Clinicopathological Study on Uterine Fibroid in a Medical College Hospital

Uterine fibroid are the most common benign pelvic tumor in females. Its occurrence in reproductive age group makes it important. Methods: The present hospital based cross-sectional study was done to find the clinicopathological features of fibroid of uterus at a tertiary care center. Histopathological examination was done to observe the type and morphology of these lesions. Results: Uterine fibroids are commonly seen in females of <40 years age and present with menorrhagia (55.2%) and abdominal pain (27.1%). The most common location was intramural (63.5%) and the most common degeneration was hyaline change (12.5%). Conclusion: Routine histopathological examination of hysterectomy specimen is needed to rule out tumor or infective pathology.

Interobserver reproducibility of Gleason grading of prostatic adenocarcinoma among general pathologists.

Context: Gleason grade is the most widely used grading system for prostatic carcinoma and is recommended by World Health Organization. It is essential that there should be good interobserver reproducibility of this grading system as it has important implications in patient management. Aim: To assess interobserver reproducibility of Gleason grading of prostatic adenocarcinoma. Design: A total of 20 cases of prostatic adenocarcinoma were scored using Gleason grade by 21 general pathologists. The scores were then compared using κ-coefficient and consensus score. Results: For Gleason score groups (2-4, 5-6, 7 and 8-10) overall agreement with consensus score was 68%. Exact agreement for Gleason scores with consensus score was 43.3% and 92.3% within ±1 of the consensus score. κ coefficient for primary grade ranged from -0.32 to 0.92 with 60% of the readings in fair to moderate agreement range; and for secondary grade κ ranged from -0.30 to 0.62 with 78% of the readings in slight to fair agreement range. Kappa for Gleason scores ranged from -0.13 to 0.55 with 80% of the readings in slight to fair agreement range; and for Gleason score groups κ ranged from -0.11 to 0.82 with 68.5% of the readings in fair to moderate agreement range. Conclusions: In our study interobserver reproducibility of Gleason scores among general pathologists was at lower level and it highlights the need to improve the observer reproducibility by continuous educational sessions and taking second opinion in cases where grade could significantly influence management.

Improvement of water quality through biological denitrification.

The present study was undertaken to observe the effect of biological denitrification process on water quality parameters. The results obtained in the present investigations show that the value of pH and alkalinity was increased due to generation of alkalinity during biological denitrification process. The obtained value of the DO in the treated water was found lower than the supplied water, which indicates, DO was consumed by the bacterium. The COD of the treated water was nil, which shows that the organics have been consumed by the bacterium during the denitrification process. The biological reduction of nitrate-nitrogen (from 50.79 mg/L to 0.57 mg/L) was found to be lower than the tolerance limit prescribed by WHO without changing the water quality.

The effect of carbon as an energy source for biological denitrification through Pseudomonas stutzeri.

The biological denitrification processes possess many advantages in comparison to other denitrification processes. The present study was undertaken to observe the effect of quantity of carbon on biological denitrification with Pseudomonas stutzeri at different C/N ratios. The results obtained in the present investigations show that the pH and alkalinity of the effluent increased with increase in C/N ratio due to alkalinity generated during denitrification. The COD of the influent was increased with increase in C/N ratio. The effluent COD was nil under pseudo-steady state condition up to the C/N ratio of 2.5. Above this C/N ratio, the organics started entering in the treated water. The maximum feasible economic reduction of NO(3-)N from 51.6 mg/l to 1.2 mg/l occurred at C/N ratio of 2.5 and the obtained concentration of NO(3-)N was found to be lower than the tolerance limit (10 mg/l) prescribed by the WHO. The present work provides a feasible approach for a more efficient NO(3-)N removal process.