Association between physical activity and exercise self-efficacy for adolescents with visual impairment / 中国康复理论与实践

ObjectiveTo investigate the association between physical activity (PA), sedentary level and exercise self-efficacy for adolescents with visual impairment, and impacts of gender, age, body mass index (BMI), degree of visual impairment and exercise self-efficacy on PA. MethodsFrom October to December, 2022, 79 students aged 13 to 18 years with visual impairment from Nanjing School for the Blind were measured PA and sedentary level with accelerometer. The average percentage of daily sedentary time (ST), light physical activity (LPA) and moderate-to-vigorous physical activity (MVPA) were recorded. The students fininshed the Exercise Self-Efficacy Questionnaire with the help of teachers, and their height and body mass was measured. ResultsThe average percentage of daily LPA, MVPA and ST was (15.45±5.99)%, (4.55±2.15)% and (80.00±6.99)%, respectively. Compared with the girls, the boys spent more time in LPA (F = 5.330, P < 0.05) and less in ST (F = 5.760, P < 0.05). Compared with students aged 16 to 18 years, those aged 13 to 15 years spent more time in MVPA (F = 11.292, P < 0.001) and less in ST (F = 8.279, P < 0.01). The score of exercise self-efficacy correlated with ST, LPA, MVPA and age (|r| > 0.254, P < 0.05). The result of multiple linear regression showed that the exercise self-efficacy (β = 0.224) and age (β = -0.495) were the independently factors related with MVPA, which explained 34.7% of MVPA variation. ConclusionAdolescents with visual impairment aged 13 to 18 years spend more time in sedentary, and their PA level is very low. Improvement of exercise self-efficacy may help to improve PA level of them.

To explore the effects of blood-letting at jing points and auricular tips on body temperature and inflammatory factors in emergency patients with coronavirus disease 2019 based on real world / 中国中西医结合急救杂志

Objective To observe the effect of exsanguination at jing points and auricular tips on antipyretic effect and inflammation factors level in patients with fever caused by coronavirus disease 2019(COVID-19).Methods A retrospective study was conducted.A total of 521 febrile patients with COVID-19 admitted to the Affiliated Hospital of Nanjing University of Chinese Medicine from January 1 to 10,2023 were selected as the study subjects.The patients were divided into the control group(treated with conventional treatment)and the experimental group(treated with exsanguination of jing points and auricular tips based on the control group)according to whether they received traditional Chinese medicine(TCM)exsanguination treatment.The body temperature before treatment and 30 minutes and 4 hours,24 hours after treatment,the immediate(within 30 minutes)antipyrexia efficiency,the time of body temperature returning to normal,and the levels of inflammatory factors before treatment and 48 hours after treatment were collected,and the differences between the two groups were compared.According to the standard of fever relief≥1℃within 4 hours,the differences of the average onset time of fever and the rate of fever relief at 2.5,3 and 4 hours after treatment were compared between the two groups.Kaplan-Meier curve was drawn to analyze the change of 4-hour antipyrexia rate in the two groups before and after matching.Results A total of 169 cases in the experimental group and 352 cases in the control group were finally included.Because this study was retrospective,there were significant differences in the course of disease and underlying diseases(chronic bronchitis,diabetes,renal insufficiency,hepatobiliary diseases)between the two groups at baseline(all P＜0.05).In order to reduce the differences,the above 4 factors were included in the predictor variables,and a 1∶1 propensity matching was performed to match 166 pairs of case samples,which were comparable.The result showed body temperature of the two groups gradually decreased with the extension of time,and the body temperature of the experimental group was significantly lower than that of the control group at each time point(℃:30 minutes was 38.32±0.22 vs.38.43±0.24,4 hours was 37.67±0.32 vs.38.13±0.51,24 hours was 37.03±0.38 vs.37.14±0.51,all P＜0.05),the immediate antipyrexia efficiency was significantly higher than that of the control time group[63.86%(106/166)vs.44.58%(74/166),P＜0.05],and the time of body temperature recovery to normal was significantly shorter than that of the control group(hours:19.25±7.79 vs.38.90±17.17,P＜0.05).In terms of the 4 hours antipyretic onset dynamic time,the average antipyretic onset time of the experimental group was significantly shorter than that of the control group[hours:4.39(4.11,4.68)vs.7.16(6.71,7.61),P＜0.05],the fever relief rates at 2.5,3 and 4 hours were significantly higher than those in the control group[6.63%(11/166)vs.0.60%(1/166),24.10%(40/166)vs.4.82%(8/166),74.10%(123/166)vs.38.55%(64/166),respectively,all P＜0.05].Kaplan-Meier curve analysis showed that there were statistically significant differences in 4 hours fever reduction rates between the two groups before and after matching(all P＜0.05).In terms of inflammatory factors,high-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the two groups after treatment were significantly lower than those before treatment[hs-CRP(mg/L):control group was 32.03±15.38 vs.79.78±13.34,experimental group was 33.21±16.07 vs.80.03±14.66;TNF-α(μg/L):control group 2.91±0.88 vs.5.09±0.95,experimental group 2.83±0.98 vs.5.10±0.87;IL-6(ng/L):control group was 96.30±48.12 vs.206.48±56.70,experimental group was 94.01±45.13 vs.202.38±55.38,all P＜0.05],but there was no significant difference in the levels of the above indicators between the two groups after treatment.Conclusion Exsanguination at the jing points and auricular tips has a good antipyretic effect in the treatment of fever caused by COVID-19,which can accelerate the antipyretic time,improve the antipyretic efficiency,and reduce the level of inflammatory factors.

Heat stroke induces oncosis rather than apoptosis in skeletal muscle cells / 解放军医学杂志

Objective To investigate the oncosis of skeletal muscle cells during heatstroke, and the mechanism severe heatstroke associated with rhabdomyolysis. Methods The human skeletal muscle cells (HSKMC) were divided into control group and heat shock group. The control group was incubated at 37 ℃, 5% CO2 cell incubator, while the heat shock group was exposed at 43 ℃, 5% CO2 cell incubator for 2 hours, followed by incubating at 37 ℃, 5% CO2 cell incubator. At incubation point of 0, 6, 12, and 24 hours, the CCK-8 method was used to detect cell viability; the LDH method was used to detect cytotoxicity; the transmission electron microscope was used to detect cell ultrastructure changes; Annexin -FITC/PI double staining method was used to detect double-positive cell rate; Western blotting method was used to detect porimin and caspase-3 protein expression. Results Compared with the control group, HSKMC cell viability decreased with cytotoxicity increased at 0 h after rewarming in a time-dependent manner (P0.05). Conclusion Heat stroke-induced oncosis rather than apoptosis of skeletal muscle cells.

Twoyear outcomes and the influence factors of indirect pulp treatment in primary teeth: a retrospective study / 北京大学学报(医学版)

OBJECTIVE@#To evaluate the outcomes and to find out the influence factors of indirect pulp treatment in primary teeth.@*METHODS@#Children who received indirect pulp treatment in primary teeth in the Department of Pediatric Dentistry in Peking University School and Hospital of Stomatology from August, 2014 to September, 2016 were collected in the Electronic Medical Record Database of Peking University School and Hospital of Stomatology, via the database for scientific research provided by the corporation of Kaientai. The children selected as the subjects of this study were followed up over 1.5 years, and they were under 9 years old if in the group of primary molars while the children in the group of primary anterior teeth were under 4 and a half years old. Those children who were not reviewed regularly or didn't have complete medical records were removed. Basic information, the relevant medical records and radiographic records of those children were collected. All teeth were examined clinically and classified into 2 outcomes, teeth in group H were regarded as succeeded, and teeth in group P were regarded as failed. Survival analysis was applied. The survival rate and survival time of the deciduous teeth calculated. Multivariate analysis was performed by using Cox proportional hazard model.@*RESULTS@#One hundred and six children were finally included, aged from 1.6 to 8.8 years, with the mean age of (5.0±1.7) years. 168 primary teeth (122 primary molars, 46 primary anterior teeth) were included, and the average follow up time was (729±244) days. Thirty-five primary teeth (23 primary molars, 12 primary anterior teeth) failed upon clinical or radiographic examinations by September, 2018. The cumulative survival probability of half a year, one year, one year and a half, two years, two and a half years for the indirect pulp treatment was 93.5%, 92.9%, 87.5%, 82.7%, and 75.5% through the KaplanMeier method, respectively. Through the analysis of Cox proportional hazard model, in primary molars, the survival probability tended to be lower when the number of tooth surface affected by caries was greater (OR=1.709, P<0.05). Compared with primary molars, the survival probability of primary anterior teeth was lower, but the difference was not significant.@*CONCLUSION@#Complying the current instructions in our department, the cumulative survival probability of two and a half years after the indirect pulp treatment in primary teeth was 75.5%. In primary molars, the survival probability tended to be lower when the number of tooth surfaces affected by caries increased.

Two-year outcomes of pulpotomy in primary molars using mineral trioxide aggregate: a retrospective study / 北京大学学报(医学版)

OBJECTIVE@#To evaluate the two-year outcomes of primary molars pulpotomy using mineral trioxided aggregate (MTA) and to find out the potential influence factor, with the help of electronic medical record database.@*METHODS@#Children who received primary molars pulpotomy in the Department of Pediatric Dentistry in Peking University School and Hospital of Stomatology from May, 2014 to November, 2015 were searched in the Electronic Medical Record Database of Peking University School and Hospital of Stomatology, via the database for scientific research provided by the corporation of Kaientai. The children who were healthy, no more than 8 years old and followed up over 1.5 years were selected as the subjects of this study. At the same time, those children who didn't have complete medical records and X-rays before and after treatment were removed. Basic information, the relevant medical records and radiographic records of those children were collected. All molars were examined clinically and radiographically, and classified into 1 of 5 outcomes: N, H, P0, PX, PY. Molars classified into P0, PX and PY were regarded as failed. Survival analysis was applied. The survival rate and survival time of the deciduous teeth were calculated.Multivariate analysis was performed by using Cox proportional hazard model.@*RESULTS@#One hundred and fifteen children were finally included,aged from 2.6 to 8.2 years, with the mean age of (4.5±1.1) years. 211 primary molars were included, and the average follow up time was (880±154) days. A good level of agreement between the raters was found for molars with five outcomes(κ=0.913). Intrarater reliability was good for molars with five outcomes (κ=0.916). Forty-nine molars failed by September, 2017. Forty-three molars had abnormal radiographic manifestation. Six molars suffered premature loss. Only fourteen molars had an associated gingival swelling or parulis, or pathologic mobility upon clinical examination. The cumulative survival probability of half a year, one year, one year and a half, two years, two year and a half for the pulpotomy was 100%, 98.5%, 92.9%, 90.5%,73.8% through the Kaplan-Meier method, respectively. Through the analysis of Cox proportional hazard model, the survival probability was significantly higher when the age was younger. When sodium hypochlorite and the saline were used as the irrigation at the same time, the survival probability was not improved for those teeth with more bleeding in the operation compared with that used saline only. The teeth with preformed metal crown (PMC) gained longer median survival time than those restored with resin composite and others, but the difference was not significant, either. What's more, gender, the restoration time of PMC, the restoration of the opposite teeth were not found to be associated with the success of the treatment.@*CONCLUSION@#The cumulative survival probability of two years after the primary pulpotomy was 90.5%, complying the current instructions in our department. The survival probability of the molars after the treatment was significantly affected by the age.

Effect of APP based continuous nursing on the quality of life of children with cerebral palsy / 中国实用护理杂志

Objective To discuss the use of the APP to continue nursing effect on the quality of life of children with cerebral palsy. Methods A total of 100 cases of cerebral palsy hospitalized in July to December of 2016 were selected as the observation group, and the continuation nursing based on"Xinjiang cerebral palsy mobile APP"was carried out. A total of 102 cases of cerebral palsy were used as the control group in the hospital in January to June of 2016, and the traditional continuation nursing was carried out, and the self-care ability and quality of life of the children were evaluated by Activity of Daily Living Scale (ADL), Pediatric Quality of Life Inventory TM3.0-Rheumatology (PedsQLTM3.0) on admission hospital, discharge and 6 months after discharge. Results The time, time and group interaction of 2 groups of children with cerebral palsy in ADL score were statistically significant (F=231.819, 33.382, P<0.01). The ADL score of 6 months after discharge in the observation group was (66.100 ± 7.368) points, the control group was (59.230 ± 10.534) points, and there was statistically significant (t=3.802, P<0.01). The time, group and time group interaction of 2 groups of children with cerebral palsy in PedsQLTM3.0 score were statistically significant (F=23.398, 234.374, 149.209, P<0.01). There was a statistically significant difference in the observation group between admission hospital and discharge, 6 months after discharge and discharge (t=-3.384,-4.198, P<0.05 or 0.01). The difference was statistically significant in control group between discharge and admission hospital(t =-17.809, P <0.01), but there was no significant difference between 6 months after discharge and discharge (P> 0.05). Conclusions Based on the continuation of"Xinjiang cerebral palsy mobile APP", nursing can improve the ability to care for children with cerebral palsy life, help children improve quality of life. Worthy of further promotion.

Biomineralization of a calcifying ureolytic bacterium Microbacterium sp. GM-1

Background: Biomineralization is a significant process performed by living organisms in which minerals are produced through the hardening of biological tissues. Herein, we focus on calcium carbonate precipitation, as part of biomineralization, to be used in applications for environmental protection, material technology, and other fields. A strain GM-1, Microbacterium sp. GM-1, isolated from active sludge, was investigated for its ability to produce urease and induce calcium carbonate precipitation in a metabolic process. Results: It was discovered that Microbacterium sp. GM-1 resisted high concentrations of urea up to 60 g/L. In order to optimize the calcification process of Microbacterium sp. GM-1, the concentrations of Ni2+ and urea, pH value, and culture time were analyzed through orthogonal tests. The favored calcite precipitation culture conditions were as follows: the concentration of Ni2+ and urea were 50 µM and 60 g/L, respectively, pH of 10, and culture time of 96 h. Using X-ray diffraction analysis, the calcium carbonate polymorphs produced by Microbacterium sp. GM-1 were proven to be mainly calcite. Conclusions: The results of this research provide evidence that Microbacterium sp. GM-1 can biologically induce calcification and suggest that strain GM-1 may play a potential role in the synthesis of new biominerals and in bioremediation or biorecovery.

An UHPLC-MS/MS method for simultaneous determination of quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, bilobalide and ligustrazine in rat plasma, and its application to pharmacokinetic study of Xingxiong injection / 中国天然药物

The present study was designed to develop and validate a rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of five major active constituents in the traditional Chinese medicinal preparation Xingxiong injection (XXI) in rat plasma, including quercetin 3-O-rutinoside (QCR), kaempferol 3-O-rutinoside (KFR), isorhamnetin 3-O-rutinoside (ISR), bilobalide (BB), and ligustrazine (LGT). The plasma samples were pretreated by protein precipitation with acetonitrile. The chromatographic separation was achieved on a Waters Symmetry C analytical column (2.1 mm × 100 mm, 3.5 μm) with a mobile phase of 0.1% aqueous formic acid (A)-acetonitrile (B). Quantitation of the five bioactive constituents was achieved. Naringin was used as the internal standard (IS). All the calibration curves showed good linearity (r > 0.996) over the concentration range, with the lowest limit of quantification (LLOQ) between 2-18 ng·mL. The intra- and inter-day accuracy and precision of the analytes were both within acceptable limits. Moreover, satisfactory extraction recoveries (90.92%-104.03%) were obtained by protein precipitation. The validated method was successfully applied to a pharmacokinetic study of XXI in rats after intravenous administration at three doses. The pharmacokinetic parameters of the five compounds varied in a dose-dependent manner within the tested dosage range. The present study was the first report of pharmacokinetic study for XXI.

Analysis on the situation of human resource flow for centers of disease control and prevention in China / 上海预防医学

Objective To propose the policy recommendations to optimize the construction of human resources in centers for disease control and prevention , thus promoting the healthy development of China’s disease control and prevention undertaking . Methods The data between 2011 and 2015 from China Health Yearbook and China Yearbook of Health and Family Planning Statistics together with the monitoring, evaluation and investigation data from the long-term plan for health talents were utilized for making quantitative analyses;3 representative provinces were selected for making qualitative interviews . Results Between 2010 and 2014 , the average annual growth rate for the number of personnel in disease control and prevention centers was -0.40%.The average annual growth rate for the number of health technical personnel was -0 .87%, and that for the number of practicing/assistant physicians was-2.36%. Conclusion The loss of professional cores does occur in the human resources for disease prevention and control in China .The construction of the human resources for disease prevention and control shall be effectively strengthened and relevant measures shall be taken to attract and retain related technical specialists and talents .

Comprehensive analysis of chemical constituents in Xingxiong injection by high performance liquid chromatography coupled with mass spectrometry / 中国天然药物

Xingxiong injection (XXI) is a widely used Chinese herbal formula prepared by the folium ginkgo extract and ligustrazine for the treatment of cardiovascular and cerebrovascular diseases. Compared with the pharmacological studies, chemical analysis and quality control studies on this formula are relatively limited. In the present study, a high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) method was applied to comprehensive analysis of constituents in XXI. According to the fragmentation rules and previous reports, thirty ginkgo flavonoids, four ginkgo terpene lactones, and one alkaloid were identified. A high performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QQQ MS) method was then applied to quantify ten major constituents in XXI. The method validation results indicated that the developed method had desirable specificity, linearity, precision and accuracy. The total contents of ginkgo flavonoids were about 22.05-25.51 μg·mL(-1) and the ginkgo terpene lactones amounts were about 4.41-8.70 μg·mL(-1) in six batches of XXI samples, respectively. Furthermore, cosine ratio algorithm and distance measurements were employed to evaluate the similarity of XXI samples, and the results demonstrated a high-quality consistency. This work could provide comprehensive information on the quality control of Xingxiong injection, which be helpful in the establishment of a rational quality control standard.

Changes of p38 MAPK and nuclear factor-kappa B in lung tissue of acute paraquat poisoned rats / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate NF-kappaB activity and the expression of phosphorylated p38 MAPK protein in lung tissue of acute paraquat poisoned rats and the effect of MT.</p><p><b>METHODS</b>One hundred and twenty-eight Sprague-Dawley (SD) rats were randomly divided into three experimental groups: poisoned group, MT group and control group. On the 1st, the 3rd, the 7th and the 14th day after exposure, levels of malondialdehyde (MDA) in serum were detected, NF-kappaB activity in the lung tissues was assessed by electrophoresis mobility shift assay (EMSA), the expression of the phosphorylated p38 MAPK was evaluated by Western blot method, the lung pathological changes of rats were observed.</p><p><b>RESULTS</b>The level of malondialdehyde (MDA) in serum increased significantly in poisoned group on the 1st day (4.45 +/- 1.23), the 3rd day (3.77 +/- 1.12) and the 7th day (2.84 +/- 0.96) nmol/ml compared with that in control group (1.36 +/- 0.52) nmol/ml (P < 0.01). There was a significant decrease in MT group on the 1st day (2.68 +/- 0.85), the 3rd day (1.97 +/- 0.74) and the 7th day (1.53 +/- 0.62) nmol/ml compared with poisoned group (P < 0.05). The expression of the phosphorylated p38 MAPK and NF-kappaB activity in lung tissue of poisoned group significantly increased compared with control group (P < 0.01). There was a significant decrease in NF-kappaB activity and expression of the phosphorylated p38 MAPK in the lung tissues in MT group compared with poisoned group (P < 0.05).</p><p><b>CONCLUSION</b>NF-kappaB and p38 MAPK could play an important role in lung injury of poisoned rats. MT may inhibit the expression of NF-kappaB and phosphorylated p38 MAPK, and therefore might have the therapeutical effect on acute paraquat poisoning.</p>

Comparison of fracture resistance of pulpless teeth restored with fiber reinforced composite posts and three kinds of resin core material / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the fracture resistances of pulpless teeth restored with FRC (Fiber Reinforced Composite) posts and three kinds of resin core material.</p><p><b>METHODS</b>A total of 42 recently extracted upper incisors were randomly divided into 3 groups. Group A was restored with prefabricated glass-fiber posts and Artglass polymer core; group B with prefabricated glass-fiber posts and Charisma composite resin core; and group C with prefabricated glass-fiber posts and AB composite resin core. In every group, the core material was processed by hot-press and non hot-press respectively. The posts size and shape were identical in the 3 groups. All teeth were fully covered with polycarbonate resin crowns. Fracture resistance was measured by applying point force at 130 degrees to the long axis of the teeth on an universal testing machine.</p><p><b>RESULTS</b>Mean fracture threshold was 505.4 N +/- 42.0 N and 564.1 N +/- 41.7 N in group A, 411.3 N +/- 23.3 N and 315.3 N +/- 19.1 N in group B and 358.4 N +/- 36.1 N and 423.4 N +/- 47.5 N in group C. In all groups, there was no posts fracture and polycarbonate resin crowns fragmentation.</p><p><b>CONCLUSION</b>The composite restoration of FRC posts combined with resin core and resin crown can improve the fracture resistance of the pulpless roots. The strength of resin core material can be increased by hot-press methods.</p>