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Objective To explore the diagnostic value of cfDNA in pancreatic cancer. Methods A total of 467 patients with pancreatic cancer and 129 healthy controls were enrolled. cfDNA concentration was detected using the QuantiDNA Direct cfDNA Test (DiaCarta) kit. Mann-Whitney U test was used to compare cfDNA concentration between different groups, and Chi-square test was used to analyze the relationship between cfDNA and pathological data of pancreatic cancer. Diagnostic efficacy was evaluated by ROC analysis. Results The cfDNA level of patients with pancreatic cancer was significantly higher than that of healthy controls (20.85 vs. 15.15 ng/ml, P=0.0027). The positive rate of cfDNA in pancreatic cancer was higher than that in healthy controls (77.73% vs. 59.68%). The sensitivity levels of cfDNA, CEA, and CA19-9 as diagnostic markers were 62.65%, 64.04%, and 60.32%, and their specificity levels were 61.05%, 70.53%, and 87.37%, respectively. The sensitivity and specificity of the combined detection were 77.26% and 53.58% respectively. The areas under the curve of cfDNA, CEA, CA199, and combined detection were 0.62, 0.67, 0.74, and 0.67, respectively. Conclusion cfDNA is elevated in patients with pancreatic cancer and may serve as an effective marker for its diagnosis.
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Objective To investigate the level of plasma MALAT1 in breast cancer(BC)patients and its clinical significance.Methods The expression levels of MALAT1 different fragments were detected in plasma from 10 healthy controls.The expressions of GAPDH and MALAT1 of plasma samples collected from 102 preoperative breast cancer patients,64 postoperative breast cancer patients,47 breast benign tumor patients and 50 healthy controls were determined by RT-qPCR.The potential association between plasma GAPDH level and cases′clinicopathologic features was analyzed to evaluate the stability of GAPDH. Receiver operating characteristic(ROC)curve was constructed to evaluate the diagnostic efficiency of MALAT1,CA153 and CEA for breast cancer.Meanwhile, the association between MALAT1 level and the clinicopathologic features and the expressions of MALAT 1 between preoperative and postoperative BC patients were analyzed.T-test and one-factor ANOVA test were used for normal distribution of quantitative data.The rank sum test was used for non-normal distribution of data.Results GAPDH level was stable in female plasma and was not affected by age and pathology(P>0.05).GAPDH can be used as a reference for plasma lncRNA detection.The levels of different MALAT1 fragments were inconsistent(χ2=27.042,P<0.001).Levels of MALAT1 were significantly elevated in preoperative BC patients[5.58(2.17-12.34)] compared with breast benign tumor patients and healthy controls[1.08(0.61 -2.58)(Z=6.209,P<0.001),1.63(0.98 -3.51)(Z=4.871,P<0.001)].However, there was no significantly difference between breast benign tumor patients and healthy controls(Z=-1.675,P=0.094).The MALAT1 levels of low grade patients(gradeI and II)were higher than those of breast benign tumor patients(Z=5.593,P<0.001).The relative expression of MALAT1 in postoperative plasma was significantly reduced(Z=-2.248,P=0.025).Areas under the ROC curve of MALAT1,CA153 and CEA were 0.744,0.619 and 0.553 respectively.The sensitivity and specificity were 54.1%,60.0%,70.0% and 86.3%,66.7%, 44.1%respectively.The levels of MALAT1 were associated with TNM stage(Z=-1.982,P=0.047), lymph node metastasis(Z=-2.186,P=0.029)and tumor differentiation(Z=-2.435,P=0.015). Conclusion The expressions of MALAT1 were highly elevated in BC patients.Plasma MALAT1 may be an important biomarker for the diagnosis of breast cancer.
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Objective To explore the relative expression of plasma miR-199a-5p and miR-200c-3p in gastric adenocarcinoma cancer(GAC) patients and its clinical value.Methods Case-control study was used in this research.The relative expression of plasma miR-199a-5p and miR-200c-3p from 47 GAC patients and 50 healthy controls were determined by RT-PCR ( TaqMan Probe method).Meanwhile, the association with age, gender, tumor location, size, degree of differentiation, TNM stage, lymph node metastasis and other clinical pathological parameters were analyzed.The expression of these two miRNAs in plasma of 30 GAC patients during preoperation was compared with their expression 6-8 days after radical surgery.The sensitivity and specificity of plasma miRNAs expression for the diagnosis of GAC were analyzed using the receiver operating characteristic ( ROC ) curve.SPSS20.0 statistical software was used for statistical analysis.T-test, paired t-test and one-factor ANOVA were used for normal distribution of quantitative data.Results The plasma level of miR-199a-5p in GAC patients was significantly lower(1.05 ±0.22) (t =3.058,P =0.003), while miR-200c-3p was significantly higher(15.15 ±3.02) (t =-2.854,P=0.006), when they were compared with those in controls(26.80 ±8.38, 3.39 ±0.87).Low miR-199a-5p expression in GAC patients were associated with lymph node metastasis ( F =4.725, P =0.029) and the differentiation degree of gastric cancer(F=3.854,P=0.032).The relative expression of miR-199a-5p in postoperative plasma was significantly increased(t=-3.814,P=0.001), but the relative expression of miR-200c-3p was significantly reduced when compared to the preoperative samples(t=2.978, P=0.006).Area under the ROC curve of miR-199a-5p, miR-200c-3p and combined miR-199a-5p and miR-200c-3p were 0.692, 0.792 and 0.798, the sensitivity and specificity were 87%,97%,92.5% and 43%,54%, 65%, respectively.Conclusion Combined detection of miR-199a-5p and miR-200c-3p in plasma has a higher sensitivity and specificity than the conventional tumor marker CEA and CA19-9, and may be a useful combination for gastric cancer diagnosis.
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Objective To systematically review the diagnostic value of microRNA(miRNA) quantitation in breast cancer .Meth-ods Literatures about miRNA and breast cancer diagnosis were selected by retrieving Medline ,Embase and Cochrane Library .Ac-cording to the inclusion and exclusion criteria ,the literatures were independently screened ,and a 2 × 2 contingency table was con-structed .Quality of literatures was assessed by quality assessment of diagnostic accuracy studies(QUADAS) .Statistical analysis was performed by employing Meta-Disc 1 .4 software and STATA 11 .0 .Results 16 studies were included ,which contained 1 303 patients and 711 control samples .There were threshold effects among these studies (the spearman′s correlation coefficient was-0 .758 ,P=0 .001) .A random effects model was used for meta-analysis .The summary sensitivity ,specificity ,positive likelihood ratio ,negative likelihood ratio ,and diagnostic odds ratio for miRNA in breast cancer diagnosis were 0 .77(95% CI:0 .75 -0 .79) , 0 .77(95% CI:0 .74-0 .80) ,4 .19(95% CI:2 .79-6 .30) ,0 .25(95% CI:0 .19-0 .35) ,19 .91(95% CI:9 .68-40 .95) .The area un-der curve of SROC was 0 .895 0 .Conclusion These results suggest that miRNAs have potential value to diagnose breast cancer . However ,effective diagnosis of breast cancer still needs to be conducted with assistance of clinical findings and traditional lab inves-tigations .