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OBJECTIVE To investigate the relationship between the distribution of eosinophils(Eos) of nasal secretion and serum specific IgE(sIgE) titer in patients with allergic rhinitis, and to evaluate the diagnostic value of Eos in nasal secretion for allergic rhinitis. METHODS A total of 60 allergic rhinitis patients from Department of Otolaryngology, Beijing Shunyi Hospital of Traditional Chinese Medicine during Jan.2016 to June 2017 were selected as treatment group, and another 60 cases of non-allergic rhinitis and 30 normal persons in the corresponding time period were chosen as control group. Eos in nasal secretions and serum sIgE were measured in all the subjects and the relationship between Eos distribution and serum sIgE level was analyzed. RESULTS The Eos distribution and serum level of sIgE was consistent(κ=0.264, P=0.000) and statistical significance was found. The area of ROC of the Eos in nasal secretion was 0.881, the standard deviation was 0.025 and 95％ confidence interval was 0.841 to 0.924. The Udden index of ROC reached the maximum when the Eos in nasal secretion smear was graded as level 3, the sensitivity was 88.5％ and specificity was 99.4％. CONCLUSION Eos cytological examination of nasal secretions has some auxiliary value in the diagnosis of allergic rhinitis. It is a cheap, simple and rapid method, which can be used as an effective reference index for diagnosing allergic rhinitis in primary hospital.
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The mechanisms of food allergy include IgE-mediated and non IgE-mediated immune responses.Food intolerance is the reaction of defected digestive function caused by many factors in which the immune system is not involved.Clinically,the cases of food intolerance are increasing and the symptoms of the disease are usually complicated.Clinicians are facing the problem of differential diagnosis of food allergy and food intolerance,this article reviews the mechanisms of these two diseases to provide the reference for differential diagnosis.
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[ABSTRACT]OBJECTIVETo investigate the relationship between food allergen specific IgE, IgG and allergic rhinitis, and to explore the significance of food allergen in the diagnosis and prevention of allergic rhinitis.METHODSTen kinds of food specific IgE were detected by Western blot and 14 kinds of food specific IgG were detected by ELISA in 2860 allergic rhinitis patients. The positive results of specific IgE and specific IgG were retrospectively analyzed.RESULTSThere was no difference in positive rate of allergen in patients with different gender. Total positive rate of food specific IgE was 68.5%. The specific IgE positive rate of crab was 31.5%, shrimp 27.6%, milk 21.3%, fish assemblages 19.2%, freshwater fish assemblages 18.3% and soybean 17.2%. With the growth of age, the specific IgE positive rate of peanut increased, but eggs decreased. The total positive rate of food allergen specific IgG was 81.5%. The specific IgG positive rate of egg was 59.4%, milk 38.2%, codfish 32.6%, soybean 22.8%, rice 19% and shrimp 12.7%. With the growth of age, the specific IgG positive rate of crab increased. Specific IgE and IgG of soybean, milk, crab, shrimp and fish were correlated well.CONCLUSIONThe detection of specific food allergen antibodies is helpful for the diagnosis of allergic rhinitis.
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OBJECTIVE@#To show that Stat3 played a key role in the G1 to S phase transition in laryngocarcinoma cells.@*METHOD@#Human laryngocarcinoma cell lines Hep-2 were transfected with Stat3 antisense oligonucleotide mediated by liposome, MTT assay was used to measure the proliferation, flow cytometry was applied to analyze the cell cycle, and the expressions of Stat3, phosphorylation specific Stat3 (tyrosine705), CyclinD1, Cyclin E, CDK2, CDK4, CDK6, p21 and p27 were detected by western blot.@*RESULT@#Hep-2 laryngocarcinoma cell lines expressed constitutively activated Stat3. Antisense oligonucleotide which directed blocked up the translation site resulted in growth inhibition, downregulation of Stat3, p-Stat3, Cyclins and CDKs, and upregulation of p21 and p27.@*CONCLUSION@#Our findings suggested that Stat3 played an important role in the G1 to S phase transition in laryngocarcinoma cells, Stat3 orchestrated cell cycle by regulating the balance between CDK/Cyclin complex and CKI.
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Humans , Cell Line, Tumor , G1 Phase , Laryngeal Neoplasms , Metabolism , Pathology , S Phase , STAT3 Transcription Factor , Genetics , Metabolism , Signal Transduction , TransfectionABSTRACT
OBJECTIVE@#To investigate the expression of STAT3 and P-STAT3 and the relationship between P-STAT3 and various clinical pathological characteristics in human laryngeal carcinoma.@*METHOD@#The expression of STAT3 and its activated form P-STAT3 in tumor tissues from 50 radically resected specimens of laryngeal carcinoma and 10 normal laryngeal tissues was detected by immunohistochemical staining.@*RESULT@#The rate of protein expression of STAT3 and P-STAT3 in laryngeal carcinoma were 36/50 (72.0%) and 29/50 (58.0%) respectively, which were significantly higher than that in normal group (P < 0.01). The positive rate of P-STAT3 was closely related to the clinical stage and lymph node metastasis (P < 0.05). The expression STAT3 in laryngeal carcinoma was located in the cytoplasm of carcinoma cells, while P-STAT3 was located in the cell nucleus.@*CONCLUSION@#STAT3 and PSTAT3 were overexpressed in human laryngeal carcinoma. Activation of STAT3 was significantly related to the clinical stage and lymph node metastasis. The expression of STAT3 can be used as a significant parameter in predicting the biological behaviour of laryngeal carcinoma.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Laryngeal Neoplasms , Metabolism , Pathology , Neoplasm Staging , STAT3 Transcription Factor , MetabolismABSTRACT
OBJECTIVE To construct Hep-2 cell line with stable transfection of siRNA-STAT3 gene, and to explore the relationship between STAT3 expressionand the pathogenesis of laryngeal cancer. METHODS Specific STAT3 oligonucleotides were designed and synthesized. These oligonucleotides were annealed form the double strands DNA fragments. Then the fragments were cloned into pGPU6/GFP/Neo vector. The recombinant PGPU6/GFP/Neo-siRNA-STAT3 plasmid was confirmed by enzyme digestion and sequence analysis at the same time. Positive clones were selected out by G418 and p-STAT3 expression was identified by Western-blot analysis. The growth curve of the Hep-2 cells was drawn based on MTT assay and the growth ability of single Hep-2 cell was measured according to the plate colony formation assay respectively. RESULTS PGPU6/GFP/NEO-siRNA-STAT3 expression vector was successfully constructed. Western-blot analysis identified that p-STAT3 expression declined obviously in siRNA- STAT3 group compared with that in negative control and blank group. The growth curve explained that the Hep-2 cells of siRNA-STAT3 group began to show obvious inhibitory effect until they were inoculated in the culture plate for 3 days(P =0.001).There were pronounced inhibitory effect after 5 days(P=0.000). The results also showed time-effect relationship of the inhibition. Furthermore, the cell colony formation rate of siRNA- STAT3 group was less than the negative control and blank group(P =0.000). CONCLUSION We successfully selected out Hep-2 cell line expressed siRNA-STAT3 gene stably and efficiently in this study. Down regulation of STAT3 correlated with the inhibition of growth of Hep-2 cells.
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OBJECTIVE This study was conducted to investigate the expression of Stat3 and its target gene products including Cyclin D1 and Bcl-xl in human laryngeal cancerous tissue, and to explore the mechanism in tumorigenesis of larymgeal carcinoma, and to explore the correlation between the expression,clinical parameters as well as the pathological parameters of laryngeal carcinoma. METHODS The expression of Stat3, p-Stat3, Cyclin D1 and Bcl- xl in 50 cases of cancerous tissues, adjacent normal tissues was measured by Western blot analysis. The expression pattern of Stat3 and its activated form p-Stat3 was determined by immunohisto- chemical staining. The correlation of the expression of Stat3, p-Stat3, Cyclin D1 and Bcl-xl in laryngeal carcinoma with various clinicopathological characteristics was analyzed statistically. RESULTS The protein expression level of stat3, p-Stat3, Cyclin D1, Bcl-xl (A value)were 86.97?1.58, 56.97?3.93, 24.43?5.62, 33.78? 3.56 in laryngeal carcinoma and 36.10?0.52, 16.52?0.52, 5.94?1.75, 14.68?2.14 in adjacent normal respectively(P