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@#In order to study the involatile chemical components in Moutai-flavored distiller’s grains, the Moutai-flavored distiller’s grains were extracted with 75% ethanol, followed by extraction with petroleum ether, ethyl acetate, and n-butanol. Silica gel, ODS, sephadex LH-20, and preparative HPLC were used to separate and identify the petroleum ether and ethyl acetate layers.ESI-MS and NMR were used to identify the compounds, which were respectively identified as pentadecanoic acid (1), palmitic acid (2), trans-2-decenoic acid (3), n-nonyl octadecanoate (4), ethyl octadecanoate (5), ethyl linoleate (6), luric acid (7), 1, 3-dicaprylyl-2-linoleylglycerin (8), cyclic (phenylalanine-proline) (9), cyclo-(proline-leucine) (10), 3, 6-bis-(2-methylpropyl)-2,5-dione piperazine (11), 4-hydroxyphenethyl alcohol (12), 2,4-dihydroxybenzoic acid (13), stigmasterol (14), 2-furancarboxylic acid (15), valine (16), L-alanine acyl-L-proline (17), dihydroquercetin (18), 5, 7, 3'', 4''-tetrahydroxyflavonoids (19), quercetin (20), and naringenin (21). Compounds 1-21 were isolated from distiller’s grains for the first time.
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Objective@#To explore refractive development of primary school students in Jinshan district of Shanghai during the past five years.@*Methods@#A total of 201 first grade pupils enrolled in 2013 were followed up for 5 years. Annual examination of non-cycloplegic refraction and axial length (AL) was implemented and analyzed.@*Results@#The mean spherical equivalents (SEs) of boys at each grade was (0.22±0.53)(-0.04±0.64)(-0.36±0.92)(-0.74±1.23)(-1.14±1.67)D, respectively; for girls, (0.26±0.88)(-0.03±1.02)(-0.28±1.02)(-0.64±1.32)(-1.13±1.65)D, respectively. The mean ALs of boys at each grade was (22.94±0.60)(23.13±0.68)(23.45±0.69)(23.65±0.81)(24.03±0.93)mm, respectively and was (22.40±0.67)(22.67±0.70)(22.95±0.74)(23.14±0.79)(23.59±0.90)mm for girls at each grade, respectively. There were negative correlations between dioptres and ALs in each grade(r=-0.26, -0.35, -0.41, -0.53, -0.59, P<0.05).@*Conclusion@#The dioptre and AL among primary school students in Jinshan district of Shanghai increased gradually and results in developing into myopia. The dioptre negatively associates with AL, which should be both paid attention to among primary school students.
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Objective:To explore the relationship between friendship quality and loneliness and its influent factors like the measuring tool of friendship quality,the participants groups and cultural background. Methods:The studies on the association between the friendship quality and loneliness were collected through the databases of Chi-na National Knowledge Infrastructure,Wan Fang,Wiley Online library,Web of Science,Google Scholar and Duxiu from 1984 to 2014. Meta-analysis was conducted with the use of the CMA2. 0. Estimated value (r)and their 95%confidence interval (95%CI)was calculated to analyze the association between the friendship quality and loneliness from normal subjects except physiological disease (such as autism,etc. ). Results:Totally 46 literatures were met the requirements of meta-analysis with sample size of 19422,including 24 researches With 11846 participants who were from eastern countries,and 22 studies with 7676 western people. There was significant negative correlation of friendship quality and loneliness (r=-0. 28,95%CI:-0. 23--0. 32). The friendship quality measuring tool was able to adjust the relations of friendship quality and loneliness (Q=11. 95,P0. 05 ). Conclusion:It suggests that friendship quality has an influence on lone-liness. Measuring tools and subjects groups effect the relationship friendship quality and loneliness except cultural background.
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Objective To revise the trait self-control scale for Chinese college students.Methods According to the theory,12 items were selected,then data were collected from a sample of 549 and 133 college students of Harbin.Explorer Factor Analysis,Confirmatory Factor Analysis,reliability test and validity test were used.Results ①Trait self-control scale for college students included eight items,two dimensions were restrained and impulsivity,two dimensions can explain 42.90％ of the total variance.②Confirmatory Factor Analysis showed that the two-dimensional structure was reasonable (x2/df =1.24,CFI =0.97,GFI =0.96,AGFI =0.92,IFI =0.97,TLI =0.96,RMSEA =0.04).③The restrained factor scores were positively correlated with satisfaction of life scores and adaption scores(r=0.23,0.48,P＜0.01),while negatively correlated with negative affect (r=-0.19,P ＜0.01).The impulsivity factor scores were negatively correlated with satisfaction of life scores and adaption scores (r=-0.18,-0.34,P＜0.01),while positively correlated with negative affect(r=0.22,P＜0.01).④The composite reliability coefficients of the scales were good:the coefficient of the subscale restraint was 0.69,and the coefficient of subscale of impulsivity was 0.64,the coefficient of the whole scale was 0.80.The two months test-retest reliability coefficient of 133 subjects was 0.66.Conclusion The revised scale of trait self-control scale has good psychometric quality and can be used in Chinese college students.
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Objective To explore the relationship among just-world belief,optimism and life satisfaction in colleges.Methods According to convenience sampling principle,370 colleges were selected and assessed with the just world scale for college students (JWS),satisfaction with life scale (SWLS) and the life orientation test-revised (LOT-R) ;and data were analyzed using correlation analysis and regression analysis.Results ①The scores of just-world belief,optimism and life satisfaction were (3.22 ± 0.46),(3.50 ± 0.57),and (3.70 ± 1.21),all of which were higher than middle points.②The scores of just-world belief were positive correlated with the scores of optimism and life satisfaction (r =0.44,0.36,P ＜ 0.01),and the scores of optimism also were positive correlated with life satisfaction(r =0.38,P ＜ 0.01).③ The just-world belief and optimism can positively predict life satisfaction directly(β =0.24,0.29,P ＜ 0.01),and the two factors had interaction(β=0.09,P＜ 0.05).Among optimistic students,life satisfaction of low just-world belief ones were much worse than that of high just world belief ones.However,among pessimistic students,the differences of life satisfaction between high and low just-world belief ones became little.Conclusion Just-world belief of colleges has significant predictive effect on life satisfaction,and the two factors had interaction.
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Objective:To investigate the inhibitory effect of recombinant human endostatin(ES)combined with rAd/P53 on transplanted human breast cancer cell MCF-7 in nude mice.Methods:Animal breast cancer model was established by inoculating MCF-7 cells in nude mice.The animals were randomized into control group,ES group,rAd/P53 group,and ES+ rAd/P53 group.The tumor growth was observed in each group and immunohistochemical method was employed to examine the microvessel density(MVD) and the expression of vascular endothelial growth factor(VEGF).Results:Tumor growth was significantly inhibited in all the 3 treatment groups(P
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Background and purpose:Multidrug resistance(MDR)is the main obstacle of chemotherapy in the treatment of cancer,and it has been reported that the muted p53 gene is related to MDR.In this study,we explored whether adenovirus mediated p53 gene(Ad-p53)could reverse MDR of human breast cancer and its impacts on the expressions of P-gp,TOPOⅡ and GST-?.Methods:In this study,adriamycin-resistant human breast carcinoma cells(MCF-7/Adr)and its parental cells(MCF-7)were used to determine the effect of Ad-p53.Cck-8 assay was adopted to evaluate the cytotoxicity of adriamycin.Western blot were performed to observe the expression of P-glycoprotein(P-gp),TopoismeraseⅡ(TOPOⅡ)and GST-?.Results:After transfection with 50 MOI Ad-p53,the 50% inhibitory concentration(IC50)of adriamycin to MCF-7/Adr cells was decreased from(4.54?0.91)?g/ml to(0.26?0.11)?g/ml,and the chemosensitivity increased 18.1 times(P
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Background and purpose:There is a tendency to treat tumors through multiple genes and multiple targeting but there has been no reports about the therapeutic effect of recombinant human endostatin(ES)combined with rAd/p53 on graft model of human breast cancer.Methods:MCF-7 human breast cancer cells were inoculated in nude mice.Then the nude mice with xenograft tumor were randomized into groups of control,ES,rAd/p53,and ES + rAd/p53,they were treated according to the plan.Diversity of the xenograft tumor volume and side effect was observed.Microvessel density(MVD)and expression of vascular endothelial growth factor(VEGF)were detected by immunohistochemistry.Results:After the administration of ES,rAd/p53,ES + rAd/p53,the growth of tumor was inhibited with the inhibitory rate of 1.75%,43.36% and 58.68%,respectively.Inhibition of tumor growth was significant in Group rAd/p53 and ES + rAd/p53(P0.05).Conclusions:recombinant human endostatin combined with rAd/p53 can greatly inhibit growth of xenograft tumor,and reduce the generation of capillary vessels.
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Objective To evaluate the cooperative effect of intraperitoneal adenoviral p53 gene therapy together with thymosin ?1 against peritoneal carcinomatosis. Methods KM mouse model bearing peritoneal carcinomatosis was established using H22 cell line. 48 hours after inoculation, adenoviral-mediated p53 gene was administrated intraperitoneally alone, or combined with thymosin ?1 within 7 days. The abdominal girth, body weight, tumor cell number, apoptosis and cell cycle were tested using methods of cell counting and flow cytometry. Results Adenoviral p53 gene transfer alone arrested H22 cell in G0/G1. In combination therapy group, ascitic fluid was obviously halted, tumor cells in peritoneal cavity decreased in number and ascites formation, dead cell ratio was elevated. Conclusion Adenoviral-mediated p53 gene could block cell proliferation. Intraperitoneal adenoviral p53 gene therapy combined with thymosin ?1 showed synergism for the lethal damage and inhibition for peritoneal carcinomatosis.