean age was 56 years (range, 38-73 years). All tumors were unilateral, eight lesions were in the left kidney and five in the right kidney. Intraoperative real-time ultrasound and temperature probes were used to guide the range of radiofrequency ablation. Ice saline was injected through ureteral catheter for cooling the collecting system. The postoperative serum creatinine and glomerular filtration rate (GFR) data were collected,all patients were followed up with enhanced CT or MRI.The pre- and post-operative date were compared by paired t test.</p><p><b>RESULTS</b>All patients underwent laparoscopic radiofrequency ablation successfully. The mean operation time was (113±13) minutes and the mean blood loss was (99±23) ml. The mean pre- and post-operative serum creatinine was (71±11) µmol/L and (74±11) µmol/L, the mean pre- and post-operative GFR was (49±8) ml/min and (45±7) ml/min. There was no significant statistic difference between pre-operation and post-operation (t=-1.371 and 1.986, P>0.05). The mean follow-up was 37 months, range 12-63 months. No evidence of local recurrence or distant metastasis was found.</p><p><b>CONCLUSIONS</b>Laparoscopic radiofrequency ablation for T1aN0M0 centrally located renal tumors could be performed safely with good outcomes. Intraoperative real-time ultrasound and temperature probes are helpful to control the range of radiofrequency ablation. Physical cooling of renal collecting system could reduce the occurrence of postoperative hydronephrosis and leakage of urine.</p>