ABSTRACT
Background: Metallo-ß-lactamases (MBL) confer high resistance to carbapenems in Pseudomonas aeruginosa (Psae). They are encoded in mobile elements of different genes (VIM, IMP, SMP, GIM), along with other resistance genes. Aim: To detect the presence of MBL in imipenem resistant Psae strains. Material and methods: Fifty-nine imipenem resistant Psae strains isolated from January 2004 to August 2005 in a University Clinical Hospital, were included. The presence of MBL was studied by Etest (phenotypic) and genotypic polymerase chain reaction (PCR) methods. To rule out a nosocomial outbreak, MBL positive strains, were studied by pulse field gel electrophoresis. Results: The presente of MBL was detected in eleven strains. AH were type VIM and were not clonally related. There was no concordance between phenotypic and genotypic MBL detecting methods. AH the strains were also multiresistant. Conclusions: The presence of MBL was detected in 19 percent of imipenem resistant Psae strains.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Anti-Bacterial Agents/pharmacology , Imipenem/pharmacology , Pseudomonas Infections/drug therapy , Pseudomonas aeruginosa/enzymology , beta-Lactamases/genetics , Cross Infection/epidemiology , Cross Infection/genetics , Drug Resistance, Multiple, Bacterial/drug effects , Drug Resistance, Multiple, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Genes, Bacterial/drug effects , Genes, Bacterial/genetics , Imipenem/analysis , Microbial Sensitivity Tests , Polymerase Chain Reaction , Pseudomonas Infections/genetics , Pseudomonas aeruginosa/drug effects , Young Adult , beta-Lactam Resistance/drug effects , beta-Lactam Resistance/genetics , beta-Lactamases/analysisABSTRACT
Introducción: La actividad de la enzima convertidora de angiotensina I (ECA) está determinada importantemente por un polimorfismo de la misma enzima. Las ratas Brown Norway (BB) tienen mayor actividad de ECA (y niveles de angiotensina II) que ratas Lewis (LL). Debido a que Ang II induce la activación de NADPH oxidasa, se postula que el polimorfismo BB determina mayor actividad de NADPH oxidasa y mayor producción de anión superóxido (O2). Métodos: Se usaron ratas homocigotas BB y LL, que tienen niveles altos y bajos de ECA, respectivamente. La actividad enzimática de ECA plasmática se determinó por fluorimetría. La actividad enzimática de NADPH oxidasa (unidades relativas de luz por segundo, por mg de proteína; URL/seg/mg/ prot) y la producción de O2 (URL) se determinaron por quimioluminiscencia con lucigenina en aorta y miocardio.