ABSTRACT
The present study was carried out to evaluate the potential of adipose-derived mesenchymal stem cell (AD-MSCs) to enhance the rate of healing of full-thickness excisional skin wounds in rabbits. Six healthy adult New Zealand white rabbits and five healthy Swiss Albino mice were used for the study. Two, 2 × 2 cm full-thickness skin (thoracolumabar region) excisional wounds were created; one on each side of the dorsal midline in each animal. Adipose tissue was collected from the abdomen of the mice and processed for isolation of AD-MSCs. The wounds were randomly assigned to either injection of adipose-derived mesenchymal stem cell into the wound margins (AD-MSCs), or topical application of Povidone iodine (5%) solution (PI) as positive control. The wound healing was assessed by evaluation of granulation tissue formation, epithelisation and histomorphological study on 7th, 14th, 21st and 28th postoperative days. Better epithelisation was seen histologically in AD-MSCs treated wounds than in PI-treated wounds. Histomorphological examination of the healing tissue showed early disappearance of inflammatory reaction, significantly more neovascularisation, and more fibroplasias and early lay down and histological maturation of collagen in AD-MSCs treated wounds than in PI treated wounds. Hence the application of xenogenic stem cells can be used for tissue engineering and regenerative medicine in animals
ABSTRACT
Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person who needs to do section cutting. In the routine method of preparing paraffin sections, it is often encountered by the presence of artefacts like fine wrinkles or folds. Attempts have been made to remove the wrinkles by floating the sections in the warm water bath. However this method has not been able to remove all the wrinkles from the sections. We have designed a simple and reliable method, in which the paraffin sections were floated over the ethyl alcohol diluted with water (1:15) before they were placed in the water bath. Through this method, we have been able to get the wrinkle free sections of superior quality. The advantage of our method is that, it is easy to prepare the dilute alcohol and is cost effective. This method can be used by the histology and pathology technicians and the researchers.
Histotecnología se refiere a la elaboración y preparación de los tejidos del cuerpo de tal forma que permitan un estudio satisfactorio de éste. El corte seccionado es una parte integral de la histología y la histopatología. Es un arte por sí mismo que requiere habilidad y conocimiento por parte del técnico o la persona que tiene que hacer el corte de la sección. En el método de rutina de la preparación de las secciones de parafina se encuentra a menudo la presencia de artefactos como arrugas finas o pliegues. Se han hecho intentos para eliminar las arrugas por flotación de las secciones en el baño de agua tibia. Sin embargo, este método no ha sido capaz de eliminar todas las arrugas de las secciones. Hemos diseñado un método sencillo y fiable, en el que las secciones de parafina fueron colocadas por flotación sobre alcohol etílico diluido con agua (1:15) antes de ser colocado en el baño de agua. A través de este método, hemos sido capaces de obtener secciones sin arrugas de calidad superior. La ventaja de nuestro método es que, es fácil de preparar el alcohol diluido y es rentable. Este método puede ser utilizado por los técnicos de histología, patología e investigadores.