ABSTRACT
Objective:To observe the effects of recipes for tonifying kidney and replenishing Qi, Zuoguiwan (ZG) and Yiqi Congming Tang(YQ) on memory capacity, expressions of learning and memory-related genes expression, and explore the changes in relevant epigenetic modification enzymes. Method:SD male rats with natural aging (24 months old) were used as animal models and randomly divided into aged control group, aged ZG group(12.12 g·kg-1), aged YQ group(10.18 g·kg-1), aged compound group(11.15 g·kg-1) and aged antagonist RU38486 group(5×10-3g·kg-1). Another 5 months old male SD rats were included as the young control group. Morris water maze method was used to observe the spatial learning and memory ability of the rats. The co-localizations of histone deacetylase 2 (HDAC2) and methyl CpG binding protein 2 (MeCP2) in hippocampus of rats in each group were observed by laser confocal microscope. The changes in expressions of glucocorticoid receptor (GR), synapsin1(Syn-1), HDAC2, and histone acetyltransferase 1(HAT1) proteins in hippocampus of each group were detected by Western blot, and mRNA expression of HDAC2 was detected by Real-time fluorescence quantitative polymerase Chain reaction (Real-time PCR). At the same time, the effects of ZG, YQ and compound decoction in alleviating the above-mentioned abnormal changes were observed. Result:Compared with the young control group(control group), the latency of the aged control group was significantly prolonged (PPPPPPPConclusion:ZG group, YQ group, and compound group can improve the spatial learning and memory abilities of aged rats by increasing the expression of learning-memory-associated protein GR and epigenetic modification enzyme HAT1, and reducing the expression of HDAC2 and the co-localization of HDAC2 protein and MeCP2 in the nucleus.
ABSTRACT
Objective To investigate the health education system for patients with fractures. Methods 400 cases of limb fracture patients admitted to hospital according to the date of the odd-even number of observation were randomly divided into two groups, control group was taken conventional health education. Observation group was given stage personalized health education system. That is,since the patient began to follow a systemafic,interactive,persenalized and the whole focus of the principle. Sub-phase of the hospital,the hospital phase,phase of the hospital, according to the patients and feedback in a timely manner to adjust the content and form of education. Education on the oral form of small seminars,one on the other a break and held a public forum,inehding treatment,care,nutrition,rest,attention to the disease,observation of the wound,placing the body parts and functions of the exercise. Results Compared with the control group observation groups' satisfaction and comfort to improve the conduct and compliance There was significant difference(P <0.01). Conclusion Systemic health education can enhance patient satisfaction and comfort,improve patient compliance,improve the health of patients with serf-awareness and ability ,reduce the incidence of complications,restore a clear role in the promotion in patients with fractures of the limbs.