ABSTRACT
Introduction: In newborns with hemolytic disease of fetusand newborn, exchange transfusion is one of the treatments.The main objective of this study was to review and establishthe practice of exchange transfusion with reconstituted bloodin neonates and to observe fall of bilirubin and also rise inhemoglobin and its comparison with related studies.Material and methods: Total 31 neonates with hemolyticdisease of fetus and newborn were included in thisstudy and exchange transfusion was carried out to treathyperbilirubinemia. Exchange transfusion with O Rh negativecells suspended in AB plasma were done for neonates havingRh hemolytic disease of fetus and newborn and O Rh positivecells suspended in AB plasma were used for exchangetransfusion to ABO hemolytic disease of fetus and newborn.The pre and post exchange transfusion blood samples weretested for serum bilirubin and hemoglobin.Result: Out of the 31 cases, 20 were of Rhesus (Rh) hemolyticdisease of fetus and newborn, while ABO and other bloodgroups constituted 08 and 03 hemolytic disease of fetus andnewborn cases respectively. The average post-exchange fallin serum indirect bilirubin was (53.47%) and average rise inhemoglobin level was 3.06 gm/dl in all 31 cases.Conclusion: The reconstituted blood is immunologically muchsafer and better than whole blood for purpose of exchangetransfusion in hemolytic disease of fetus and newborn becauseof its superiority in minimizing transfusion reactions and inachieving all the therapeutic effects of exchange transfusionin better way
ABSTRACT
Background: In Transfusion Medicine, screening for suitable donor for platelet aphaeresis is difficult and time consuming procedure. This might lead to delayed supply of life saving Single Donor Platelet to critically bleeding patient. Aims & Objective: The aim of this study was to analyse the effect of various donor and procedure related parameters on the yield of single donor platelet so that the transfusionist as well as clinician can screen blood donors effectively in less time. Pre-donation platelet count, haemoglobin, haematocrit & weight were included as donor related variables. Processing time and blood volume processed were assessed as procedure related variables. Material and Methods: A total number of 265 platelet aphaeresis procedures were performed on CS 3000 plus with AMS cell separator (Fenwal, USA) using closed & open system aphaeresis kits & studied with respect to donor’s, procedure’s & patient’s related data. The statistical analysis used in this study was Pearson Correlation (‘r’ value). Results: The mean pre-donation platelet count was 286 ± 55 x 103/cu mm & mean platelet yield of all procedures was 3.3 ± 0.68 x 1011. Conclusion: Platelet yield correlated positively with pre-donation platelet count (r = 0.302, P < 0.0001). Donor’s weight, haemoglobin & haematocrit were not correlated with the yield & did not affect the yield of single donor platelets.