ABSTRACT
BACKGROUND: Studies have confirmed that monosialotetrahexosyl ganglioside can induce human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells, but little is reported on its optimal concentration. OBJECTIVE: To explore the optimal concentration of monosialotetrahexosyl ganglioside that induces human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells in vitro. METHODS: Human umbilical cord mesenchymal stem cells were isolated by using collagenase digestion method, and after expansion, passage 3 cells were randomly allocated into five groups. When 70%-80% of cells were confluent, 50, 100, 150 and 200 mg/L monosialotetrahexosyl ganglioside induction solutions were added in corresponding experimental groups, while cells in the blank control group were cultured in the same volume of L-DMEM medium. Cell morphology was observed under inverted phase contrast microscope. Expression levels of microtubule-associated protein 2, neurofilament protein and glial fibrillary acidic protein were measured by using immunohistochemistry at 6 hours after induction. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells were isolated successfully and sub-cultured stably. These cells could express surface markers of mesenchymal stem cells. Monosialotetrahexosyl ganglioside at the optimal concentration of 150 mg/L was confirmed to induce the neuron-like differentiation of human umbilical cord mesenchymal stem cells, and differentiated cells could express microtubule-associated protein 2 and neurofilament protein as neuron-specific markers.
ABSTRACT
Objective To investigate the level and influencing factors for self-efficacy among elders with hypertension in Zhoushan and to provide the reference for improving the self-efficacy of elders with hypertension. Methods The stratified sampling method was used to select elders with hypertension from 4 streets (townships) in urban and rural of Zhoushan in 2016. Four communities were selected from each street (townships) as sample units and using the random sampling to select 50 patients from every community. The investigation was performed with unified questionnaire. The influencing factors for self-efficacy were analyzed with logistic regression. Results The average score of the self-efficacy was 34.13±5.17. Among the investigated 738 elders with hypertension, more than half of them were scored 1ess than 2 in two items, which were blood pressure monitoring (86.99％) and persistent physical exercise (50.95％) . Scoring index of compliance behavior of hypertension was the lowest, only 63.93％ . By multivariate logistic regression analysis, those patients who were female (OR=2.34, 95％ CI: 1.55-3.53) , educated (ORprimary=1.72, 95％ CI: 1.09-2.69; ORjunior =2.25, 95％ CI: 1.13-4.46;ORsenior and above=2.46, 95％ CI: 1.06-5.71), with high level of drug compliance (ORmedium=1.72, 95％CI: 1.09-2.69; ORhigh=2.12, 95％ CI: 1.38-3.26) were more likely to get high level of self-efficacy. Patients who monitored blood pressure only uncomfortable were more likely to get low level of self-efficacy (OR=0.22, 95％ CI: 0.08-0.68) . Conclusion More than half of community-dwelling elders with hypertension had a middle level self-efficacy in Zhoushan, who need further improvement. Corresponding interventions should be developed to strengthen the self-efficacy, which can help patients improve self-management of hypertension and establish a healthy life style.
ABSTRACT
This article provides an overview of metastases to jaws (MJ), mainly concerning the differences between American and Chinese patients, and exploring the relationship between the primary tumors' prevalence (PTP) and constituent ratio of MJ. Information concerning of 399 MJ cases in 215 papers, including one new case in our hospital, was subjected to statistic analysis. The main clinical features of MJ, such as constituent ratio of PTP and that of MJ, metastatic sites, treatments, and prognosis were summarized. Breast, lung, kidney, prostate and thyroid (in descending order) were the leading primary sites of MJ. Furthermore, the constituent ratio of MJ was found to be correlated with that of PTP in all subjects including American and Chinese subjects in our study. As to metastatic sites in the mandible, a specific "M" shaped pattern appeared regardless of the tumor type or constituent ratios of MJ were in all subjects. Almost all subjects received traditionally palliative treatments, and the prognosis was quite poor. The PTP had a significant impact on the constituent ratio of MJ. However, it was the properties of the microenvironment rather than characteristics or constituent ratios of tumor cells, that decided the metastatic sites in various tumor subjects.