1.
Indian J Biochem Biophys
; 2015 Feb; 52 (1): 107-110
Article
in English
| IMSEAR
| ID: sea-157962
ABSTRACT
Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.