ABSTRACT
It is known that vitreous humor in rabbit is made of many components including, hyaluronic acid, collagen, hyalocytes, inorganic salts, sugars, ascorbic acid, and water. Based on the fact that hyaluronic acid is a biostimulator, in current project we tried to elucidate the effect of vitreous humor extracts on wound repair and tissue regeneration in rabbits. Ears of several white Newzealand rabbits [n=6], about 2.5kg each, were locally anaesthetized with lidocaine spray solution, and punched-hole manually with a special puncher. Three circular punches whit 8mm in diameter were made in each ear, and used for control or test groups. Daily we applied vitreous humor extracts on the test group wounds, the size of each treated wound was measured carefully and compared with control groups every 3 days, and finally the data representing the ratio between regenerated and non-regenerated tissues of proximal, medial and distal positions were collected.Histograms of macrosopic studies showed that treating of wounds by vitreous humor has obvious effect in their repair. Statistical analysis also showed a significant difference [p<0.05] between mean value of regeneration in test and control groups. Microscopic data showed that in the test groups there was also a significant increase in the following phenomena: 1] in epidermic thickness and presence of dermal papilla, resulting in outgrowth of epidermis, 2] in the formation of fresh fibroblasts and connective tissues, 3] in angiogenesis and hair follicle formation.In conclusion these data showed that rabbit vitreous humor potentially is a good candidate to improve wound repair and promote tissue regeneration. This extract may exert its effects due to presence of a biostimulator, hyaluronic acid, in its components