ABSTRACT
Background and Objective: Yersinia is a gram-negative bacillus that cause diarrhea through consumption of contaminated food and water. This study was performed to identify the atypical Yersinia virulence markers isolated from children with diarrhea
Methods: This descriptive cross -sectional study was done on 384 fecal samples of 0- 14 years old children admitted at children medical center from August 2011 to August of 2012. Fecal samples, for the enrichment, after 21 days of incubation in alkaline buffer with pH=7.2 at 4degree C, on days 7,14 and 21 samples were cultured on CIN agar and Mac agar and then confirm the differentiation atypical Yersinia from other typical Yersinia species from fermentation of different sugars. Isolates were tested for marker of virulence including calcium dependence, auto agglutination, Congo red uptake and binding of crystal violet
Results: Out of 384 stool samples, 4 [1.04%] were infected with Yersinia [Yersinia frederikseni, Yersinia kristensenii and Yersinia enterocolitica]
Out of these three, only two samples in association was positive with virulence markers
Conclusion: Phenotypic markers can be used to study the properties of phenotypic strains of Yersinia
ABSTRACT
FFP [Fresh Frozen Plasma] containing all coagulation factors is used to treat coagulation disorders. In the present study, FFP consumption in Children Medical Center was studied to evaluate transfusion indications leading to blood orders some of which might have been unnecessary bringing about inappropriate blood use or unused blood donations. In this retrospective study, 1262 FFP order and reservation requests during one year for being administered to 735 patients were reviewed by census method. The results were analyzed by Chi square and SPSS. Out of 1262 requested FFP units, 952 [75.4%] were transfused, and 90 units [7.13%] though thawed were not. Out of 220 reserved units, only 22 units were transfused. Taking more attention in ordering will prevent blood loss and increase economic saving. The blood bank of Children Medical Center does not embark on thawing FFP before its usage is finally confirmed so that blood loss is somehow avoided
Subject(s)
Humans , Child , Retrospective StudiesABSTRACT
Urinary tract infection [UTI] is considered as the most common bacterial infectious disease seen among the pediatric patients. The aim of this study was to investigate the prevalence and antimicrobial susceptibility of bacterial uropathogens isolated from the pediatric patients with urinary tract infections. This descriptive study was conducted in Children Medical Center, Tehran, Iran from March 2006 to Feb 2007. Clean-catch midstream urine specimens were obtained from the patients and cultured on the appropriate bacteriological media. Bacterial isolates were identified by standard biochemical and serological tests. Antimicrobial susceptibility testing was performed according to CLSI guidelines. From 14199 urine specimens, 16.2% had positive results for bacterial cultures. Nine hundred twenty one strains were identified as Escherichia coli; 412 as Klebsiella spp., 285 as Coagulase negative Staphylocococci, 202 as Enterococcus spp., 158 as Pseudomonas spp., and 83 as Staphylococcus aureus. E. coli isolates showed high resistance to carbenicillin [68%], ampicillin [96%], trimethoprim-sulfomethoxazol [70%] and kanamycin [65%]. More than 30% of isolates of Klebsiella spp., Pseudomonas spp. and Enterobacter spp. have shown high degree of resistance to commonly used antibiotics. Our findings reinforce the need for ongoing investigation to show trends in antibiotic resistance, which can help to prescribing of antibiotics in clinics