Diagnostic value of HPV-DNA typing combined with serum NLR and DCLK1 for cervical cancer / 中国现代医生

@#Objective To investigate the value of receiver operating curve human papilloma virus(HPV)-DNA typing combined with serum neutrophil lymphocyte ratio(NLR)and bicorticoid kinase 1(DCLK1)levels in the early diagnosis of cervical cancer.Methods A total of 120 patients with early cervical cancer diagnosed in our obstetrics and gynecology department from August 2018 to June 2022 were randomly included as cervical cancer group,and 120 patients with benign lesions were included as benign group.The level of DCLK1 was detected by ELISA;NLR was detected by automatic blood cell analyzer;HPV subtypes in cervical secretions were detected by HPV genotyping gene chip detection system;the cut-off values of serum NLR and DCLK1 levels in the diagnosis of cervical cancer were analyzed by using the receiver operator curve(ROC);four table method was applied to analyze the diagnostic value of HPV-DNA typing,serum NLR,DCLK1 levels alone and in combination for cervical cancer.Results Compared with benign group,the levels of serum NLR and DCLK1 in cervical cancer group were obviously higher(P<0.05).The positive rate of HR-HPV in cervical cancer group was obviously higher than that in benign group(P<0.05).The ROC curve was drawn with serum NLR and DCLK1 levels as test variables,the results showed that the AUC of serum NLR and DCLK1 predicting early cervical cancer was 0.724 and 0.718,respectively,and the cut-off value was 3.08 and 3.32,respectively.HPV-DNA typing combined with serum NLR and DCLK1 detected 18 false positives and 17 false negatives,Kappa value was 0.725,which was consistent with pathological results.The sensitivity,negative predictive value and accuracy of HPV-DNA typing combined with serum NLR and DCLK1 levels in the diagnosis of early cervical cancer were obviously higher than those of HPV-DNA typing,serum NLR and DCLK1 levels alone(P<0.05).Conclusion The results of HPV-DNA typing combined with NLR and DCLK1 in the diagnosis of early cervical cancer are highly consistent with the pathological results,and the sensitivity and accuracy are obviously improved.

Chemical constituents contained in seeds of Notopterygium franchetii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the seeds of Notopterygium franchetii.</p><p><b>METHOD</b>Ethanol extracts of seeds N. franchetii were separated and purified by such methods as normal and reversed phase column chromatographies and thin-layer chromatography and structurally elucidated by MS and NMR evidences.</p><p><b>RESULT</b>Twenty nine compounds were separated, they were isoimperatorin (1), [3-sitosterol (2), phellopterin (3), bergapten (4), N-tetra, hexa, octacosanoylanthranilic acid (5-7), daucosterol (8), oxypeucedanin hydrate (9), umbelliferone (10), demethylfuropinnarin (11), (2S, 3S, 4R, 8E)-2-[(2'R)- 2'-hydroxydoco, trico, tetraco, entaco, hexaco sanosylamino] -octadecene-1, 3, 4-triol (12-16), (-)-oxypeucedanin (17), diosmetin (18), bergaptol-O-beta-D-glucopyranoside (19), nodakenin (20), 1'-O-beta-D-glucopyranosyl-(2R, 3S)-3-hydroxynodakenetin (21), uracil (22), decuroside V (23), 8-O-beta-D-glucopyranosyl-5-hydroxypsoralen (24), 8-O-beta-D-glucopyranosyl-5-methoxylpsoralen (25), diosmin (26), alaschanioside C (27), kynurenic acid (28) and mannitol (29).</p><p><b>CONCLUSION</b>All of these compounds were separated from the seeds of N. franchetii for the first time. Of them, 18, 22, 26 and 29 were firstly obtained from genus Notopterygium.</p>

Studies on chemical constituents from fruits of Forsythia suspense / 中国中药杂志

Twenty-four compounds in the fruits of Forsythia suspensa were isolated and purified by column chromatography and preparative TLC. On the basis of comprehensive spectroscopic methods including IR, ESI-MS/MS, 1D and 2D NMR, these compounds were identified as ten ceremides (1-10), six triterpenes (11-16), one steroids (17), three flavonoids (18-20), two C6-C2 alcohols (21-22) and two lignans (23-24). Compounds 1-10 were reported from F. suspense for the first time, among which 1, 2, 4 and 5 were new ones.

Chemical constituents of Incarvillea younghusbandii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Incarvillea younghusbandii.</p><p><b>METHOD</b>The chemical constituents were isolated by various column chromatographic methods and structurally identified by NMR and MS evidence.</p><p><b>RESULT</b>Fifteen compounds were obtained and identified as isobergapten (1), sphondin (2), imperatorin (3), xanthotoxin (4), phellopterin (5), heraclenol (6), rivulobirin A (7), methyl oleanolate (8), methyl caffeate (9), grevillic acid (10), boschniakinic acid (11), tert-O-beta-D-glucopyranosyl-(R)-heraclenol (12), 5-methoxy-8-O-beta-D-glucopyranosyloxypsoralen (13), 1'-O-beta-D-glucopyranosyl-3-hydroxynodakenetin (14) and phenylethyl-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside (15).</p><p><b>CONCLUSION</b>All of these compounds were isolated from this plant for the first time and most of them are furocoumarins.</p>

Cycloartane triterpenoid of Cimicifuga foetida / 中国中药杂志

<p><b>OBJECTIVE</b>To study cycloartane triterpenoid of Cimicifuga foetida and their osteoclast inhibition activity.</p><p><b>METHOD</b>The compounds were isolated and purified by normal and reversed phase column chromatographic methods. Structures were identified by spectroscopic analyses. Their osteoclast inhibition activity was then studied.</p><p><b>RESULT</b>Thirteen cycloartane triterpenoids were isolated from the rhizomes of C. foetida and were characterized as 25-O-acetyl-cimigenol (1), cimigenol (2), cimicidanol (3), 26-deoxyactein (4), asiaticoside A (5), 23-epi-26-deoxyactein (6), actein (7), 12beta-hydroxycimigenol (8), cimiside E (9), (23R, 24S) 25-O-acetyl-cimigenol-3-O-beta-D-xylopyranoside (10), (23R, 24S) cimigenol-3-O-beta-D-xylopyranoside (11), cimicifugoside H-1 (12) and cimicifugoside H-2 (13).</p><p><b>CONCLUSION</b>Compound 5 was isolated from this genus for the first time, compound 7 was isolated firstly from the plant and compound 2, 7, 10 and 11 exhibit osteoclast inhibition activity.</p>

Chemical constituents from root of Glycyrrhiza uralensis / 中草药

Object To investigate the chemical constituents from the roots of Glycyrrhiza uralensis Fisch. Methods The constituents were isolated on normal and reversed silica gel column chromatography and their structures were identified by spectral evidence. Results A new oleanane-type triterpenoid saponin and twelve known compounds, including two triterpenoid saponins, two cumarins and eight flavonoids, were isolated. Conclusion The new compound was elucidated as 3-O-[β-D-(6-methyl) glucuronopyranosyl (1→2)-D-glucuronopyranosyl]-24-hydroxy-glabrolide on the basis of ESI-MS, 1HNMR,13CNMR, HMQC and HMBC spectral evidence.

CHEMICAL CONSTITUENTS FROM THE ROOT OF RUBUS CHROOSEPALUS / 药学学报

AIM　To investigate the chemical constituents of Rubus chroosepalus Focke. METHODS The methanol extracts were suspended in H2O and then extracted with EtOAc. Column chromatography was used for separation and purification, while spectral analysis was used for identification. RESULTS　Seven compounds were isolated and identified as 2α,3β-dihydroxy-urs-12,19-dien-23,28-oic acid (I), 2α,3β,23-trihydroxy-urs-12,18-dien-28-oic acid (IIa), 2α,3β,23-trihydroxy-urs-12,19-dien-28-oic acid (IIb), 2α,3α-dihydroxy-urs-12,18-dien-28-oic acid (IIIa), 2α,3α-dihydroxy-urs-12,19-dien-28-oic acid (IIIb), and the acetonide of IIIa and IIIb (IVa and IVb). CONCLUSION　I was found to be a new pentacyclic triterpenoid acid.

Two new alkaloids from roots of Zanthoxylum nitidum / 中草药

Objective To study the chemical constituents from the roots of Zanthoxylum nitidum.Methods The constituents were isolated and purified by column chromatography.Their structures were identified on the basis of physiochemical properties and spectral data.Results Two benzophenanthridine alkaloids,1,3-bis(8-dihydronitidinyl)-acetone(1)and 8-acetonyldihydrofagaridine(2),have been iso-lated from the roots of Z.nitidum.Conclusion Compounds 1 and 2 are new compounds named nitidumtone A and nitidumtone B.

Studies on the Diterpenoid Alkaloids of Yiyulongwutou (Aconitum pseudostapfianum) / 中草药

From the roots of Aconitum pseudostapfianum W. T. Warig,ten diterpenoid alkaloids were isolated and identified as 14-benzoyl-8-O-methylaconine (Ⅰ ),aconitine(Ⅱ),deoxyaconitine(Ⅲ),penduline (Ⅳ),yunaconitine (Ⅴ), neolinine (Ⅵ), 15?-hydroxyneoline (Ⅶ), neoline (Ⅷ), talatisamine (Ⅸ)and aconosine (Ⅹ). I has not been previously found in nature.

Studies on chemical constituents from root of Rubus innominatus / 中草药

Object To study the chemical constituents from the root of Rubus innominatus S. Moore (Rosaceae). Methods Twelve compounds were isolated from the methanol extract by repeated column chromatography over normal and reversed phase silica gels. Their structures were identified by analysis of their spectral data and comparing TLC with authentic samples. Results Ten triterpenic acids and two steroids were isolated and identified. The antibacterial test indicated some triterpenic acids had antibacterial activity. Conclusion All compounds were obtained from the plants of Rubus L. for the first time.

Chemical constituents from root of Rubus irenaeus / 中草药

Object To investigate the chemical constituents from the methanol extract of the root of Rubus irenaeus Focke. Methods EtOAc extraction, normal and reverse phase silica gel column chromatography were used for isolation. Spectroscopic methods ( 13 CNMR , 1HNMR , DEPT, 2DNMR, and FAB-MS) and comparison with authentic samples were used for identification. Results Ten compounds were isoated and characterized as 2?, 19?-dihydroxyl-3-oxo-urs-12-en-28-oic acid (Ⅰ), 2-oxo-pomolic acid (Ⅱ), fupenzic acid (Ⅲ), euscaphic acid (Ⅳ), 2?, 3?, 19?-trihydroxyl-olean-12-en-28-oic acid (Ⅴ), 2, 3-O-isopropylidenyl euscaphic acid (Ⅵ), pomolic acid (Ⅶ), 2?, 3?-dihydroxyl-lup-20(29)-en-28-oic acid(Ⅷ), catechin (Ⅸ) and daucosterol (Ⅹ). Conclusion These compounds were obtained from this plant for the first time.

Chemical constituents of Notoseris gracilipes / 中草药

Object To investigate the chemical constituents from the whole plant of Notoseris gracilipes Shih Methods Compounds were isolated by various column chromatography and identified by spectral analysis Results Eight sesquiterpene lactones and four other compounds were isolated and identified as jacquilenin (Ⅰ), scorzoside (Ⅱ), ixerisoside D (Ⅲ), austricin (Ⅳ), notoserolide A (Ⅴ), crepidiaside A (Ⅵ), crepidiaside B (Ⅶ), and lactuside B (Ⅷ) The other four were 6, 7 dihydroxycoumarin (Ⅸ), cichoriin (Ⅹ), luteolin 7 glucoside (Ⅺ), and daucosterol ( ⅩⅡ ) Among them, compounds Ⅰ and Ⅱ showed antibacterial activity against Bacillu cereus AS 1 1688 Conclusion All compounds were isolated from this plant for the first time

Studies on chemical constituents of Swertia przewalskii / 中草药

Object To study the chemical constituents from the whole plant of Swertia przewalskii Pissjauk Methods The constituents were isolated on silica gel column chromatography and their structures were identified by spectral and chemical analysis Results Fifteen compounds were isolated and identified as 1, 7 dihydroxyl 3, 8 dimethoxyxanthone (Ⅰ), 1, 8 dihydroxyl 3, 7 dimethoxyxanthone (Ⅱ), 1, 7, 8 trihydroxyl 3 methoxyxanthone (Ⅲ), 1, 3, 7, 8 tetrahydroxyxanthone (Ⅳ), 1 O ? D glucopyranosyl 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅴ), 1 O ? D glucopyranosyl 8 hydroxyl 3, 7 dime thoxyxanthone (Ⅵ), 7 O ? D glucopyranosyl 1, 8 dihydroxyl 3 methoxyxanthone (Ⅶ), 1 O D xylo pyranosyl (1→6) ? D glucopranosyl] 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 O D xylopyranos yl (1→6) ? D glucopytanosyl] 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 O D xylopyanosyl (1→6) ? D glucopyranosyl] 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( Ⅹ 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( ⅩⅡ ), oleanolic acid ( ⅩⅢ ), ursolic acid ( ⅩⅣ ) and getiopicroside ( ⅩⅤ ), respectively Conclusion Compounds Ⅳ～Ⅷ, Ⅺ, ⅩⅡ , ⅩⅣ and ⅩⅤ were obtained from this plant for the first time