ABSTRACT
OBJECTIVES: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. MATERIALS AND METHODS: Thirteen New Zealand white male rabbits weighing 3.0+/-0.5 kg were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. RESULTS: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. CONCLUSION: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.
Subject(s)
Humans , Male , Rabbits , Animal Experimentation , Bone Density , Dental Implants , Femur , Low-Level Light Therapy , New Zealand , Osseointegration , Seoul , Survival Rate , Titanium , TorqueABSTRACT
Bilateral sagittal split ramus osteotomy is considered a standard technique in mandibular orthognathic surgeries to reduce unexpected bilateral stress in the temporomandibular joints. Unilateral sagittal split ramus osteotomy (USSO) was recently introduced to correct facial asymmetry caused by asymmetric mandibular prognathism and has shown favorable outcomes. If unilateral surgery could guarantee long-term postoperative stability as well as favorable results, operation time and the incidence of postoperative complications could be reduced compared to those in bilateral surgery. This report highlights three consecutive cases with long-term follow-up in which USSO was used to correct asymmetric mandibular prognathism. Long-term postoperative changes in the condylar contour and ramus and condylar head length were analyzed using routine radiography and computed tomography. In addition, prior USSO studies were reviewed to outline clear criteria for applying this technique. In conclusion, patients showing functional-type asymmetry with predicted unilateral mandibular movement of less than 7 mm can be considered suitable candidates for USSO-based correction of asymmetric mandibular prognathism with or without maxillary arch surgeries.
Subject(s)
Humans , Facial Asymmetry , Follow-Up Studies , Head , Incidence , Orthognathic Surgery , Osteotomy, Sagittal Split Ramus , Postoperative Complications , Prognathism , Radiography , Temporomandibular JointABSTRACT
0.05). Amnesia during local injection was observed in eight patients (34.8%). Compared with the preoperative anxiety score, the intraoperative anxiety score was decreased.CONCLUSION: In this study, we found cardiovascular and respiratory stability in intravenous sedation using dexmedetomidine with pethidine, in plate and screw removal, after orthognathic surgery. Furthemore, intravenous sedation using dexmedetomidine with pethidine shows adequate analgesic and sedative effects.
Subject(s)
Humans , Amnesia , Anxiety , Arterial Pressure , Blood Pressure , Dexmedetomidine , Heart Rate , Hypnotics and Sedatives , Meperidine , Orthognathic Surgery , OxygenABSTRACT
Subject(s)
Animals , Humans , Anesthesia, General , Azaperone , Durapatite , Osteoblasts , Osteogenesis , Polyethylenes , Polypropylenes , Seeds , SwineABSTRACT
0.05).CONCLUSION: The angle between the Lo line and IP line (angle of the Lo-IP line) showed no statistically significant difference in both the control and asymmetry groups. Therefore, the Lo line could be used as a horizontal reference plane in CBCT generated PA cephalograms.
Subject(s)
Humans , Cone-Beam Computed Tomography , Facial AsymmetryABSTRACT
Subject(s)
Bone and Bones , Carbocyanines , Cell Culture Techniques , Coculture Techniques , Collagen , Collagenases , Drug Combinations , Durapatite , Endothelial Cells , Laminin , Lipoproteins , Magnetics , Magnets , Mandible , Mesenchymal Stem Cells , Molar, Third , Nylons , Osteoblasts , Osteogenesis , Perchlorates , Periosteum , ProteoglycansABSTRACT
INTRODUCTION: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-alpha with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1beta with different concentrations (0.01, 0.1, and 1 ng/mL) were added. RESULTS: Both TNF-alpha and IL-1beta stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-alpha and IL-1beta increased the level of ALP expression in a dose-dependent manner. Both TNF-alpha and IL-1beta also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. CONCLUSION: These results suggest that inflammatory cytokines TNF-alpha and IL-1beta can stimulate the osteoblastic activity of cultured human periosteal-derived cells.
Subject(s)
Humans , Alkaline Phosphatase , Anthraquinones , Ascorbic Acid , Bone Resorption , Cell Culture Techniques , Cytokines , Dexamethasone , Durapatite , Glycerophosphates , Homeostasis , Interleukins , Osteoblasts , Osteoclasts , Osteogenesis , Periosteum , Tumor Necrosis Factor-alphaABSTRACT
INTRODUCTION: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. MATERIALS AND METHODS: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco's modified Eagle's medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. RESULTS: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2 or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. CONCLUSION: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.
Subject(s)
Humans , Culture Media , Dental Pulp , Dental Sac , Durapatite , Flow Cytometry , Imidazoles , Immunohistochemistry , Mesenchymal Stem Cells , Molar, Third , Nitro Compounds , Polymerase Chain Reaction , Reverse Transcription , Stem Cells , Transcription FactorsABSTRACT
A melanotic neuroectodermal tumor of infancy (MNTI) is a uncommon osteolytic pigmented neoplasm that primarily affects the jaws of newborn infants. Most patients (> 90%) present with the tumor in the first year of life. Approximately 65% form in the maxilla, 11% in the mandible, 5% in the brain and elsewhere. MNTI is normally benign, but up to 15% may recur and a few have metastasized. Approximately 200 cases of MNTI have been reported but only 2 of them presented as multifocal. A case of MNTI in a 7 month old boy was encountered. The chief complaint was maxillary anterior ridge swelling. The incisional biopsy findings were MNTI. Two months after the first operation, mild swelling of another site was observed. The infant was examined periodically since undergoing two procedures with no recurrence. This case demonstrates the possibility of a multicentric MNTI. We report a multicentric MNTI with a review of the relevant literature.
Subject(s)
Humans , Infant , Infant, Newborn , Biopsy , Brain , Jaw , Mandible , Maxilla , Neuroectodermal Tumor, Melanotic , Polyenes , RecurrenceABSTRACT
Subject(s)
Female , Humans , Chin , Facial Asymmetry , Facial Bones , Head , Hypertrophy , Malocclusion , Mandibular Condyle , Open Bite , Orthognathic Surgery , Osteochondroma , Pterygoid Muscles , Skeleton , Strikes, EmployeeABSTRACT
Subject(s)
Humans , Facial Asymmetry , Incisor , Mandible , Osteotomy, Sagittal Split Ramus , Prognathism , Recurrence , Retrospective StudiesABSTRACT
Subject(s)
Humans , Alkaline Phosphatase , Ascorbic Acid , Calcium , Cell Culture Techniques , Cell Proliferation , Dexamethasone , Durapatite , Mandible , Molar, Third , Osteoblasts , Osteocalcin , Phenotype , RNA, Messenger , StrontiumABSTRACT
Subject(s)
Humans , Arteries , Cicatrix , Esthetics , Forearm , Free Tissue Flaps , Hand , Hand Strength , Head , Jugular Veins , Maxilla , Mouth , Mouth Floor , Mouth Mucosa , Neck , Palate , Parenteral Nutrition , Retrospective Studies , Sensation , Skin , Surgery, Oral , Survival Rate , Thigh , Thyroid Gland , Tissue Donors , Tongue , Transplants , WristABSTRACT
Subject(s)
Adult , Humans , Adipose Tissue , Ascorbic Acid , Bone Development , Bone Marrow , Cell Culture Techniques , Cell Separation , Collagen , Collagenases , Drug Combinations , Durapatite , Endothelial Cells , Epidermal Growth Factor , Flow Cytometry , Heparin , Hydrocortisone , Laminin , Magnetics , Magnets , Mesenchymal Stem Cells , Microspheres , Nylons , Osteoblasts , Osteotomy, Sagittal Split Ramus , Prognathism , Proteoglycans , Skin , Stem Cells , Vascular Endothelial Growth Factor AABSTRACT
Subject(s)
Humans , Collagen , Drug Combinations , Durapatite , Endothelial Cells , Laminin , ProteoglycansABSTRACT
PURPOSE: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. RESULTS: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. CONCLUSION: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
Subject(s)
Humans , Alkaline Phosphatase , Ascorbic Acid , Calcium , Cell Culture Techniques , Dexamethasone , Durapatite , Glycosaminoglycans , Homeostasis , Osteoblasts , Periosteum , Phosphotransferases , Protein Isoforms , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factor Receptor-1 , Vascular Endothelial Growth Factor Receptor-2ABSTRACT
PURPOSE: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. RESULTS: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. CONCLUSION: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
Subject(s)
Humans , Alkaline Phosphatase , Ascorbic Acid , Calcium , Cell Culture Techniques , Dexamethasone , Durapatite , Glycosaminoglycans , Homeostasis , Osteoblasts , Periosteum , Phosphotransferases , Protein Isoforms , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factor Receptor-1 , Vascular Endothelial Growth Factor Receptor-2ABSTRACT
PURPOSE: The purpose of this study is to investigate the clinical and histological features of odontogenic keratocyst PATIENTS AND METHODS: A retrosective review of 100 patients who were diagnosed as odontogenic keratocyst by hitological findings during the period of January 2000 and December 2005 in the Dept. of Oral and Maxillofacial surgery Pusan National University was consecuted. For each patient, age, sex, location of lesion, initial diagnosis by radiographic features, treatment procedure, hitologic findings and recurrance rate were evaluated. RESULTS: In this study, OKC has male prevalance to female by 1.38:1, and most likely occurs during third decade. The most common site of lesion was mandibular ramus region(34.6%) and the most common symptom was swelling(50%). The most common initial diagnosis by radiographic findings was OKC and cyst enucleation was the most common treatment method. The recurrance rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. CONCLUSION: In this study, total recurrence rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. But, since 97% of patients were treated by enucleation and adjuntive excision, further styudy is need about concordance of recurrence rate with surgical method.
Subject(s)
Female , Humans , Male , Odontogenic Cysts , Recurrence , Surgery, OralABSTRACT
PURPOSE : The purpose of this study was to examine the expression of various angiogenic factors during osteoblastic differentiation of periostealderived cells and the effects of osteogenic inductive medium of periosteal-derived cells on the proliferation of endothelial progenitor cells. MATERIALS AND METHODS : Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were divided into two groups and cultured for 21 days. In one group, the cells were cultured in the DMEM supplemented with osteogenic inductive agent, including 50g/ml L-ascorbic acid 2-phosphate, 10 nM dexamethasone and 10 mM -glycerophosphate. In the other group, they were cultured in DMEM supplemented without osteogenic inductive agent. VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1 mRNA expression was observed. Human umbilical cord blood-derived endothelial progenitor cell proliferation was also observed. RESULTS : The expression of VEGF isoforms was higher in osteogenic inductive medium than in non-osteogenic inductive medium. The expression of VEGFR-2 was also higher in osteogenic inductive medium than in non-osteogenic inductive medium. However, the expression of VEGFR-1 and neuropilin-1 was similar in both osteogenic inductive medium and non-osteogenic inductive medium. In addition, conditioned medium from differentiated periosteal-derived cells stimulated human umbilical cord blood-derived endothelial progenitor cell numbers compared to conditioned medium from non-differentiated periosteal-derived cells. CONCLUSION : These results suggest that in vitro osteoblastic differentiation of periosteal-derived cells has angiogenic capacity to support endothelial progenitor cell numbers.