ABSTRACT
OBJECTIVE@#To explore the effect of Fuzhengyangying granules (FZYYG) on the expression of the bone marrow proliferation and bcl-2 in mice with immune mediated aplastic anemia.@*METHODS@#Forty BALB/c mice were randomly allocated to 4 groups: a normal control group, a model group, cyclosporine A (CSA) group and FZYYG group. The model group was fed with physical salt, while the CSA and FZYYG group were fed with CSA and FZYYG respectively, and then the changes of peripheral hemoglobin (Hb), platelet, bone marrow nucleus cell (BMNC), bone marrow hematopoiesis tissue volume and bcl-2 expression were examined.@*RESULTS@#The count of Hb, the platelet, BMNC, the bone marrow hematopoiesis tissue volume and the bcl-2 positive rate in the model group were lower than those in the normal group (P<0.05), whereas the count of Hb, the platelet, BMNC, the bone marrow hematopoiesis tissue volume and the bcl-2 positive rate in both CAS and FZYYG groups were significantly higher than those in the model group (P<0.05).@*CONCLUSION@#FZYYG can induce the bcl-2 antigen expression and postpone the haematogenous cells apoptosis, and it is effective for mice with immune mediated aplastic anemia.
Subject(s)
Animals , Female , Male , Mice , Anemia, Aplastic , Blood , Allergy and Immunology , Bone Marrow Cells , Metabolism , Pathology , Cell Proliferation , Drugs, Chinese Herbal , Pharmacology , Immunohistochemistry , Mice, Inbred BALB C , Mice, Inbred DBA , Proto-Oncogene Proteins c-bcl-2 , Random AllocationABSTRACT
OBJECTIVE@#To explore the effect of Naoxintong capsules on learning and dysmnesia and calcitonin gene-related peptide (CGRP) about vascular dementia (VD) in rats.@*METHODS@#The model of vascular dementia rats were built by middle cerebral artery occlusion (MCAO), and then the rats were randomly divided into a Naoxintong group (that was traditional Chinese medicine group,TCM group), a Western medicine group, a model group, a sham operation group, and a normal group. After corresponding treatment, the rat so behaviors were detected by Morris water maze experiment to obtain their performance record of learning and memory. The changes of cell morphology were detected by Nissl stain. The expression of CGRP in the hippocampus was measured with immunohistochemical staining.@*RESULTS@#The masculine cells about CGRP of the TCM group were more than those of the model one (P<0.05). Naoxintong capsules improved the ability of learning and memory in vascular dementia rats, prevented the pyramidal cells on CA1 field in hippocampus from decreasing, increased the number of masculine cells about CGRP, and deceased the gray scale value.@*CONCLUSION@#Naoxintong capsules can raise the number of masculine nerve cells of CGRP, protect the pyramidal cells of VD rats, and lessen their learning and dysmnesia, which may be part of the mechanism of improving learning and memory capacity of VD model in rats.
Subject(s)
Animals , Female , Male , Rats , Calcitonin Gene-Related Peptide , Metabolism , Dementia, Vascular , Metabolism , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Hippocampus , Cell Biology , Metabolism , Maze Learning , Pyramidal Cells , Metabolism , Rats, Sprague-DawleyABSTRACT
OBJECTIVE@#To observe the effect of naoling decoction (NLD) on the behavior and the mRNA expression of beta-amyloid precursor protein (APP) in the hippocampus in rat model with Alzheimer's disease (AD).@*METHODS@#D-galactose was intra-abdominally injected and AlCl3was hypodermically injected to build the AD models. The behavior of rats was measured by gamma-electric maze and the level of APP mRNA in the model rat hippocampus was observed by RT-PCR.@*RESULTS@#The learning and memory capacity in the NLD group was improved and the APP mRNA expression level was significantly lower in the NLD group compared with the untreated model and the control group (All P < 0.05).@*CONCLUSION@#NLD can lower the expression of APP mRNA to reduce beta-amyloid protein deposition of rat hippocampus, which may be one of the mechanisms of improving the learning and memory capacity of AD model rats.
Subject(s)
Animals , Female , Male , Rats , Alzheimer Disease , Metabolism , Amyloid beta-Protein Precursor , Genetics , Behavior, Animal , Drugs, Chinese Herbal , Pharmacology , Hippocampus , Metabolism , Learning , Memory , RNA, Messenger , Genetics , Random Allocation , Rats, Sprague-DawleyABSTRACT
OBJECTIVE@#To observe the effect of fuzheng yangying oral solution on bone marrow proliferation and serum interleukin 2 in immune-induced aplastic anemia mice.@*METHODS@#The immune-induced aplastic anemia mouse models were built up, and then were divided into 4 groups by randomized allocation. The models were fed with physical salts (Group A, n = 10 ), fuzheng yangying oral solution (Group B, n = 10), cyclosporin A (Group C, n = 10), and fuzheng yangying oral solution plus cyclosporin A (Group D, n =10), respectively. Another 10 healthy mice served as controls (Group E). The changes in blood cells, bone marrow karyocyte count, marrow hematopoietic tissue capacity, and serum interleukin 2 level were observed.@*RESULTS@#The blood cells, bone marrow karyocyte count, and marrow hematopoietic tissue capacity in Group A were significantly lower than those of Group E (P 0. 05 ). The level of interleukin 2 in Group A was obviously higher than that of Group E (P < 0. 05 ); The levels of interleukin 2 in the 3 treated group ( Group B, C, and D) were significantly lower than those of Group A ( P < 0. 05 ); and Group D was the lowest ( P < 0. 05). There was no significant difference in the level of interleukin 2 between Group B and C ( P < 0. 05).@*CONCLUSION@#Fuzeng yangying oral solution can impove the bone marrow proliferation and reduce the level of serum interleukin 2 in immune-induced aplastic anemia mice.