ABSTRACT
Objective To evaluate the value of color Doppler flow image(CDFI)in diagnosis of acute renal allograft rejection in early stage,by comparative study on CDFI,single photon emission computer tomography(SPECT)and biopsy.Methods A total of 65 patients were included in this study,including acute rejection in 26 cases,chronic rejection in 12 cases,acute tubular necrosis in 7 cases,toxic reaction of cyclosporine a in 2 cases and normal in 18 cases.They were all subjected to CDFI and biopsy,and 44 were detected by SPECT.We reviewed two-dimension gray scale picture,spectrogram,perfusion index(PI),resistant index(RI),the ratio of blood flowing velocity of systole period and diastole period(S/D) as correlated to the findings of SPECT and biopsy.Results The RI values were 0.77?0.05 and 0.72?0.07 in acute rejection group and chronic rejection group respectively,which were obviously higher than those in normal group(0.57?0.07).Conclusion CDFI is a fast,accurate and non-invasive technique in evaluating renal transplant rejection early.
ABSTRACT
Objective To observe the general status and the immunity of DBA/2 mice injected with leukemia vaccine during the immune reconstitution, and to observe the survival rate of these injected mice that were then challenged by L1210 cells. Methods Normal mice and reconstituted lymphopenic mice (RLM; DBA/2 mice rendered lymphopenic with sublethal irradiation and reconstituted with naive splenocytes) were used in the vaccination and challenge experiments with L1210 cells. Results Following vaccination, a significant increase of the IFN-? was detected in the blood of vaccinated RLM compared with that of vaccinated normal mice, as assessed by ELISA. Significantly, greater protection was induced in vaccinated RLM, which led to a stronger protection from death. Conclusion The vaccination of reconstituted lymphopenic hosts could elicit superior anti-tumor immunity compared with normal hosts, highlighting the potential clinical benefit of performing tumor vaccination during immune reconstitution.