ABSTRACT
The aim of this article is to characterize the biological aspects of oral strains of C. albicans in children with Down's syndrome. These yeasts were analyzed as to their macromorphological and enzymatic aspects and were tested as to their in vitro susceptibility to antifungal drugs using broth microdilution to determine the minimum inhibitory concentration (MIC). The morphotyping revealed that all oral C. albicans isolates from children with Down's syndrome promoted the formation of fringes regardless of size, while the control group presented smaller fringes. All oral C. albicans strains produced proteinase, but those with phospholipolytic activity showed greater enzyme capacity in the test group. In vitro susceptibility showed that all oral C. albicans isolates were sensitive to the drugs used.
O objetivo deste artigo foi caracterizar os aspectos biológicos de cepas de C. albicans orais em crianças com síndrome de Down. Estas leveduras foram analisadas quanto aos seus aspectos macromorfológicos e enzimáticos e foram testadas quanto a sua suscetibilidade in vitro a drogas antifúngicas, usando a microdiluição em caldo para a determinação da concentração inibitória mínima (CIM). A morfotipagem revelou que todos os isolados de C. albicans orais de crianças com síndrome de Down induziram à formação de franjas independente do tamanho, enquanto o grupo controle teve franjas menores. Todas as cepas de C. albicans orais produziram proteinase, mas aquelas com atividade fosfolipidolítica mostraram maior capacidade enzimática no grupo teste. A suscetibilidade in vitro mostrou que todos os isolados de C. albicans orais foram sensíveis a drogas empregadas.
Subject(s)
Humans , Child , Antifungal Agents/pharmacology , Candida albicans/genetics , Candidiasis, Oral/microbiology , Down Syndrome/microbiology , Phenotype , Case-Control Studies , Candida albicans/drug effects , Candida albicans/enzymology , Microbial Sensitivity Tests , Peptide Hydrolases/biosynthesis , Phospholipases/biosynthesisABSTRACT
A imunização de hospedeiros vertebrados com componentes derivados de vetores pode se constituir numa estratégia alternativa para o controle de doenças transmitidas por insetos. No presente estudo avaliamos o efeito de anticorpos antiflebótomos sobre alguns parâmetros biológicos de fêmeas de Lutzomyia longipalpis, vetor de leishmaniose visceral. Coelhos foram imunizados com extratos de tubos digestivos de fêmeas alimentadas com açúcar (GS), fêmeas alimentadas com sangue (GB), carcaças de fêmeas alimentadas com açúcar (CS) ou carcaças de fêmeas alimentadas com sangue (CB), e coelho imunizado por repetidas picadas de fêmeas de flebótomos (BITE). Os soros imunes de coelhos apresentaram títulos aumentados quando comparados com os soros pré-imunes, e bandas específicas foram detectadas por meio de Western Blot. A análise dos parâmetros biológicos revelou um decréscimo na fecundidade no grupo de fêmeas alimentadas em coelho imunizado com GB e BITE. A longevidade e a mortalidade foram estudadas em fêmeas com postura (paridas) e fêmeas sem postura (nulíparas). Fêmeas nulíparas que se alimentaram em coelho imunizado por repetidas picadas morreram em maior percentual. A análise da mortalidade, após a postura dos ovos, revelou um pico no quinto dia em todos os grupos, mas em fêmeas que se alimentaram em coelho submetido a repetidas picadas, foi antecipada para o terceiro dia.
Subject(s)
Rabbits , Animals , Female , Antibodies/immunology , Immune Sera/pharmacology , Insect Vectors/immunology , Psychodidae/immunology , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Fertility , LongevityABSTRACT
The influence of altitude and latitude on some structure sizes of Lutzomyia intermedia was noted; several structures of insects collected in higher localities were greater, according to Bergmann's rule. This influence was more remarkable in two localities of the State of Espírito Santo, probably due to greater differences in altitude. Comparing insects from different latitudes, more differences were noted in comparisons of insects from low altitude localities than in those of material from higher altitudes. The small number of differences between insects collected in July and in December does not indicate a defined influence of season and temperature on the size of adults. The possible epidemiological implications of these variations are discussed.
Subject(s)
Animals , Male , Female , Altitude , Psychodidae/anatomy & histology , SeasonsABSTRACT
Case records of 68 newborns who required assisted ventilation over a 24 month period were reviewed. Fortyfour (64.7%) received intermittent mandatory ventilation, 10 (14.7%) received nasal CPAP and the remaining 14 (20.58%) received a combination of the above. Some of the indications for ventilation were infections (21), hyaline membrane disease (16), problems related to asphyxia (11), apnea of prematurity (10) and persistent pulmonary hypertension of newborn (5). The overall survival rate was 41.17%. In the CPAP group 90% (9/10) survived, while in the remaining survival was 32.7% (19/58). The best outcome was observed in persistent pulmonary hypertension of newborn (80%) followed by apnea of prematurity (70%) and hyaline membrane disease (43.75). Outcome was poor in conditions related to birth asphyxia (27.2%) and infections (19.05%). Survival rates were higher (44.4%) in babies weighing > 1500g at birth as compared to 40.9% in babies < 1500g. Babies less than 32 weeks gestation had a survival rate of 32% as compared to 46.5% in those over 32 weeks. This difference was not statistically significant. Complications were seen in 12/68 patients (17.6%). Pneumothorax was the commonest followed by sepsis, intraventricular hemorrhage and blocked endotracheal tubes. Babies with hyaline membrane disease had the highest incidence of complications. Analysis of the data with regard to the indications, outcome and complications is presented.