ABSTRACT
Objective To explore the diagnosis and treatment strategies of Rathke's cleft cysts (RCC) in the sellar region through analyzing their imaging characteristics.Methods Thirty-two patients with RCC in the sellar region,including two also with concomitant pituitary adenoma,admitted to and performed surgery in our hospital from January 2005 to August 2011,were chosen in our study.The clinical features,especially MRI and histological appearances,were retrospectively reviewed.The experiences on their diagnosis and treatment were summarized.Results Preoperatively,only 3 patients were diagnosed or suspected as RCC.MR images revealed 10 intracystic nodules in 9 patients; their MR signal intensity was variable; not all nodules on T2-weighted images were visualized.Two nodules had bull' s eyelike changes.The hyper-intense content on T1-weighted images was not definitely associated with serious symptoms.Postoperative MRI revealed recurrence or residual lesion in 5 of 26 patients.Conclusion Intracystic nodules with homogenous intensity and non-enhancement under MRI are unique for sellar RCC; Bull's eyelike changes in the intracystic nodule under MRI might have value for confirming the diagnosis.Pituitary inflammation should not be neglected when the clinical symptoms and MRI findings of RCC are being explained.
ABSTRACT
Objective To observe the changes of c-fos protein expression in brain and beta-endorphin (β-EP) level in blood plasma in rats with diffuse brain injury (DBI) and secondary brain insult (SBI) after intraperitoneal injection of naloxone hydrochloride, and explore the role of c-fos and β-EP in development of SBI in rats. Methods Seventy health male SD rats were enrolled in the present study and randomly divided into group A (intraperitoneally injected with 0.9% saline after DBI and SBI model was reproduced), group B (injected intraperitoneally with 1.0mg/kg naloxone hydrochloride after DBI and SBI model was reproduced), and group C (intraperitoneally injected with 1.0mg/kg naloxone hydrochloride after DBI and before SBI model was reproduced). The animals were sacrificed 3, 24 and 48 hours after injury, and the number of c-fos positive cells in brain and content of β-EP in blood plasma were determined by immunohistochemistry and radioimmunoassay respectively, the water content and number of injured neurons in brain tissue were measured by pathomorphological observation of the brain tissue. Results No significant difference was observed between group B and C for all the detection parameters. In group B and C, the water content in brain tissue at 3h and 24h was found to be decreased, while the number of injured neurons at 24h and 48h increased, number of c-fos positive cells in brain at 3h, 24h and 48h decreased, and content of β-EP in blood plasma at 3h and 24h decreased when compared with group A(P<0.05). Conclusion Naloxone hydrochloride could decrease the c-fos expression in brain and β-EP level in blood plasma, alleviate the nerve injury, and protect neural function. The therapeutic effect of naloxone administered either after DBI and SBI or after DBI and before SBI was similar.
ABSTRACT
Objective To observe the changes of c-fos protein expression in brain and beta-endorphin (β-EP) level in blood plasma in rats with diffuse brain injury (DBI) and secondary brain insult (SBI) after intraperitoneal injection of naloxone hydrochloride, and explore the role of c-fos and β-EP in development of SBI in rats. Methods Seventy health male SD rats were enrolled in the present study and randomly divided into group A (intraperitoneally injected with 0.9% saline after DBI and SBI model was reproduced), group B (injected intraperitoneally with 1.0mg/kg naloxone hydrochloride after DBI and SBI model was reproduced), and group C (intraperitoneally injected with 1.0mg/kg naloxone hydrochloride after DBI and before SBI model was reproduced). The animals were sacrificed 3, 24 and 48 hours after injury, and the number of c-fos positive cells in brain and content of β-EP in blood plasma were determined by immunohistochemistry and radioimmunoassay respectively, the water content and number of injured neurons in brain tissue were measured by pathomorphological observation of the brain tissue. Results No significant difference was observed between group B and C for all the detection parameters. In group B and C, the water content in brain tissue at 3h and 24h was found to be decreased, while the number of injured neurons at 24h and 48h increased, number of c-fos positive cells in brain at 3h, 24h and 48h decreased, and content of β-EP in blood plasma at 3h and 24h decreased when compared with group A(P<0.05). Conclusion Naloxone hydrochloride could decrease the c-fos expression in brain and β-EP level in blood plasma, alleviate the nerve injury, and protect neural function. The therapeutic effect of naloxone administered either after DBI and SBI or after DBI and before SBI was similar.