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Objective To establish the cat models of acute morphine dependence, and explore the etiopathogenesis by investigating its electrophysiology and pathology changes of its brain tissue to provide pathological and electrophysiological evidences for clinical treatment. Methods Twelve cats were randomly divided into control group (n=3) and morphine dependent group (n=9); cats in the morphine dependent group were induced by injection of morphine hydrochloride in a daily increased manner, and at the same time, cats in the control group were injected physiological saline. After the success of model making, EEG was performed on the cortex, hippocampus and nucleus accumbens in cats of the 2 groups; the changes of every encephalic region and pituitary gland were observed under light microscope and electron microscope. Results Morphine dependent cat models were successfully established. EEG indicated that physiological waves decreased and pathological waves appeared frequently in cats of the morphine dependent group as compared with those in the normal ones.Physiological examinations showed that the neuronal degeneration, organelle reduction, mitochondria swelling, chromatin margination and karyopycnosis or even nuclear necrosis occurred in the cortex of every encephalic region. Conclusion Pathological changes and brain damages will appear under EEG after long-term use of morphine, which is intimately related to neuron ultramicrostructure changes.
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Objective To explore the operative treatment for epilepsy in the diastema of the central region. Methods We analyzed the data of 6 patients with epilepsy in the diastema of the central region,receiving surgical treatment in our hospital from Jun 2007 to Apr 2008.All the patients were performed conventional pre-operative assessments,including MRJ,scalp EEG,MEG,high-density scalp EEG,intracranial EEG and cortical electrical stimulation,to locate the epileptogenic zone and functional regions.Under the cortical EEG monitoring,surgical resection and pathological examination were performed,respectively.EEG characteristics and treatment outcomes,including seizure and complications,were followed up after surgery. Results Epileptogenic zones were localized in the medically refractory diastema of the central region.Five patients were seizure free(Engel Ⅰ)after surgery and 1 had rare seizures(Engel Ⅱ).Pathological examination demonstrated 3 with focal cortical dysplasia,and 1 with gliosis.1 with lipoma and 1 with cavernous hemangioma.Three were showed generally normal EEG and another 3 were showed a small amount of slow-waves by postoperative EEG.Conclusion Epilepsy in the diastema of the central region can be precisely resected without serious complications.
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Objective To study the dosimetry of different arrangements of 125I seeds in one plane.Methods Nine different in-plane arrangements of 9 125I seeds (2.035 × 107 Bq/seed) were simulated according to distance (cm) along x (horizontal)- and y( longitudinal )-axis using the 3-dimensional treatment planning system (TPS) (3D-TPS): x0.5, y0. 5; x0. 5, y1.0; x0. 5, y1.5; x1.0, y1.0; x1.0, y1.5;x1.5, y1.5; x0. 5, y0. 5 (2)1.0; x0.5, y1.0 (2)0.5; x1.0, y1.0 (2)0.5. The isodose curves of 40,80, 130, 145 and 200 Gy were created and the area, radius and medical cost under the 40, 80, 130, 145and 200 Gy isodose curves were calculated. Results The area, radius and medical cost under the same isodose curves were significantly different with each 125I seed arrangement. The arrangements which had the biggest area under curves of 40, 80, 130, 145 and 200 Gy isodose were x1. 5, y1. 5; x1. 0, y1. 0; x1. 0,y1. 0; x0. 5, y1. 0 and x0. 5, y1. 0, respectively. Conclusion The matched peripheral dose and therapeutic effect were affected significantly by the geometric arrangement of 125I seeds.
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<p><b>OBJECTIVE</b>To investigate the status of the temporomandibular joint (TMJ) in patients with complaints of joint pain for improving clinical therapy.</p><p><b>METHODS</b>Twenty three joints in twenty patients who complained of TMJ pain were examined radiographically and arthroscopically.</p><p><b>RESULTS</b>There were at least one and more to six pathological changes could be found arthroscopically in the 23 temporomandibular joints, which were different in some respects with radiographic findings.</p><p><b>CONCLUSION</b>TMJ related pain may be associated with pathological alterations in the TMJ, and synovitis may be one of the causes of TMJ pain.</p>
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Adult , Humans , Arthroscopy , Pain , Synovitis , Temporomandibular Joint , Temporomandibular Joint DisordersABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of IL-24 expression on the growth of glioma cells.</p><p><b>METHODS</b>The IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor.</p><p><b>RESULTS</b>It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.</p><p><b>CONCLUSION</b>IL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.</p>