Inhibitory effect of Biejiajian pills on HepG2 cell xenograft growth and expression of β-catenin and Tbx3 in nude mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the molecular mechanism by which Biejiajian pills inhibit hepatocellular carcinoma in a nude mouse model bearing HepG2 cell xenograft.</p><p><b>METHODS</b>The inhibitory effect of Biejiajian pills on the growth of HepG2 cell xenograft in nude mice was observed. Immunohistochemical method was used to examine proliferating cell nuclear antigen (PCNA) expression in HepG2 cell xenograft, and TUNEL method was employed to detect the cell apoptosis; the expression levels of β-catenin and Tbx3 were measured by Western blotting.</p><p><b>RESULTS</b>Biejiajian pills significantly suppressed the growth of HepG2 cell xenograft in nude mice. The tumor-bearing mice treated with a high and a moderate dose of Biejiajian pills showed significantly increased apoptosis rate of the tumor cells [(22.9±1.220)% and (14.7±0.50)%, respectively] compared with the control group [(5.5±0.90)%, P<0.05]. Treatment with Biejiajian pills significantly decreased the expressions of PNCA, β-catenin, and Tbx3 in the cell xenograft (P<0.05).</p><p><b>CONCLUSIONS</b>Biejiajian pills can inhibit the growth of HepG2 cell xenograft in nude mice and promote tumor cell apoptosis possibly by inhibiting PNCA expression and the Wnt/β-catenin signaling pathway.</p>

[Microscopic examination in quantifying of Malassezia yeast in scalp and rapid diagnosis of fungi invasive condition: a brief report]

Malassezia Species are often commensal of the human skin and scalp that opportunistically in exist of particular predisposing factors, their proliferation increases; as, in dandruff and seborrheic dermatitis which both togather affect more than 50% of humans, the excess proliferation of yeast in scalp, leads to scalp-flaking and causes physical and mental disorder in peaple, spacially in youth that their health and hiar hygiene and beauty is more important for them. Thus, this survey has been done for rapid, easy and inexpensive method to diagnosis of abnormal proliferation and invasive condition of Malassezia yeast and can be more benefical for proper treatment. Sampling with scalpel scraping from scalp of volunteer persons that had not bathed at least two day ago were done and preparation of direct microscopic slides and staining with methylene blue were accomplished. Then, survey of morpholgic characteristics, yeast quantification and mycelium detection were done by direct microscopic examination. From 140 scalp samples of adult persons of both gender [male and female] with different age groups, observation of malassezia yeast in 93.5% [131] were positive and 6.5% [9] were negative in direct microscopic examination. Results of yeast quantification in positive cases were: mild or normal flora 25.2%, intermediate 24.5%, severe 50.3%. Detection of mycelium in positive cases were 22.9% [30] [P=0.007 df=2]. Application of an accessible, easy and inexpensive method and a determinated pattern [yeast quantification with direct microscopic examination] to distinguish normal flora from abnormal condition [excess proliferation and mycelium production] in cases of Malassezia yeasts can be more useful to rapid diagnosis of abnormal proliferation and invasive condition in order to initiate a proper antifungal treatment.

Hydrocamptothecin promotes the mRNA expression of Wnt signaling inhibitor DKK-1 / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effects of hydrocamptothecin on the expression of Wnt signaling pathway inhibitor DKK-1 in tumor cells.</p><p><b>METHODS</b>Human HepG2, Hep3B, LoVo and U251 cells were treated with the antitumor drug Hydrocamptothecin. DKK-1 mRNA expression in the cells was detected with RT-PCR, and beta-catenin expression was measured by fluorescence-activated cell sorting (FACS).</p><p><b>RESULTS</b>DKK mRNA in Hep3B, HepG2, LoVo and U251 cells was significantly increased after hydrocamptothecin treatment for 24 h, and the percentage of beta-catenin-positive cells and fluorescence intensity for beta-catenin expression was lowered in the cells after the treatment.</p><p><b>CONCLUSION</b>Hydrocamptothecin promotes mRNA expression of Wnt signaling pathway inhibitor DKK-1 in Hep3B, HepG2, LoVo and U251 cells.</p>

Study on the Preparation of Sulfadiazine Silver Collagen Sheeting for Burn and the Release Rate of Sulfadiazine-silver / 中国药房

AIM:To prepare the sulfadiazine silver collagen sheeting for burn(SD-Ag sheeting) and determine the release rate of sulfadiazine silver METHODS:To prepare the SD-Ag sheeting by cross-linking method and to determine the release rate by uniform design RESULTS:The release rate of SD-Ag from SD-Ag sheeting was (22 38?0 036)% CONCLUSION:The preparation of SD-Ag sheeting by cross-linking method was convenient The determining method was accurate,rapid and simple for the quality control of the SD-Ag sheeting