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1.
Article in Chinese | WPRIM | ID: wpr-1031417

ABSTRACT

ObjectiveThis study aims to investigate the effectiveness and potential mechanism of Erxian Decoction (二仙汤) for late-onset depression (LOD). MethodsForty Wistar male rats of 7-8 weeks were divided into 20 each of normal group and youth depression group. Sixty Wistar male rats of 20 months were divided into 20 each of elder group, LOD group and Erxian Decoction group. The rats in youth depression group were modelled with chronic unpredictable mild stress (CUMS) for 6 weeks to build a depression model, and the elder rats in LOD group and Erxian Decoction group were modelled with CUMS for 6 weeks to build a LOD model, with no interventions in the normal group and the elder group. Gastric administration was carried out at the same time of modelling, rats in Erxian Decoction group were given 8 g/(kg·d) of Erxian Decoction by gavage, and rats in the normal group, youth depression group, elder group, and LOD group were given 4 ml/(kg·d) of pure water by gavage, for 6 weeks in each group. Sugar water preference experiment and forced swimming experiment were used to evaluate the depression-like behaviour of rats, absent field experiment was used to evaluate the spontaneous activity ability of rats, and T-maze experiment was used to evaluate the cognitive function of rats; Western blot assay was used to detect neuronal nuclei (NeuN), nestin, doublecortin (DCX) in hippocampal tissue, and zonula occludens-l (ZO-1), folate receptor α (FRα), reduced folate carrier (RFC), and protoncoupledfolate transporter (PCFT) protein expression in choroid plexus; immunofluorescence was used to detect the number of double-positive cells for Ki-67/nestin, the number of double-positive cells for 5-bromodeoxyuracil nucleoside (BrdU)/DCX in hippocampal dentate gyrus, and the protein expression of ZO-1, FRα, RFC, and PCFT in choroid plexus tissues; ELISA technique was used to detect plasma, cerebrospinal fluid, 5-methyltetrahydrofolate (5-MTHF) in hippocampal tissues; Pearson correlation analysis was used to correlate the 5-MTHF content in cerebrospinal fluid and hippocampal tissues and the expression of nestin, DCX, and NeuN proteins in hippocampal tissues of rats in youth depression group and LOD group. ResultsCompared with normal group, rats in youth depression group and LOD group showed reduced sugar water preference, reduced total distance travelled in the absent field, reduced number of times through the grid, prolonged forced swimming immobilisation, reduced hippocampal NeuN, nestin and DCX protein expression, reduced number of Ki-67/nestin double-positive cells in hippocampal dentate gyrus, reduced number of BrdU/DCX double-positive cells in hippocampal dentate gyrus, and reduced expression of ZO-1 and FRα proteins and fluorescence intensity (P<0.05 or P<0.01); the correct rate of T-maze on days 3 and 4 in the rats of youth depression group and on days 2 to 4 in the rats of LOD group significantly decreased, and the content of 5-MTHF in the cerebrospinal fluid and hippocampal tissues of the rats of LOD group significantly decreased as well (P<0.05 or P<0.01). Compared with youth depression group, rats in LOD group showed a decrease in total distance travelled in absenteeism, number of times through the grid, a significant decrease in the correct rate of the T-maze on day 1-4, a decrease in NeuN, nestin, DCX protein expression of hippocampal tissue and the number of Ki-67/nestin double-positive cells, BrdU/DCX double-positive cells of hippocampal dentate gyrus (P<0.05 or P<0.01). Compared with LOD group, rats in Erxian Decoction group had elevated sugar-water preference and total distance travelled in absenteeism and number of times through the grid, shorter forced swimming immobility time, significantly higher correct rate of the T-maze on day 3-4, elevated expression of NeuN, nestin, and DCX proteins in hippocampal tissues, increased number of Ki-67/nestin double-positive cells and BrdU/DCX double-positive cells in hippocampal dentate gyrus, and increased 5-MTHF content in cerebrospinal fluid and hippocampal, and ZO-1, FRα, RFC, PCFT protein expression and fluorescence intensity increased in choroid plexus (P<0.05 or P<0.01). Correlation analysis showed that there was a positive correlation between 5-MTHF content in cerebrospinal fluid (r = 0.466), hippocampal tissue (r = 0.522) and nestin expression in hippocampal tissue (P<0.05). ConclusionErxian Decoction could improve depressive-like behaviour and cognitive impairment in LOD model rats, and its mechanism may promote hippocampal neurogenesis by alleviating structural damage to the choroid plexus of the brain tissue, and improving impaired choroid plexus folate transport.

2.
Chinese Journal of Anesthesiology ; (12): 1062-1066, 2023.
Article in Chinese | WPRIM | ID: wpr-1028430

ABSTRACT

Objective:To evaluate the effect of ultrasound-guided single fascia iliaca compartmentblock (FICB) combined with esketamine on postoperative delirium (POD) in elderly patients undergoing hip fracture surgery.Methods:Sixty-two patients of either sex, aged 60-85 yr, with body mass index of 18-30 kg/m 2, of American Society of Anesthesiologists Physical Status classification Ⅱ or Ⅲ, undergoing hip fracture surgery under subarachnoid anesthesia, were divided into 2 groups ( n=31 each) using a random number table method: single FICB group (group FICB) and single FICB combined with esketamine group (group FICB+ E). Ultrasound-guided FICB was performed on the operated side at 30 min before subarachnoid anesthesia. In FICB+ E group, esketamine was intravenously injected as a bolus of 0.3 mg/kg at 5 min before skin incision followed by an infusion of 0.25 mg·kg -1·h -1 until 30 min before the end of procedure. Patient-controlled intravenous analgesia was used for postoperative analgesia, and tramadol 1 mg/kg was given for rescue analgesia. The pressing times of patient-controlled analgesia, the number of rescue analgesia, and consumption of tramadol were recorded within 48 h after operation. The Ramsay sedation score was used to assess the degree of sedation at skin incision, 30 min after the start of surgery, 30 min before the end of surgery, at the end of surgery, and at discharge from the post-anesthesia care unit. Postoperative delirium (POD) occurred within 7 days after surgery was assessed using the Confusion Assessment Method. The serum concentrations of tumor necrosis factor-alpha, interleukin-6, S100β and glial fibrillary acidic protein (GFAP) were detected by enzyme-linked immunosorbent assay on admission to the operating room and at 3 and 7 days after surgery. The occurrence of adverse drug reactions (respiratory depression, nausea and vomiting, dizziness, somnolence, urinary retention) was recorded. Results:Compared with group FICB, the incidence of POD was significantly decreased within 3 days after surgery, the consumption of tramadol, pressing times of patient-controlled analgesia and the number of rescue analgesia were reduced, Ramsay sedation score was increased at each time point, and the serum concentrations of tumor necrosis factor-alpha, interleukin-6, S100β and glial fibrillary acidic protein were decreased after surgery ( P<0.05), and no significant change was found in the total incidence of POD at 7 days after surgery in group FICB+ B ( P>0.05). Conclusions:Ultrasound-guided single FICB combined with esketamine can provide adequate analgesia and sedation in the perioperative period for elderly patients with hip fractures, reducing the risk of early postoperative (within 3 days) POD.

3.
Article in Chinese | WPRIM | ID: wpr-994254

ABSTRACT

Objective:To evaluate the efficacy of esketamine combined with fascia iliaca compartment-subarachnoid block in optimizing anesthesia in elderly patients undergoing hip fracture surgery.Methods:Sixty-two American Society of Anesthesiologists Physical Status classification Ⅱ or Ⅲ elderly patients of either sex, aged 60-85 yr, with body mass index of 18.5-30.0 kg/m 2, were divided into 2 groups ( n=31 each) using a random number table method: fascia iliaca compartment-subarachnoid block group (FS group) and esketamine combined with fascia iliaca compartment-subarachnoid block group (ES group). In FS group, patients underwent ultrasound-guided fascia iliaca compartment block at 30 min before the operation of subarachnoid anesthesia on the surgical side. In ES group, esketamine 0.25 mg/kg was intravenously administered at 5 min before skin incision based on the fascia iliaca compartment-subarachnoid block. Patient-controlled intravenous analgesia was used for postoperative analgesia, and tramadol 1 mg/kg was intravenously given for rescue analgesia when numerical rating scale score > 4. The pressing times of patient-controlled analgesic pump, the number of rescue analgesia and consumption of tramadol were recorded within 48 h after operation. The occurrence of postoperative adverse reactions (respiratory depression, nausea and vomiting, dizziness, drowsiness, pruritus, illusion, nightmares) was recorded. Results:Compared with FS group, the consumption of postoperative tramadol was significantly decreased, and the pressing times of patient-controlled analgesic pump and the number of rescue analgesia were reduced in ES group ( P<0.05). There were no significant differences in the incidence of postoperative adverse reactions between the two groups ( P>0.05). Conclusions:Combination of esketamine with fascia iliaca compartment-subarachnoid block for hip fracture surgery can raise postoperative analgesia and optimize clinical management strategies in elderly patients.

4.
Journal of China Medical University ; (12): 980-983,994, 2017.
Article in Chinese | WPRIM | ID: wpr-704928

ABSTRACT

Objective To investigate the effect of miR-218-1-3p on the proliferation,cycle,and apoptosis of A549 cells in non-small-cell lung cancer.Methods miR-218-1-3p was transfected into non-small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent,and the expression of miR-218-3p was detected by real-time PC R.Invasion and migration were assayed using the Transwell method.The effect of miR-218-1-3p on the proliferation of A549 cells was assayed by the MTS method.Changes in the cell cycle and apoptosis of A549 cells transfected with miR-218-1-3p was detected by flow cytometry.Changes in indicators related to cell proliferation,cycle,and apoptosis were detected by fluorescence quantitative PCR.Results Compared to the control group,the cell proliferation of A549 cells was significantly inhibited (P < 0.05) and the proportion of cells in the S and G2-M phases was significantly decreased when miR-218-1-3p was up-regulated.In addition,compared with the control group,the early apoptotic rate was significantly increased by up-regulating miR-218-1-3p.We further detected indicators related to cell proliferation,cycle,and apoptosis and found that CYCLIN-D1 and BCL-2 were significantly downregulated.Conclusion miR-218-1-3p may inhibit proliferation,induce cell cycle arrest,and promote cell apoptosis of non-small cell lung cancer A549 cells by regulating CYCLIN-D 1 and BCL-2.

5.
Article in Chinese | WPRIM | ID: wpr-458502

ABSTRACT

BACKGROUND: Cerebrovascular disease often causes dysfunction of the brain nerve, and nerve cel apoptosis is the important factor of cerebral nerve dysfunction. The excessive expression of c-fos can block the transduction of intracelular signal so that producing some apoptosis-promoting factors, which involve in nerve cel apoptosis process after ischemia injury of brain. Bcl-2 is an inhibited factor. It might to be the key to treat ischemic cerebrovascular disease by inhibiting or reducing the apoptosis of nerve cels after ischemia injury. OBJECTIVE: To investigate the therapeutic effect and mechanism of the Total Flavone of Hawthorn Leaf on chronic cerebral ischemia rats. METHODS: A total of 72 healthy male Sprague-Dawley rats were randomly divided into sham surgery group, model group, Total Flavone of Hawthorn Leaf group and ginkgo leaf group. Permanent bilateral carotid artery ligation was used to prepare chronic cerebral ischemia model in the model group, Total Flavone of Hawthorn Leaf group and ginkgo leaf group. Total Flavone of Hawthorn Leaf group and ginkgo leaf group respectively received 140 mg/kg Total Flavone of Hawthorn Leaf and 12.3 mg/kg ginkgo leaf intragastricaly for 36 days from 36 days after model induction. Model group and sham surgery group received 3.5 mL/kg physiological saline intragastricaly. RESULTS AND CONCLUSION: Compared with the model group, the expression of c-fos protein significantly deceased in the Total Flavone of Hawthorn Leaf group (P 0.05). These data indicated that the protective effect of Total Flavone of Hawthorn Leaf on chronic cerebral ischemia was associated with its inhibition of neuronal apoptosis. Its mechanism of anti-apoptosis might be associated with up-regulating expression of Bcl-2, down-regulating expression of c-fos and decreasing Ca2+ content in brain.

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