ABSTRACT
Objective: To purify the lentinan from sporophore of Lentinus edodes and to study the structure and antitumor activity in vitro of lentinan. Methods: A refined lentinan, named LNT2, was isolated from the sporophore of L. edodes by alkali extraction and alcohol precipitation, and further purified by hydrogen peroxide decoloration and ultrafiltration. The molecular weight and sugar content of LNT2 were measured by HPLC and phenol-sulfuric acid method, respectively. UV was used to detect the protein and nucleic acid in LNT2, and specific rotation was detected. The chemical structure of LNT2 was determined by acid hydrolysis, periodate oxidation, methylation analysis, Fourier infrared spectrum, and NMR experiments. The chain conformation of LNT2 was evaluated by Congo-red test. The inhibitory effect of LNT2 on H22 tumor cells growth was evaluated using MTT assay. Results: The molecular weight, sugar content, and [α]D20 value of LNT2 were estimated to be 185 200, 94.99%, and +8.03°, respectively. UV spectrum showed that there were no peaks at 280 and 260 nm. Chemical and spectroscopic analyses illustrated that LNT2 had a backbone chain of β-(1→3)-linked glucopyranosyl residues and had branches of single glucosyl stubs at C-6 of terminal glucose sugar residues. Congo-red test revealed that LNT2 exhibited a triple helix comformation in low concentration of NaOH solution. Results of the antitumor activity in vitro demonstrated that LNT2 could exhibit the strong effect against the growth of H22 tumor cells and showed a dose-dependent manner. Conclusion: LNT2 is composed of β-(1→3)-linked glucan and has a triple helix comformation. Moreover, LNT2 could present the certain antitumor activities in vitro. Our study lays a solid foundation for the development and utilization of LNT2.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effects of Angelica sinensis polysaccharide-iron complex (APIC) on rats with iron deficiency anemia (IDA).</p><p><b>METHODS</b>The IDA rat model was established by adopting low-iron forage with a small amount of regular bloodletting. The rats were randomly divided into a model group, three AIPC groups (high, middle, and low dosage), an Angelica sinensis polysaccharide (ASP) group, a mixture group (ASP+FeCl(3)) and a positive control group (Niferex). Changes in hemoglobin (Hb), red blood cell count (RBC), hematocrit (HCT) and iron content of whole blood were observed.</p><p><b>RESULTS</b>There was a significant difference before and after administration in all treated groups and all indices were restored to near-normal levels in the APIC groups and the positive control group. There was a significant difference among the changes of the indices in all the APIC groups and those of the model group but not between those of the APIC groups and the positive control group. However, the recovery of the indices in the APIC groups was superior to that in the positive control group.</p><p><b>CONCLUSION</b>APIC not only has a superior therapeutic effect on IDA, but also has the effect of the ASP on supplementing blood and activating blood circulation. Hence, it may be used as a new iron-supplementing agent with a double therapeutic efficacy on blood supplementation for the treatment of IDA.</p>
Subject(s)
Animals , Female , Male , Rats , Anemia, Iron-Deficiency , Drug Therapy , Dose-Response Relationship, Drug , Drugs, Chinese Herbal , Chemistry , Therapeutic Uses , Iron , Blood , Chemistry , Therapeutic Uses , Polysaccharides , Chemistry , Therapeutic Uses , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To study some physicochemical properties of Angelica sinensis polysaccharide-iron complex (APC).</p><p><b>METHOD</b>Based on the qualitatively identified reactions of iron (III), the qualitatively identified reactions of APC were found out by comparing hydroxide. The content of iron (III) in APC was determined with iodometry. The stability of APC under physiological pH conditions was judged by titrating APC with sodium hydroxide. The deoxidization of APC was tested with colorimetric analysis.</p><p><b>RESULT</b>APC showed the qualitatively identified reactions of iron (III). The content of iron (III) in APC ranged from 10% to 40%, and the water-solubility of APC was related to the content of iron (III). The complex was stable at physiological pH from 3 to 12, without precipitation and dissociation. At 37 degrees C, Fe (III) in the complex was completely reduced to Fe (III) by ascorbic acid in about 6 hours.</p><p><b>CONCLUSION</b>APC can be qualitatively identified by using the qualitatively identified reactions of iron (III). When its iron (III) content is within 20%-25%, APC has a better ability to dissolve in water. And the complex is stable under physiological pH conditions.</p>