ABSTRACT
BACKGROUND/AIMS: Oxidative stress results in protein oxidation and is implicated in carcinogenesis. Sulfiredoxin (Srx) is responsible for the enzymatic reversal of inactivated peroxiredoxin (Prx). Nuclear factor E2-related factor 2 (Nrf2) binds to antioxidant responsive elements and upregulates the expression of Srx and Prx during oxidative stress. We aimed to elucidate the biological functions and potential roles of Srx in lung cancer. METHODS: To study the roles of Srx and Prx III in lung cancer, we compared the protein levels of Nrf2, Prxs, thioredoxin, and Srx in 40 surgically resected human lung cancer tissues using immunoblot and immunohistochemical analyses. Transforming growth factor-beta1, tumor necrosis factor-alpha, and camptothecin treatment were used to examine Prx III inactivation in Mv1Lu mink lung epithelial cells and A549 lung cancer cells. RESULTS: Prx I and Prx III proteins were markedly overexpressed in lung cancer tissues. A significant increase in the oxidized form of a cysteine sulfhydryl at the catalytic site of Prxs was found in carcinogenic lung tissue compared to normal lung tissue. Densitometric analyses of immunoblot data revealed significant Srx expression, which was higher in squamous cell carcinoma tissue (60%, 12/20) than in adenocarcinoma (20%, 4/20). Also, Nrf2 was present in the nuclear compartment of cancer cells. CONCLUSIONS: Srx and Prx III proteins were markedly overexpressed in human squamous cell carcinoma, suggesting that these proteins may play a protective role against oxidative injury and compensate for the high rate of mitochondrial metabolism in lung cancer.
Subject(s)
Animals , Humans , Adenocarcinoma/enzymology , Antineoplastic Agents, Phytogenic/pharmacology , Blotting, Western , Camptothecin/pharmacology , Carcinoma, Squamous Cell/enzymology , Cell Line, Tumor , Immunohistochemistry , Lung Neoplasms/enzymology , Mink , NF-E2-Related Factor 2/metabolism , Oxidoreductases Acting on Sulfur Group Donors/genetics , Peroxiredoxin III/metabolism , Peroxiredoxins/metabolism , Prognosis , RNA Interference , Reactive Oxygen Species/metabolism , Transfection , Transforming Growth Factor beta1/metabolism , Tumor Necrosis Factor-alpha/metabolism , Up-RegulationABSTRACT
Hormone therapy for peri- and postmenopausal women is widely used for relieving vasomotor symptoms or preventing osteoporosis. Even though exogenous hormone is the most common cause of vaginal bleeding in postmenopausal women under hormone therapy, in clinical practice, physicians must never exclude the possibility of endometrial disorders, such as endometrial hyperplasia or cancer. Taking thorough medical history and performing physical and pelvic examinations are essential for the evaluation of abnormal uterine bleeding in menopausal hormone therapy. Transvaginal ultrasonography, endometrial aspiration biopsy, dilatation and curettage, and hysteroscopy are the options which physicians may use. This case is about a woman with continuous combined hormone therapy for 5 years who had experienced rather sudden onset of vaginal spotting lasting for several months and was finally diagnosed as having malignant mixed Mullerian tumor. Our purpose is to inquire into the proper approaching steps for vaginal bleeding in menopausal hormone therapy.
Subject(s)
Female , Humans , Biopsy, Needle , Dilatation and Curettage , Endometrial Hyperplasia , Gynecological Examination , Hysteroscopy , Metrorrhagia , Osteoporosis , Uterine HemorrhageABSTRACT
OBJECTIVE: The purpose of this study was to compare the effectiveness and complications between TVT and TOT in the surgical management of female stress urinary incontinence. METHODS: From December, 2005 to July, 2006, 72 patients were performed anti-incontinence surgery at our hospital. Group of TVT surgery were 35 cases and group of TOT surgery were 37 cases. We reviewed medical records and analyzed these cases about age, parity, weight, height, severity of incontinence, operation time, operation outcome, duration of hospitalization and complications. RESULTS: There were no differences in patients' mean age, parity, weight, height, menopausal status and severity of incontinence. Mean operation time of TOT group (40.2+/-30 min) was shorter than TVT group (46.7+/-32.4 min), but there was no statistical difference. In case of excluded LAVH, mean operation time of TOT group (21.4+/-9.4 min) was significantly shorter than TVT group (27.0+/-7.7 min). There were no statistical differences on mean hemoglobin drop and mean hospital stay. The objective rates of cure (88.6% vs 86.5%), improvement (5.7% vs 8.1%), and failure (5.7% vs 5.4%) were similar for the TVT and TOT groups, respectively. The subjective rates of cure (80% vs 81.1%), improvement (14.3% vs 13.5%), and failure (5.7% vs 5.4%) were similar for the TVT and TOT groups, respectively. In case of bladder perforation, TVT group (2 cases) was higher than TOT group (0 case) but there was no statistical difference. In case of vaginal erosion, urinary tract infection, pelvic hematoma, de novo overactive bladder, there were no statistical differences. CONCLUSION: The TVT and TOT are both effective surgical treatment for stress urinary incontinence. Especially, TOT is safe and time saving procedure because it needs no cystoscopy.
Subject(s)
Female , Humans , Cystoscopy , Hematoma , Hospitalization , Length of Stay , Medical Records , Parity , Suburethral Slings , Urinary Bladder , Urinary Bladder, Overactive , Urinary Incontinence , Urinary Tract InfectionsABSTRACT
OBJECTIVE: To evaluate the result of laparoscopically assisted vaginal hysterectomy (LAVH) according to history of prior abdominal surgery. METHODS: From January, 2003 to June, 2005, a total of 504 patients were performed LAVH at our Hospital. The patients were divided into 2 groups: Group of non prior abdominal surgery (Op. Hx(-) group) included 262 cases and group of prior abdominal surgery (Op. Hx(+) group) had 242 cases. We reviewed medical records and analyzed these cases regarding age, parity, weight, height, operation indication, operation outcome, duration of hospitalization and complication. RESULTS: There were no differences in terms of patients' mean age, parity, weight and height, and indications for surgery between the two groups. Mean operation time of Op. Hx(+) group (86.9+/-28.2 min) was longer than Op. Hx(-) group (80.7+/-20.0 min). There was no statistical difference on mean postoperative hemoglobin drop and mean uterine weight between the 2 groups. Mean hospital stay of Op. Hx(+) group (4.6+/-1.7 days) was longer than Op. Hx(-) group (4.3+/-0.9 days). The incidence of major surgical complications was higher in Op. Hx(+) group (10 cases - 4.1%) than Op. Hx(-) group (3 cases - 1.2%). In case of blader injury, Op. Hx(+) group (5 cases) was higher than Op. Hx(-) group (0 case). Op. Hx(+) group had 2 ureteral injuries and 1 rectal injury but there were no statistical differences. In case of trocar site bleeding, both group had 2 cases trocar site bleeding respectively. Op. Hx(-) group had 1 inferior vena cava injury but there was no statistical difference. CONCLUSION: At the time of LAVH, the incidence of bladder injury was higher in group of patients with history of prior abdominal surgery. So special attention should be paid to prevent bladder injury.
Subject(s)
Female , Humans , Hemorrhage , Hospitalization , Hysterectomy, Vaginal , Incidence , Length of Stay , Medical Records , Parity , Surgical Instruments , Ureter , Urinary Bladder , Vena Cava, InferiorABSTRACT
OBJECTIVE: To evaluate the result of laparoscopically assisted vaginal hysterectomy (LAVH) according to history of prior abdominal surgery. METHODS: From January, 2003 to June, 2005, a total of 504 patients were performed LAVH at our Hospital. The patients were divided into 2 groups: Group of non prior abdominal surgery (Op. Hx(-) group) included 262 cases and group of prior abdominal surgery (Op. Hx(+) group) had 242 cases. We reviewed medical records and analyzed these cases regarding age, parity, weight, height, operation indication, operation outcome, duration of hospitalization and complication. RESULTS: There were no differences in terms of patients' mean age, parity, weight and height, and indications for surgery between the two groups. Mean operation time of Op. Hx(+) group (86.9+/-28.2 min) was longer than Op. Hx(-) group (80.7+/-20.0 min). There was no statistical difference on mean postoperative hemoglobin drop and mean uterine weight between the 2 groups. Mean hospital stay of Op. Hx(+) group (4.6+/-1.7 days) was longer than Op. Hx(-) group (4.3+/-0.9 days). The incidence of major surgical complications was higher in Op. Hx(+) group (10 cases - 4.1%) than Op. Hx(-) group (3 cases - 1.2%). In case of blader injury, Op. Hx(+) group (5 cases) was higher than Op. Hx(-) group (0 case). Op. Hx(+) group had 2 ureteral injuries and 1 rectal injury but there were no statistical differences. In case of trocar site bleeding, both group had 2 cases trocar site bleeding respectively. Op. Hx(-) group had 1 inferior vena cava injury but there was no statistical difference. CONCLUSION: At the time of LAVH, the incidence of bladder injury was higher in group of patients with history of prior abdominal surgery. So special attention should be paid to prevent bladder injury.
Subject(s)
Female , Humans , Hemorrhage , Hospitalization , Hysterectomy, Vaginal , Incidence , Length of Stay , Medical Records , Parity , Surgical Instruments , Ureter , Urinary Bladder , Vena Cava, InferiorABSTRACT
Idiopathic tracheal stenosis is a type of benign stenosis that possesses specific characteristics but is of unknown origin. It is a rare disease characterized by extensive fibrosis of a portion of trachea, and predominantly found in women. The lesion presents as circumferential fibrotic stenosis that usually occurs at upper trachea and the subglottic larynx, but lower trachea may also be involved. Diagnosis is made from the clinical characteristics accompanied by compatible pathologic features and by exclusion of other etiologies. Conservative management such as laser resection, dilatation and stent insertion can be tried initially, but surgical resection is recommended for definitive treatment due to frequent restenosis and maintenance problems of conservative approach. We report a case of idiopathic tracheal stenosis treated with tracheal resection and anastomosis followed by insertion of a retrievable stent for immediate relief of airway obstruction.
Subject(s)
Female , Humans , Airway Obstruction , Constriction, Pathologic , Diagnosis , Dilatation , Fibrosis , Larynx , Rare Diseases , Stents , Trachea , Tracheal StenosisABSTRACT
Broncho-esophageal fistula(BEF) is an uncommon clinical entity which can cause severe suppurative lung disease. Acquired fistulas between the esophagus and tracheobronchial tree are relatively uncommon. They are caused by many diseases including malignancy and chronic inflammation such as tuberculosis and have favorable outcome with proper treatment. To our knowledge, there has been no description of patients with BEF due to the bronchiectasis. We report a case of broncho-esophageal fistula in association with bronchiectasis in a 35-year-old male patient with hemoptysis. Bronchoscopy revealed mild bleeding from the superior segment of the right lower lobe without specific endobronchial lesion. Barium esophagogram could not confirm the fistula. The diagnosis of a broncho-esophageal fistula was established by an esophagogastroscopy using fistulogram and subsequent bronchoscopy, in which the communication between the bronchial tree and the esophagus was demonstrated by instilling dye selectively through the fistulous opening using esophagogastroscopy and visualizing the fistula and the bronchial tree. The patient was treated with resection of the right lower lobe, extirpation of the diverticulum and surgical closure of the bronchial defect and fistula, but he suffered from pneumonia thereafter and eventually expired due to sepsis and multiple organ failure.
Subject(s)
Adult , Humans , Male , Barium , Bronchiectasis , Bronchoscopy , Diagnosis , Diverticulum , Esophagus , Fistula , Hemoptysis , Hemorrhage , Inflammation , Lung Diseases , Multiple Organ Failure , Pneumonia , Sepsis , TuberculosisABSTRACT
Tyrosinemia type 1 is an autosomal recessive disorder caused by deficiency of the enzyme fumarylacetoacetate hydrolase(FAH). The disease is characterized by hepatic dysfuntion, hepatocellular carcinomas, renal tubular dysfunction, rickets, and neurologic crises. Two forms of the disease, acute and chronic, are thought to be from the residual enzyme activity in the liver. The diagnosis of the tyrosinemia type 1 is suggested by elevated plasma tyrosine, supported by increased urinary succinylacetone, and confirmed by reduced FAH activity in cultured fibroblasts. We had a 5 month old Korean boy with acute tyrosinemia type 1 who presented with recurrent sepsis-like episodes since 2 months of age, progressive liver dysfunction, and rickets. Plasma amino acid analysis showed markedly elevated tyrosine, methionine and urine amino acid analysis was suggestive of Fanconi syndrome showing generalized aminoaciduria. Organic acid analysis by Gas Chromatography/Mass Spectrometry detected large amount of succinylacetone excreted in the urine. Delta-aminolevulinic acid was elevated as well. X-ray findings were characteristics of rickets and abdominal sonogram, CT and MRI revealed cirrhotic liver with varying size of multiple nodules. Liver transplantation was strongly recommended throughout his clinical course but refused by parents, and he died of hepatic failure at the age of 8 months. Autospy was perfomed showing macro and micronodular liver cirrhosis. Kidney was markedly enlarged, however, glomeruli and tubules were relatively unaltered. Mutation analysis is under the study.
Subject(s)
Humans , Infant , Male , Acute Disease , Aminolevulinic Acid , Carcinoma, Hepatocellular , Diagnosis , Fanconi Syndrome , Fibroblasts , Kidney , Liver , Liver Cirrhosis , Liver Diseases , Liver Failure , Liver Transplantation , Magnetic Resonance Imaging , Methionine , Parents , Plasma , Rickets , Spectrum Analysis , Tyrosine , TyrosinemiasABSTRACT
Lymphangioma of the stomach is known to be extremely rare, benign tumor, reported only 13 cases now, worldwidely, The lesions are soft, sponge like, and pinkish colored and filled with watery fluid exudates. The histologic examination reveals that lymphangiomas are composed of endothelium-lined spaces that contain a eosinophilic protein-rich fluid. They usually present as polypoid lesions because they are originated from submucosal layer. By endoscopy, they appear as smooth, soft, polypoid submucosal mass. The endoscopic ultrasonographic findings of gastric lyrnphangioma were cystic mass with multi-septation originated from submucosal layer of the stomach. Recently, We experienced a case of lymphangioma associated with early gastric cancer of the stomach. So we report this case with brief review of world literature.
Subject(s)
Endoscopy , Eosinophils , Exudates and Transudates , Lymphangioma , Porifera , Stomach , Stomach NeoplasmsABSTRACT
Implantation of malignant cells along the needle aspiration tract in patients with lung cancer is a rare but potential complication following percutaneous fine needle aspiration biopsy. Dissemination of cancer cells by aspiration biopsy can change resectable, potentially curable lung cancer to unresectable cancer. We report a 55 year male patient who underwent completion pneumonectomy due to squamous cell carcinoma and one cycle of chemotherapy. He developed outgrowing chest wall tumor at the site of needle aspiration biopsy performed prior to completion pneumonectomy and was pathologically diagnosed as metastatic squamous cell carcinoma. The lesion was successfully treated by radical full-thickness resection of the chest wall and reconstruction with latissimus dorsi musculocutaneous island flap.
Subject(s)
Humans , Male , Biopsy, Needle/adverse effects , Carcinoma, Squamous Cell/secondary , Lung Neoplasms/pathology , Middle Aged , Neoplasm Seeding , Thoracic Neoplasms/secondaryABSTRACT
No abstract available.