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Objective:To evaluate the efficacy of acarbose combined with metformin in the treatment of type 2 diabetes with hyper-lipidemia. Methods:A retrospective study was adopted. A total of 2000 patients with type 2 diabetes and hyperlipidemia from general practice in the center were enrolled, and among them, 1050 patients were treated with acarbose, and the other 950 ones were treated with acarbose combined with metformin. After 3-month treatment, the therapeutic effect and adverse drug reactions were compared be-tween the groups. The changes of blood glucose and blood lipid were observed before and after the treatment. Results:The total effec-tive rate was 94. 83% in the combined group, which was significantly higher than that in the control group (72. 38%, P0. 05). Conclusion:Acar-bose combined with metformin in the treatment of type 2 diabetes with hyperlipidemia can significantly improve blood glucose levels and blood lipid levels, and the clinical efficacy should be affirmed.
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Aim TostudytheroleofASIC1aonthe matrix turnover and MAPK expression of the rat articu-lar chondrocytes with extracellular acidosis. Methods ArticularchondrocyteswereisolatedfromSprague-Dawley rats, and their phenotype was determined by toluidine blue and immunocytochemical staining. The GAG content of cell culture supernatant was deter-mined by dimethyl-methylene blue spectrophotometric assay, while Hyp content by chloramine T assay. ELISA assay was used to measure MMP-2 , TIMP-2 content. Furthermore, the ERK1/2, p38 MAPK phos-phorylation protein expression levels were tested by Westernblotassay.Results ASIC1acontributedto the effect of GAG, Hyp and TIMP-2 levels reduction induced by extracellular acidification, while the effect of MMP-2 was weaker. Moreover, ASIC1a could in-crease the ERK1/2 , p38 MAPK phosphorylation pro-teinexpressionlevels.Conclusion ASIC1acould regulate rat articular chondrocytes matrix turnover via ERK1/2 and p38 MAPK signaling pathway, and there-by inhibit the rat articular cartilage damage induced by acidosis.
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Objective To estabilish a RP-HPLC method for simultaneous determination of benzoic acid and salicylic acid in Compound Benzoic acid tincture.Methods ODSC18 chromatographic column made from Dalian Yilite(4.6mm × 150mm,5μm);mobile phase:methanol: water=60|: 40(pH adjust to 3.2);column temperature:30℃;detection wavelength:242nm;fiow rate:0.9ml/min.Results Ranges of linear of benzoic aci and salicylic acid were 30～120mg/L(R2=0.9995)and 15～60mg/L(R2=0.9988).The average recovery rate was 99.2％(RSD=0.95％)and 99.1％(RSD=1.36％).The RSDs of intra-day were 1.57％ and 1.57％(n=5).The BSDs of interday were 1.43％ and 1.59％(n=5).With t test,difference between Acid-base titration and RP-HPLC was not significant(P＞0.05).Conclusion The metold is simple,accurate and sensitive and can be used for the quality control of Compound Benzoic acid tincture.
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Objective The fingerprint method based on high-performance liquid chromatography for the quality control of bidens bipinnata L were firstly described and optimized. Methods HPLC system was used to obtain the fingerprint spectrum of bidens bipinnata L. The chromatography condition was ODS C18 column (4. 6mm×255mm,25μm),mobile phase Methanol-0. 2% HAc,and flow speed 1. 0ml ? min-1, detection wavelength 363nm.Results In this experiment,the standard fingerprint had been established and the similitude degree of 10 batches bidens bipinnata L. has also been obtained(0.941～0.999). Conclusion The method was accurate,reliable,good reproducibility, strong characteristic and could be used for the standard of general judgement and the quality estimation about the bidens bipinnata L.
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Objective: To measure the iodine nutritional status of students in Zhoushan Islands and evaluate the advantages and disadvantages of iodized salt prophylaxis. Methods: A comparative study was carried out in 12 classes which were selected from Dinghai(iodized salt district) and Daishan(non-iodized salt district) of Zhoushan Islands by random sampling. Mann-Whitney test was used to compare the urinary iodine concentration and dietary iodine intake of the two groups. Spearman correlation was used to look for the correlation between urinary iodine concentration and dietary iodine intake in the two groups. Ordinal regression was used to analyze the factors that affect the urinary iodine concentration. Poisson regression was used to analyze the risk factors of goiter.Results: The urinary iodine concentration in non-iodized salt district was 129 ?g/L,which was lower than 169 ?g/L in iodized salt district(u=6.302,P=0.000) , whereas the daily iodine intake in the two groups was 105 ?g/d and 184 ?g/d respectively, the difference was also significant(u=4.710,P=0.000). The influence of sex and iodized-salt intake was significant in the final regression model (P0.05). The effect of sex and iodized-salt intake on the development of goiter was significant in the Poisson model (P