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OBJECTIVE To investigate the effects and mechanism of PCB118 on cell-matrix and cel-cel adhesion in human hepatocel ular carcinoma cel s. METHODS Human hepatocel ular carcinoma cel s BEL-7402 were treated with PCB118 0.1,1.0 and 10.0 nmol · L-1 for 4 or 6 d,respectively. Then the cell-matrix adhesion assay and cell aggregation experiments were conducted to study the effect of PCB118 on cell-matrix adhesion and cell-cell adhesion in BEL-7402 cells. Quantitative real-time PCR and Western blotting methods were employed to assess the expression of key cytokines CD29,N-cadherin and E-cadherin. RESULTS The results showed that the cell-matrix adhesion ability of human hepato?cellular carcinoma cells BEL-7402 were significantly increased(P<0.05)after treatment with PCB118 0.1,1.0 and 10.0 nmol·L-1 for 6 d,whereas the cell-cell adhesion ability was significantly reduced(P<0.05). Exposure to PCB118(0.1,1.0 and 10.0 nmol·L-1)for 6 d induced significant upregulation of the mRNA expression levels of CD29 and N-cadherin along with the downregulation of E-cadherin(P<0.05). Western blotting analysis revealed that PCB118 exposure significantly increased protein expressions of CD29 and N-cadherin but reduced E-cadherin protein level(P<0.01). CONCLUSION PCB118 exposure affects the expression of CD29,N-cadherin and E-cadherin, which may be involved in PCB118-induced alteration of cell adhesion of hepatocellular carcinoma cell line BEL-7402.
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Objective To assess clinical and urodynamic results of augmentation enterocystoplasty (AE) in patients with ncurogcnic hladdcr dysfunction (NBD).Methods Retrospectively reviewed our database between 2005-2011 to identify 77 patients who underwent AE ( sigmoid 74 patients,ileum 3 patients).Postoperativc complications,renal function,urodynamics parameters and quality of daily life were evaluated.Results The mean follow-up length was 24 months.Compared with pre-operative condition,the mean bladder capacity significantly increased from (160.6 ± 128.3) to (468.5 ± 60.6) ml (P ＜0.001 ) and the maximum detrusor pressure decreased from ( 31.1 ± 26.4) to ( 10.9 ± 4.5 ) cm H2O ( P =0.002 ).Serum creatinine level decreased from (270.3 ± 113.6 ) to ( 174.4 ± 81.3 ) μmol/(l) ( P =0.00 1 ).There were significant decrease on mean number of incontinence episodes and pads used per day ( P ＜0.01 ).Post-operative complications included metabolic acidosis in two patients (2.6％),adhesive intestinal obstruction in four patients (5.2％),deteriorating renal function in one patients ( 1.2％ ) and recurrence of vesicoureteral reflux in three patients (3.9％).Conclusions The results suggest that AE is safe and effective in treating patients with NBD.Concomitant URI is considerèd on patients with long illness history,vesicoureteral reflux at low intravesical pressures and upper urinary tract dilation.Patients with moderate and severe upper urinary tract deteriorations benefit from this procedure and the benefit can maintain a long time.
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@#ObjectiveTo study risk factors of urinary calculus formation in spinal cord injured patients. MethodsThe clinical data of 128 patients with spinal cord injury following urinary calculi were retrospectively reviewed.ResultsAmong the 128 cases, there were 32 cases receiving bladder stoma; 34 cases, regular replacement of indwelling catheter; 12 cases, intermittent catheterization; 19 cases, triggered reflex voiding; 11 cases, voiding by abdominal straining; 20 cases, condom catheters with urine collection devices. 120 cases presented with urinary tract infection, and 11 cases presented serum calcium increase. Video urodynamic suggested detrusor areflexia in 39 cases, detrusor overactivity in 63 cases, detrusor external sphincter dyssynergia in 41 cases, detrusor bladder neck dyssynergia in 11 cases, external urethral sphincter overactivity in 27 cases, and urethral sphincter deficiency in 11 cases. The pathology of several physiological conditions coexisted in some patients.ConclusionBladder management after spinal cord injury have a major impact on urinary stones formation. Low urinary tract infection, detrusor-urethral sphincter dyssynergia and other lower urinary tract dysfunction, long-term indwelling urinary catheter and cystostomy were main risk factors for urinary calculus formation. The abnormal calcium metabolism after spinal cord injury may be a risk factor for calculus formation.
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@#Parkinson disease (PD) is one of the common neurological conditions with chronically progressed dyskinesis. 35%～70% of them are perplexed by micturition disorder, which frequently occurs in advanced stage. Urodynamics shows detrusor's overactivity and reflexic incontinence complete voiding. Treament at present includes mainly anticholinergic agents, infusing agents in bladder and/or Botulinum toxin multipoint injections to detrusor. Its genesis and effect is related to deterioration of dopaminergic neurons, hovever, and the mechanism is needed to be elucidated.
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@#Objective To assess the incidence of urinary tract infection after urodynamic studies for spinal cord injury patients. Methods 86 patients with spinal cord injury were underwent urodynamic studies,all paients were undertaken uronoscopy and urine culture before and after urodynamic studies. Group A applied no special treatment after urodynamic studies, group B applied preventive antibiotic treatment. Results The incidence rate of urinary tract infection after urodynamic studies in group A was 8.81%, 7.32 % in group B. There was no significant difference between two groups. Conclusion Urodynamic studies are safe, well-tolerated procedures, need not use prophylactic antibiotic after urodynamic studies.
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@#Objective To explore the videourodynamic manifestation of neurogenic bladder after operation for lumbar intervertebral disk hernia. Methods A consecutive series of 25 patients with neurogenic bladder dysfunction after operation for lumbar intervertebral disk hernia were analysed retrospectively. They were respectively divided into L4-5 and L5-S1 groups according to the focus, or normal, injured and no-drawn groups according to saddle sensation. Their videourodynamic manifestation were analyzed statistically. Results Videourodynamic manifestation including bladder sensation,bladder compliance, stability, bladder neck's opening extent, bladder capacity and urethral pressures were not significantly different between the group L4-5 and L5-S1 (P>0.05), nor was the saddle sensation (P=0.51). But the bladder sensation and urethral pressures were related with the saddle sensation (P<0.05). Conclusion The videourodynamic changes may not be related with the focus, but the saddle sensation in patients after operation for lumbar intervertebral disk hernia, which need further elucidation.
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@#Objective To investigate the impact of detrusor hyperactivity with impaired contractile function(DHIC)on voiding function in benign prostatic hyperplasia(BPH).Methods68 patients with BPH accepted the test of urodynamics.The urodynamic parameters between DHIC group and no DHIC group were compared statistically.ResultsThe residual urine in DHIC group was larger and the detrusor pressure at Qmax,the bladder volume were smaller than that in no DHIC group(P<0.01).ConclusionDHIC worsens the voiding function of the patients with BPH,and the test of urodynamic is helpful to diagnose and to treat it.
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Objective:This study is designed to analyze the relationship between different modes and the effect of bilingual teaching of Pathology in medical colleges for nationalities and improve the bilingual teaching efficiency of medical college for nationalities.Methods:We conducted a mode-control study to analyze the association between the three Chinese-English Teaching Modes [namely:Chinese(100%)Schoolteaching Mode with English(100%)multimedia courseware(Mode1),Chinese(100%)Schoolteaching Mode with Chinese-English multimedia courseware(Mode2),and Chinese(50%)and English(50%)Schoolteaching Mode with Chinese-English multimedia courseware(Mode 3)] and the levels of medical basic and medical English for pathology of the students(139),who had learned the course of pathology by ways of different bilingual teaching modes,from The Nursing-Speciality For Undergraduate Students(Majors In English),by Logistic Regression Analysis methods.Results:Compared with the individuals learning by Mode 1 or 2,the students who learned by Mode 3 possessed higher levels of medical basic and medical English for pathology(adjusted ORs=7.33 and 9.53,respectively,P