ABSTRACT
To investigate the prevalence of PER-1, PER-2, GES, IMP-1, VIM-2, OXA-23, and OXA-24 type beta-lactamases in carbapenem-resistant Acinetobacter baumannii isolates, in view of the fact that Beta-lactamase production is the most important mechanism of acquired beta-lactam resistance in Gram-negative pathogens. Experimental study. Department of Medical Microbiology, School of Medicine, Pamukkale University, Denizli, Turkey. From January 2009 to December 2009, a total of 98 carbapenem-resistant A. baumannii bacteria were isolated from patients. In-house PCR was performed for the detection of PER-1, PER-2, GES, IMP-1, VIM-2, OXA-23, and OXA-24 beta-lactamase genes in those clinical carbapenem-resistant A. baumannii isolates. Prevalence rate of beta-lactamase genes in carbapenem-resistant A. baumannii isolates Results: GES beta-lactamase was found in 59 isolates [60.20%]. PER-1 was found in eight isolates [8.16%]. IMP-1 was found in five isolates [5.10%]. OXA-24 was detected in two isolates [2.04%]. No isolate possessed VIM-2, PER-2, or OXA-23 beta-lactamase genes. This study indicates that the GES type beta-lactamases are the most prevalent among carbapenem-resistant A. baumannii isolates in our hospital. Screening for beta-lactamases and strict infection control for these isolates will help prevent further spread of resistance
ABSTRACT
Objectives: To investigate the seroprevalence of anti-hepatitis E virus antibody among primary school children in the two different areas of Denizli, Turkey
Methodology: Anti-HEV antibodies were investigated in 185 primary school children [91 from rural areas and 94 from urban areas of Denizli]. The children were divided into two age groups as seven-year old group and fourteen-year old group. Samples were tested for anti-HEV Ab by an enzyme-linked immunoassay
Results: A total of 23 primary school children were anti-HEV Ab positive, giving a prevalence of 12.4%. The seroprevalence rate was 13.1% in rural areas and 11.7% in urban areas. The difference in the seropositive rates was not statistically significant [p > 0.05]. Among 185 primary school children, Anti-HEV antibodies were positive 17 [18.1%] in seven-year old group, and 6 [6.6%] in fourteen-year old group. The difference in the seropositive rates was statistically significant [p < 0.05]
Conclusions: There was no association between the anti-HEV Ab and gender, socioeconomic level, parental educational level, rural or urban areas. Anti-HEV Ab seroprevalence was higher in seven-year old children than fourteen-year old children