ABSTRACT
Objective: To find out serum zinc level in healthy pregnant women and pre-eclemptic pregnant women
Study Design: Ramdomized controlled trial study
Place and Duration of Study: This study was conducted at Department of Pathology, Lady Willingdon Hospital, Lahore and at Lahore College for Women University, Lahore from March 2015 to September 2015
Materials and Methods: Size of sample was determined statistically by using table and119 [102 experimental group[51 pre-eclamptic pregnant women and 51 normal pregnant women] and 17 control group] blood samples were collected randomly
Results: The average zinc concentration in healthy pregnant group was found 1.44 +/- 0.14 mg/l as compared to pre- eclamptic pregnant group 0.25 +/- 0.02 mg/l. In pregnant women average zinc concentration was found to be decreasing with trimester. The average systolic blood pressure in healthy pregnant women was 113.83 +/- 1.74 mm/Hg as compared to the pre-eclamptic pregnant women was 145.34 +/- 1.68 mm/Hg. Average diastolic blood pressure in healthy pregnant women, was found 75.23 +/- 1.46 mm/Hg as compared to pre-eclamptic pregnant women, which was 92.76 +/- 1.80 mm/Hg
Conclusion: Zinc level in the blood serum of pre-eclamptic pregnant women was found lower as compared to healthy pregnant women. Low blood serum zinc level is associated with the elevated systolic and diastolic blood pressure also
ABSTRACT
The current study was carried out in order to assess the toxic effects enforced by nicotine on liver, lung, and testes of mice. Experimental study. This study was conducted at the Animal House of Lahore College for Women University Lahore for a period of thirty days. The initial body weights of mice were obtained. A control group consisting of 20 mice injected with saline solution and an experimental group containing 40 mice treated with 1 mg/kg of nicotine subcutaneously were designed. The treatment lasted for 6 weeks after which final body weights were recorded. Later the mice of both groups were slaughtered and their livers, lungs, and testes were taken out and directly preserved in 10% formalin. A considerable decrease of body weight and food intake was evident in the experimental group and so was observed in the organs weight too. The decrease of body weight and food intake was from 36.89 +/- 1.31 to [35.39 +/- 1.25] and [119.41 +/- 5.76] to [115.01 +/- 5.50] respectively. All the three organs also showed a prominent decrease of the weight and resulted in the degeneration and alteration of the histology. Major histological changes in liver were widening and enlargement of sinusoids, necrosis, degeneration of hepatocytes, and fat deposition. Testes had disruptions in the seminiferous tubules and less number of Leydig cells, and experimental lungs showed proliferation of cells, damaged connective tissue network and congestion of lungs. Nicotine administration to the encountered animals reduces the body weight. Decrease in body weight is considered to be due to reduction in food intake. By examining the liver, lungs, and testes affected by nicotine, it can be figured out that nicotine greatly affects the histoarchitecture of the three organs in several ways. For functional integrity of the organs, extreme and direct exposure to such drugs must be prevented
ABSTRACT
Glucocorticoids [GC] actuate apoptosis as well as cell cycle arrest in lymphocytes, and included as core element in the lymphoid malignancy treatment. Despite clinical significance of GC and considerable efforts to understand it, the molecular basis of GC regulated cell death and the resistance phenomenon remains, however, poorly understood. Using Affymetrix-based whole genome expression profiling our group has previously identified a number of prominent glucocorticoid-response genes [Blood 107: 2061, 2006]. Promyelocytic leukemia zinc finger [PLZF] was one of the best candidate genes. This study was proposed to investigate the possible role of PLZF in GC regulated cell death in leukemic model cell line NALM6. To this end, we generated NALM6 cell line [bulk] transduced with a retroviral expression vectors, pHR-SFFV-PLZF-IRES-Puro [U426] and pHR-SFFV-Venus-IRES-Puro [U417], as control, for constitutive gene-expression. HEK293T cells were transfected transiently to generate viral particles. These cell lines were characterized by Western blotting and used to assay the effect of constitutive PLZF expression. In conclusion, we report that bona fide transcription repressor PLZF, which turned out as prominent GC-regulated gene both in vivo and in vitro situations was found to enhance the GC-induced cell death [basal] in leukemic model cell line NALM6 after 48 and 72h time points
Subject(s)
Humans , Leukemia/drug therapy , Glucocorticoids/pharmacology , Kruppel-Like Transcription Factors/physiology , Cell Line, Tumor , HEK293 CellsABSTRACT
Hydatidosis is a zoonotic public health problem globally and in Pakistan also causing illness in human and animals. The purpose of this study was to determine the prevalence of disease. None randomized prospective study. This study was conducted in different abattoirs of Pujab [Faisalabad and Lahore], Pakistan from 2004-2008. In present study 39738 male and female animals [sheep, goats, buffaloes, cattle and camels] were examined. Liver and Lungs were main visceral organs with bigger size of cyst collected and processed on Real Time PCR. Highest prevalence [%] was found 7.29 [102/590] in camels and lowest 5.18 [155/2990] in cattle. Prevalence of fertile cysts was also determined and found highest [95%] in camels and lowest [75%] in cattle. Sterile, calcified and under developed cysts were also seen but without any significant number, except in cattle sterile cysts were significantly high [P<0.05]. Study showed high prevalence in two [Faisalabad and Lahore] out of six abattoirs visited in different big cites. It is concluded that in Punjab hydatidosis is significantly prevalence. The major cause of this prevalence is improper disposal of infected organs and unhygienic conditions of abattoirs