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Objective:To investigate the implementation effect of micro-video assisted physiological theory teaching based on functional experiments.Methods:There were 140 clinical undergraduates (control group) from Class 1 and Class 2, and 123 clinical undergraduates (experimental group) from Class3 and Class 4 of Batch 2017 in our university who were involved in this study. The control group adopted traditional teaching method, the experimental group adopted micro-videos to assist traditional teaching in the teaching of selected chapters, and these micro-videos were collected from the recording and editing of functional experiments. After the course, questionnaire survey in terms of course design, implementation and effect, as well as final exanimation performance analysis were conducted to evaluate the teaching effect. SPSS 17.0 was used for t test. Results:The final examination scores of the experimental group were significantly higher than those of the control group [(81.02±9.64) vs. (73.41±11.39)], with significant differences ( t=-5.805, P<0.001). Among them, the scores of Chapter 2 of the two groups were [(8.07±0.94) vs. (6.14±1.05), t=-15.616, P<0.001)], the scores of Chapter 4 were [(16.16±1.79) vs. (10.90±2.23), t=-20.903, P<0.001)], and the scores of Chapter 6 were [(6.04±0.53) vs. (5.82±0.78), t=-2.638, P=0.009)], all with significant differences. 100% of questionnaires were recovered, and more than 90% of students were interested in this teaching method which could strengthen their understanding of the key and difficulties in physiology and was also helpful to cultivate their ability of induction and summarization. Conclusion:Micro-videos based on functional experiments assisted teaching can improve the teaching effect of physiology, and it's worth popularizing.
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Objective To investigate the effectiveness of Microlecture plus PowerPoint (PPT) in physiology experiment teaching.Methods The students of clinical medicine in Grade 2015 of Hubei University of Medicine were selected as the subjects.Among them,61 students in Class 1 were studied as the control group,while 59 students in Class 2 as the experimental group.The control group adopted the traditional teaching method and required students to complete the experimental report with handwriting,while the experimental group adopted teaching method of Microlecture plus PPT.In the experimental group,students watched the microlecture for preview and could also repeat watching at any time to correct the mistakes during the operation.After class,students of the experimental group were required to complete the experiment report in the form of PPT and send it to the teacher through QQ within the prescribed time.The learning outcomes were evaluated by using descriptive analysis and t test based on the class operation,final examination and questionnaire.Results Both the average score of experiment operation and the average scoreof final theory exam in the experimental group were higher than those of the control group [(95.074 ± 3.233) vs.(81.706 ± 2.901) and (92.204 ± 3.074) vs.(77.913 ± 2.611)],and the differences were of statistical significance(P＜0.05).The recovery rate of questionnaire survey was 100％ in the experimental group.Over 80％ students thought Microlecture plus PPT could help enhance autonomous learning ability,deepen the understanding of theory and increase group cooperation ability,et al.Conclusion Microlecture plus PPT teaching can improve the teaching effect of physiology experiment teaching,which is worthy of promotion.However,it is still necessary to combine other teaching methods to achieve better teaching results.
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It has been a major topic for the reform on higher medical education and teaching to cultivate medical students' professional information literacy so asto make them equipped with the information competence required for disease control or medical research.In this thesis,the concept of professional information literacy for medical students was introduced,and by analyzing the relationship of information literacy education and medical education modes,countermeasures and methods of cultivating medical students' professional information literacy by combining the reform on medical education and teaching were put forward.
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@#Objective To investigate the effect of pyrroloquinoline quinone(PQQ) on the aging of rat hippocampal neurons induced by D-galactose(D-gal).Methods Hippocampal neurons were cultured in vitro.The aging of the hippocampal neurons was induced by high dose D-gal,PQQ protection were used 30 min before D-gal.The metamorphosis of hippocampal neurons was observed under the microscope.The contents of free radical was measured.The incidence of apoptosis of hippocampus cells was tested with the flow cytometry.The expression of Bax was detected with immunohistochemical staining.Results After the cells cultured in vitro exposed to D-gal,the content of free radical and the expression of Bax of the hippocampal neurons increased.After pretreatment of the cultured neurons with PQQ,the contents of free radical and the expression of Bax decreased,the survival of hippocampal neurons increased.Conclusion PQQ may slow the aging progress of hippocamal neurons induced by D-gal.
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Objective To investigate the effects of pyrroloquinoline quinone(PQQ) on the hippocampal neurons of aged rats induced by D-galactose(D-gal).Methods D-gal was used to induce the model of aging rat,PQQ was administered into rat lateral intracerebroventricle.After 50 days the metamorphosis of hippocampal neurons was observed by H-E and Nissl's staining.The apoptosis rate of hippocampus was tested by flow cytometry.The contents of free radical and C-FOS protern were measured.Results Compared with the control group,the size of the neurosoma was slightly changed,the optical density of Nissl's was decreased,the content of free radical and the apoptosis rate increased markedly in D-gal group.After PQQ injection with D-gal,the size of neurosoma and the optical density of Nissl's were markedly increased,the content of free radical and the apoptosis rate of hippocampus did not change.PQQ improved the expression of C-FOS protern.Conclusion PQQ can slow down the aging progress of hippocamal neurons induced by D-gal.
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@# ObjectiveTo investigate the protective effect of pyrroloquinoline quinone (PQQ) on damage of hippocampal neurons induced by NMDA.MethodsHippocampal neurons were cultured in vitro and NMDA was used to induce neurotoxicity 8 d after, while PQQ were used or not before. The metamorphosis of hippocampal neurons was observed under the microscope. Intracellular calcium levels was measured by confocal laser microscopy. ResultsThe intracellular Ca2+ level increased rapidly after exposure to 0.1 mmol/L NMDA and resulted in neuronal necrosis and apoptosis. Otherwise, pretreatment of the cultured neurons with PQQ reduced the increase of the intracellular Ca2+ level and the neuronal necrosis or apoptosis induced by NMDA.ConclusionPQQ can protect hippocampal neurons from damage induced by NMDA.