ABSTRACT
ABSTRACT This work presents the methodology, development and testing of an autonomous system, based on Artificial Neural Networks (ANN), for the reduction of technical losses in reticulated underground systems through the optimal control of the capacitor banks (CBs) present in the grid. The proposed methodology includes Smart Grid features, including practical solutions for current transformers positioning in underground networks, collecting field measurements for the Distribution Operation Centre (DOC) and real-time control of field equipment (capacitors banks). The steps of the proposed methodology and the main aspects of the development of the system are also described, as well as the tests performed to prove the results and validate the system.
Subject(s)
Neural Networks, Computer , Electric Wiring , Energy Consumption , Sustainable DevelopmentABSTRACT
Two Atlantic Rain Forest fragments in Minas Gerais state were studied to access their Agaricales fungal richness. A total of 187 specimens were collected and 109 species, 39 genera, and eight families were identified. Thirty-three species were cited for the first time in Brazil.
Subject(s)
Agaricales/genetics , Agaricales/isolation & purification , Biodiversity , Basidiomycota/genetics , Basidiomycota/isolation & purification , Environment , Tropical Ecosystem , Methods , Methods , VirulenceABSTRACT
The fungus Lentinus strigosus (Pegler 1983) (Polyporaceae, basidiomycete) was selected in a screen for inhibitory activity on Trypanosoma cruzi trypanothione reductase (TR). The crude extract of L. strigosus was able to completely inhibit TR at 20 µg/ml. Two triquinane sesquiterpenoids (dihydrohypnophilin and hypnophilin), in addition to two panepoxydol derivatives (neopanepoxydol and panepoxydone), were isolated using a bioassay-guided fractionation protocol. Hypnophilin and panepoxydone displayed IC50 values of 0.8 and 38.9 µM in the TR assay, respectively, while the other two compounds were inactive. The activity of hypnophilin was confirmed in a secondary assay with the intracellular amastigote forms of T. cruzi, in which it presented an IC50 value of 2.5 µ M. Quantitative flow cytometry experiments demonstrated that hypnophilin at 4 µM also reduced the proliferation of human peripheral blood monocluear cells (PBMC) stimulated with phytohemaglutinin, without any apparent interference on the viability of lymphocytes and monocytes. As the host immune response plays a pivotal role in the adverse events triggered by antigen release during treatment with trypanocidal drugs, the ability of hypnophilin to kill the intracellular forms of T. cruzi while modulating human PBMC proliferation suggests that this terpenoid may be a promising prototype for the development of new chemotherapeutical agents for Chagas disease.
Subject(s)
Animals , Cattle , Humans , Mice , Cell Proliferation/drug effects , Enzyme Inhibitors/pharmacology , Lentinula/chemistry , NADH, NADPH Oxidoreductases/antagonists & inhibitors , Trypanocidal Agents/pharmacology , Trypanosoma cruzi/drug effects , Enzyme Inhibitors/isolation & purification , Flow Cytometry , Lymphocytes/drug effects , Monocytes/drug effects , Trypanocidal Agents/isolation & purification , Trypanosoma cruzi/enzymologyABSTRACT
A total of 103 isolates of basidiomycetes, representing 84 species from different Brazilian ecosystems, were evaluated for their antifungal and antibacterial activity in a panel of pathogenic and non-pathogenic microorganisms. Tissue plugs of the fruiting bodies were cultivated in liquid media and the whole culture extracted with ethyl acetate. Crude extracts from Agaricus cf. nigrecentulus, Agrocybe perfecta, Climacodon pulcherrimus, Gloeoporus thelephoroides, Hexagonia hydnoides, Irpex lacteus, Leucoagaricus cf. cinereus, Marasmius cf. bellus, Marasmius sp., Nothopanus hygrophanus, Oudemansiella canarii, Pycnoporus sanguineus, Phellinus sp., and Tyromyces duracinus presented significant activity against one or more of the target microorganisms. Eight isolates were active only against bacteria while three inhibited exclusively the growth of fungi. Two extracts presented wide antimicrobial spectrum and were active against both fungi and bacteria. Differences in the bioactivity of extracts obtained from isolates from the same species were observed.