ABSTRACT
Aims: The paper aimed to clarify the effect of cucumber target leaf spot (TLS) under the Jingdusha (JDS) treatment. Study Design: We applied the method of artificial inoculation in the pot, and analyzed the changes in growth indexes and physiological characteristics. Place and Duration of Study: In 2018, these experiments were conducted in College of Bioscience and Biotechnology of Shenyang Agricultural University (Lab 240). Methodology: The seedlings in the two-leaf period were induced by the best application scheme of JDS, then inoculated Corynespora cassiicola for 24 h. Cucumber seedlings of each treatment group were randomly selected for photographing and growth index determination after inoculation for 5 d. The leaves of cucumber seedlings in each treatment group were randomly collected at 1 d, 3 d, 5 d, 7 d, and 9 d after inoculation for the determination of physiological and biochemical indicators. Results: When C. cassiicola infects cucumber, JDS can effectively improve the growth and photosynthetic pigment content of cucumber, reduce the degradation of chlorophyll (Chl) under the stress of C. cassiicola, strengthen the variety of metabolic responses in the plant, repair the enzyme protection system of cucumber leaves, reduce the accumulation of reactive oxygen species, shorten the process of membrane lipid peroxidation in blades. Conclusion: Taken together, these results suggest that JDS can improve the resistance of cucumber seedlings to C. cassiicola by regulating growth indexes and physiological characteristics. This work will provide a theoretical basis for further elucidating the molecular mechanism of JDS in cucumber defense against C. cassiicola.
ABSTRACT
Aim: The aim of this study was to test the effects of Korean floor-heating system on heat loss using adult pig models, and to create a novel formula for estimating time since death during the early stages of decomposition. Methodology: Three electric mattress pads were placed on the ground to maintain a constant temperature of the substrate like the ondol heating system. Four temperature measuring probes were placed in each pig: inside the rectum, on the body surface, between the body and the surface of mattress pad and on the mattress pad. The probes were connected to a temperature data logger system. Temperature was recorded every minute and statistical analysis was performed using the SAS (version 9.3) program. Results: Spearman's Rank Correlation results demonstrated the rectal temperature, and the temperature between the body and the surface of pad were strongly correlated with postmortem cooling of the body, rather than ambient temperature. The rate of cooling of the body is represented by a cube function of time rather than an exponential or bi-exponential function. Interpretation: This research indicates that postmortem cooling of the body is more influenced by ground surface temperature than by ambient (environment) temperature, and the rectal temperature fluctuated with the ambient temperature. Additionally, the study showed that pigs can be good animal models that can substitute human cadaver to study the process of decomposition