Contrast-enhanced ultrasonography in evaluation of severity of renal artery stenosis / 中华全科医师杂志

Objective:To investigate the diagnostic value of contrast-enhanced ultrasonography (CEUS) in evaluating the severity of renal artery stenosis.Methods:Eighty six patients with suspected renal artery stenosis admitted to Shanxi Provincial People′s Hospital from January 2018 to July 2021 were enrolled in the study. All patients underwent digital subtraction angiography (DSA), Doppler ultrasonography (DUS) and CEUS examinations. With DSA results as gold standard the sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) of CEUS and DUS in the diagnosis of renal artery stenosis were analyzed. The consistency between CEUS and DSA in the diagnosis of renal artery stenosis was evaluated. The accuracy of DUS and CEUS in diagnosis of renal artery stenosis was assessed by the area under receiver operating characteristic (ROC) curve (AUC) and compared between groups.Results:Among 153 renal arteries from 86 patients examined by DSA, 101 showed the stenosis ≥30% (49 left and 52 right renal arteries), and there were 31, 45, 20 and 5 renal arteries with grade I, II, III and IV stenosis. The sensitivity and specificity of DUS in the diagnosis of renal artery stenosis were 74.3% (75/101) and 76.9% (40/52),respectively. DUS showed 21, 42, 20 and 4 renal arteries with grade Ⅰ, Ⅱ, Ⅲ and Ⅳ stenosis, respectively. The sensitivity and specificity of CEUS in the diagnosis of renal artery stenosis were 88.1% (89/101) and 86.5%(45/52), respectively. CEUS showed 26, 43, 23 and 4 renal arteries with grade Ⅰ, Ⅱ, Ⅲ and Ⅳ stenosis, respectively. There was a significant difference between DUS and DSA in grading of renal artery stenosis (χ2=4.447, P=0.03),and there was a moderate consistency between DUS and DSA ( Kappa=0.480). There was no significant difference between CEUS and DSA in grading renal artery stenosis (χ2=0.842, P=0.36) with a good consistency ( Kappa=0.730). The AUC of CEUS in diagnosis of renal artery stenosis was higher than that of DUS [0.873 (95% CI: 0.808-0.938) vs. 0.756 (95% CI:0.673-0.839); Z=4.361, P<0.01]. Conclusion:The study suggests that contrast-enhanced ultrasonography may be used as a diagnostic method for grading renal artery stenosis.

Progress of magnetic resonance imaging in radiotherapy for cancer / 中华放射医学与防护杂志

In recent years,there has been a considerable research effort concerning the application of magnetic resonance imaging (MRI) for the external radiotherapy.This paper reviewed the progress of MRI in radiotherapy for cancer,such as magnetic resonance simulator,target volumes,dose calculation and efficacy evaluation.Furthermore,the potential applications of 4D-MRI and MRI guided radiotherapy are summarized.

Mutation analysis of seven patients with Waardenburg syndrome / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To perform genetic analysis for 7 patients with Waardenburg syndrome.</p><p><b>METHODS</b>Potential mutation of MITF, PAX3, SOX10 and SNAI2 genes was screened by polymerase chain reaction and direct sequencing. Functions of non-synonymous polymorphisms were predicted with PolyPhen2 software.</p><p><b>RESULTS</b>Seven mutations, including c.649-651delAGA (p.R217del), c.72delG (p.G24fs), c.185T>C (p.M62T), c.118C>T (p.Q40X), c.422T>C (p.L141P), c.640C>T (p.R214X) and c.28G>T(p.G43V), were detected in the patients. Among these, four mutations of the PAX3 gene (c.72delG, c.185T>C, c.118C>T and c.128G>T) and one SOX10 gene mutation (c.422T>C) were not reported previously. Three non-synonymous SNPs (c.185T>C, c.128G>T and c.422T>C) were predicted as harmful.</p><p><b>CONCLUSION</b>Genetic mutations have been detected in all patients with Waardenburg syndrome.</p>

Pluronic F127 as a drug vehicle used in chick embryo chorioallantoic membrane shell-less model

The developing vascular network is grown on the surface of chick embryo chorioallantoic membrane [CAM], so CAM is widely used as an in vivo model to study the angiogenesis. Because the CAM is hindered or wrinkled by the vehicle, the drug effect is difficult to be observed. In the present study, we firstly introduced the pluronic F127 aquogel to deliver drugs for the CAM model. The biocompatibility and advantage of this vehicle was shown by applied ranibizumab-pluronic F127 mix on the CAM. The results were showed that, the growth of blood vessels was not impaired by pluronic F127 gel, and the gel was almost imperceptible on the CAM, at the same time, the degradation of blood capillaries caused by ranibizumab was clearly visible. In conclusion, pluronic F127 was a good vehicle for angiogenesis research

Detection of common deafness-related genes among non-syndromic deafness patients from Shanxi province / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To explore the common causative genes and mutation sites for hereditary non-syndromic deafness in Shanxi.</p><p><b>METHODS</b>Peripheral blood samples were collected from regional schools for children with deafness. The samples were analyzed by matrix-assisted laser desorption ionization of flight mass spectrometry, and the results were verified by DNA sequencing.</p><p><b>RESULTS</b>For all samples, the 20 mutational sites of the 4 common causative genes were tested. As revealed, c.235delC of GJB2 gene has the highest mutational rate (13.67%). c.IVS7-2A>G of SLC26A (PDS) gene has a mutation rate of 17.67%, and c.1555A>G of mitochondrial 12S rRNA has a mutation rate of 2.00%. No mutations have been found with GJB3 gene. Sequencing analysis has suggested that the above results have a consistency rate of 99%.</p><p><b>CONCLUSION</b>Analysis of mutations of the 4 common deafness-related genes can facilitate early diagnosis and treatment for the disease. Matrix-assisted laser desorption ionization time of flight mass spectrometry is a reliable method for such a task.</p>

Assessment of right ventricular volumes and ejection fraction in healthy adults by single-beat real-time three-dimensional echocardiography / 中华超声影像学杂志

Objective To analyze the normal reference values of the right ventricular volumes and ejection fraction of healthy adults measured by single-beat real-time three-dimensional echocardiography (sRT-3DE).Methods The subjects of this study included three hundred and thirty-four healthy adults,and were divided into 2 groups by gender and 6 groups by age.The sRT-3DE was chosen to measure their right ventricular volumes and ejection fraction.The parameters of right ventricular volumes were standardized by body surface area (BSA)and lean body mass (LBM)to calculate the ranges of their normal value.The correlation between these parameters and genders as well as the physical characteristics (height,weight, body mass index,BSA and LBM)was also clarified.Results Three hundred and thirty-four (96%)of the 348 subjects had been tested.Before standardizing,men had larger right ventricular volumes compared with women,and the differences of the right ventricular end diastolic volume (RVEDV),right ventricular end systolic volume (RVESV)and right ventricle stroke volume (RVSV)were statistically significant(P 0.05 ).After standardizing by LBM,the RVEDV,RVESV and RVSV in male group were significantly lower than those in women group (P < 0.001 ).In different age groups,the difference of RVEDV,RVESV,RVSV ,but no RVEF were statistically significant (P < 0.05 ).Positive correlations between the RVEDV,RVESV,RVSV and the BSA,height,weight,body mass index and LBM had been detected,and it showed a negative relation between RVEDV,RVESV,RVSV with age.Noticeably,RVEF had no correlation with them.Conclusions sRT-3DE can provide a preliminary range for normal reference values of right ventricular volumes and RVEF in healthy adults according to gender and age and finds that LBM has certain impact on adult cardiac structure,which offers valuable evidence for the diagnosis of right heart diseases in clinical practices.

Mechanical properties and biological evaluation of buffalo horn material / 生物医学工程学杂志

Mechanical properties and biological evaluation of buffalo horn material were examined in this study. The effects of sampling position of buffalo horn on mechanical properties were investigated with uniaxial tension and micron indentation tests. Meanwhile, the variation of element contents in different parts of buffalo horn was determined with elemental analysis, and the microstructure of the horn was measured with scanning electron microscopy. In addition, biological evaluation of buffalo horn was studied with hemolytic test, erythrocyte morphology, platelet and erythrocyte count, and implantation into mouse. Results showed that the buffalo horn had good mechanical properties and mechanical characteristic values of it gradually increased along with the growth direction of the horn, which may be closely related to its microstructure and element content of C, N, and S in different parts of the buffalo horn. On the other hand, because the buffalo horn does not have toxicity, it therefore does not cause hemolysis of erythrocyte and has a good affinity with it. Buffalo horn has good histocompatibility but meanwhile it may induce the platelet adhesion and aggregation. Even so, it does not continue to rise to induce a large number of platelet to aggregate with resulting blood clotting. Therefore, the buffalo horn material has been proved to possess good blood compatibility according to the preliminary evaluation.

Microsatellite instability of D310 and D16184 located in mitochondrial D-loop region in acute leukemia / 白血病·淋巴瘤

Objective To study the microsatellite instability (MSD) of D310 and D16184 located in mitochondrial D-loop region in acute leukemia (AL). Methods The HV-1 and HV-2 regions in D-loop region of 100 persons with the untreated and treated acute leukemia was amplificated and screened by PCR-SSCP,then the abnormal samples was amplificated and sequenced directly and compared with revised Cambridge reference sequence (rCRS) and mtDB. The mutation rates of D310 and D16184 was measured by SPSS11.5 statistics software, x2-test. Results The total mutation rate of D310 was found in 49.0 % (49/100) of our patients. Its mutation rates in untreated group and treated group were 32.5 % (13/40) and 60.0 % (36/60)respectively. The mutation rate of treated group is higher than that in untreated group (P ＜ 0.05). The total mutation rate of D16184 was found in 32.0 % (32/100) of our patients. Its mutation rates in untreated group and treated group were 20.0 % (8/40) and 40.0 % (24/60) respectively. The mutation rate of treated group is higher than that in untreated group (P ＜ 0.05). Conclusion There was a high mutation rate with various types of mutations of microsatellite D310 and D16184 located in mitochondrial D-loop region in AL, which led to a doughty MSI. Chemotherapy could cause a more doughty MSI.

Comparison of delayed enhanced cine MRI, single photon emission computed tomography and echocardiography for the detection of viable myocardium / 中华放射学杂志

of DE-MRI and the specificity and accuracy of LDDSE were significantly lower than that of DE-MRI (P < 0. 05). Conclusion DE-MRI with high specificity and accuracy, potentially, is a valuable imaging method for the detection of viable myocardium.

Evaluation of regional wall motion during myocardial ischemia and reperfusion by quantitive tissue velocity imaging / 中华超声影像学杂志

Objective To discuss the feasibility of quantitive tissue velocity imaging(QTVI) to quantify regional myocardial dysfunction induced by acute ischemia and reperfusion.The results derived from this study provide experimental foundation for QTVI in estimating the segment of myocardial ischemia and evaluating viable myocaridium after reperfusion.Methods Note the peak velocity of the systolic wave(Vs) and the peak velocity of the early diastolic wave(Ve) of regional wall segments of seven dogs with left anterior descending coronary artery(LAD) ligated under the different examination time points(i.e.,pre ligation,basic state;post ligation 5 min,15 min,30 min,60 min,120 min and reperfusion) by using QTVI technique.Postoperation, the myocardial specimen was stained by 1% triphenylatetrazolium chlorde(TTC) solution and tissue structure was analyzed by transmissible electrical microscope to identify whether myocardium was necrosis or not.Results Vs,Ve of every segmental myocardium of the dogs at ligation 5 min,10 min falled evidently in comparison with the basic state of the dogs (P

Preparation and in vitro antioxidative activity of acetylated fucoidan extracted from Laminaria japonica / 中国海洋药物

Objective To explore the optimum method for preparation of acetylated fucoidan extracted from Laminaria japonica,and test the antioxidative activity of acetylated fucoidans prepared under different conditions in vitro.Methods Using acetic anhydride as acetylated reagent,N-Bromosuccinimide(NBS) as catalyst,the effects of reaction time,temperature and volume of acetylated reagent were tested by the orthogonal design method.The antioxidative activity of the prepared acetylated fucoidans in vitro was determined,including scavenging activity against superoxide,hydroxyl and DPPH radical and reducing power.Results Volume of acetylated reagent and reaction temperature were the significant factors(P

Effects of Degraded and Natural Polysaccharides from Porphyra Haitanensis on Proliferation of Lymphocytes / 中药新药与临床药理

Objective To analyze the property of natural polysaccharides(P) from Porphyra haitanensis T. J. Chang et B. F. Zheng and its degraded polysaccharides(P3),and to investigate their effects on proliferation of lymphocytes. Methods P3 and P were identified by IR spectra. Their effects on immunological function were studied by cell culture technique. Results P3 and P displayed significant effects on promoting the proliferation of T-lymphocytes,and had inhibitory effects on the proliferation of B-lymphocytes in a dose-dependent manner. The effects of P3 were more powerful than P. Conclusion The degraded and natural polysaccharides from Porphyra haitanensis(P3 and P) can regulate the proliferation of lymphocytes,which may be related to their molecular weights.