Evaluation of capillaria philippinensis coproantigen in the diagnosis of infection

In order to overcome the false negative diagnosis of infection with C. philippinensis in the absence of eggs in stool, coproantigen prepared from the stools of infected patients was evaluated serologically. This antigen was able to detect anti-Capillaria antibodies in the sera of infected cases at the same OD level produced with Capillaria crude worm antigen using indirect ELISA technique. C. Philippinensis coproantigen did not cross-react with the sera from patients with Schistosomiasis mansoni, Fascioliasis or Strongyloidiasis at 1:00 serum dilution. Laboratory-prepared hyperimmune sera versus crude worm antigen of C. philippinensis succeeded in capturing Capillaria antigen prepared from the stools of infected patients and did not cross-react with the coproantigens prepared from the stool samples of cases infected with S. Mansoni or Fasciola using sandwich ELISA technique

Intestinal capillariasis in Egypt epidemiologigal background

Forty-four cases of intestinal capillariasis were diagnosed; 37 females and 7 males with ages ranged from 10 to 65 years, however most of them were between 20-40 years. Twenty-six cases were from the northern part of Upper Egypt, particularly Beni-Swif and Menia governorates. The patients were presented with borborygmi, chronic diarrhea, vomiting, loss of weight, lower limb edema and electrolyte imbalance for durations ranging from one month to two years. The morbidity and mortality of the disease in Egyptian cases were described. Some patients gave history of eating raw whole fish, while others gave history of eating raw parts of fish or half-cooked fish. The diagnosis was based on the clinical picture and confirmed stool analysis. The differential diagnosis was discussed and the cost paid by patients to reach the correct diagnosis was estimated

Parasitological aspects of capillaria philippinensis recovered from egyptian patients

Seven new cases of intestinal capillariasis were detected during the period from July 1997 to June 1998. A parasitological study concerning the morphological features of Capillaria philippinensis recovered from the stool of Egyptian cases was carried out. Eggs were separated, cultured and used to infect Oreochromis nilotica niloticus, a famous popularly consumed type of fresh water fish in Egypt in a trial for identifying the fish intermediate host for hits parasite, but infection had not developed in the intestine of this species of fish

Study on humoral and lymphocyte specific reactions in patients infected with Schistosoma mansoni

This study was carried out on 25 patients [10 males and 15 females]. Their ages ranged between 8 and 45 years. The control group included 8 healthy subjects [3 females and 5 males] free of schistosomiasis and also from other parasitic diseases. Their ages ranged between 14 and 35 years. All cases were subjected to stool analysis by direct smear method, simple sedimentation technique and egg counting by modified Kato technique. The detection of immune response by IHAT [indirect haemagglutination test], colorimetric technique using MTT [Dimethyl tetrazolium salt] was used to detect lymphoblastic reaction to SEA [schistosoma egg antigen] and to PHA mitogen [phytohaemagglutinin]. The variation in stool egg counts showed insignificant difference in relation to age, IgG titre [IHAT] and lymphoblastic response to SEA and PHA among the patients group. IgG titer was found moderately high in the patients groups. The non specific lymphoblastic response to PHA was significantly higher in control group versus infected group [P < 0.001]. The lymphoblastic reaction to SEA was significantly higher in patients compared to control group [P < 0.001]