Role of peripheral blood lymphocyte subsets in echinococcosis-induced sensitization in mice / 中华微生物学和免疫学杂志

Objective:To establish a BALB/c mouse model of Echinococcus granulosus allergy and investigate the role of lymphocyte subsets in Echinococcus granulosus-induced sensitization. Methods:Echinococcus granulosus was isolated from the liver of sheep naturally infected with Echinococcus granulosus and cultured for 40 d. BALB/c mice were intraperitoneally injected with 50 microcapsules and sensitized by intraperitoneal injection of 0.1 ml/10 g of larval Echinococcus granulosus capsule six months after infection. According to the symptom scores 1 h after sensitization, these mice were divided into two groups: non-sensitized group ( n=6) and sensitized group ( n=6). The mice ( n=6) in control group were injected with sterile saline. Blood sample was collected from the angular vein of each mouse. Flow cytometry was used to detect B cells, NK cells and CD3 +/CD4 +/CD8 + T cells. Cytometric bead array was used to measure IL-4, IL-6 and IL-13. Results:The percentage of B cells was significantly higher in the non-sensitized group than in the control group ( P<0.001), but no significant difference was observed between the sensitized group and the control group. Compared with the non-sensitized group, the percentage of B cells in the sensitized group decreased significantly ( P<0.01). Compared with the control group, the percentages of NK cells in the non-sensitized group and the sensitized group decreased significantly ( P<0.001 and P<0.01). Compared with the non-sensitized group, the percentage of NK cells in the sensitized group increased significantly ( P<0.05). Compared with the control group, the percentage of CD3 + and CD4 + T cells in the non-sensitized group decreased significantly ( P<0.05), while the percentage of CD8 + T cells increased significantly ( P<0.01). Compared with the control group, the percentage of CD3 + and CD4 + T cells in the sensitized group increased significantly ( P<0.05 and P<0.001), while no significant change in the percentage of CD8 + T cells was detected. Compared with the non-sensitized group, the percentage of CD3 + and CD4 + T cells in the sensitized group increased significantly ( P<0.05), while the percentage of CD8 + T cells decreased significantly ( P<0.01). The levels of IL-4, IL-6 and IL-13 were significantly higher in the non-sensitized group than in the control group ( P<0.001). Compared with the control group, the sensitized group showed increased IL-4 ( P>0.05), significantly increased IL-6 ( P<0.01) and decreased IL-13 ( P>0.05). The levels of IL-4, IL-6 and IL-13 in the sensitized group were significantly lower than those in the non-sensitized group ( P<0.001). Conclusions:The BALB/c mouse model of allergy induced by larval Echinococcus granulosus was successfully established. This study proved that the humoral immune response induced by Th2 cells played an important role in Echinococcus granulosus-induced sensitization, which provides an important scientific basis for establishing a prevention and treatment strategy for patients with anaphylactic shock caused by extravasation of Echinococcus granulosus fluid.

CT Rotation Motion Control System / 中国医疗器械杂志

The paper introduces a new design of the CT rotation motion control system. The system is controlled by a variable frequency drive as a controller and a general induction motor as an actuator. In addition, this paper also introduced the model selection of CT rotating control device and the parameters setup for variable frequency drive. Meanwhile, a test method of the rotor position servo control system is also introduced at the end of the paper. The new system has lowcost, high-performance features, fully meets the requirement of CT rotation motion control.

Effect of different shear stress on the expression of IL-8 and ET-1 in vascular endothelial cells / 中国医师杂志

Objective To investigate the effect of different shear stress on the expression of Interleukin-8 (IL-8) and endothelin-1 (ET-1) in vascular endothelial cells.Methods Umbilical vein endothelial cell line (ECV-304) was used as the research cell.The shear stress of 5 dyne/cm2 and 10 dyne/cm2 was regulated by the self-made shearing stress control device.The shear stress in were maintained at 0,5 dyne/cm2 and 10 dyne/cm2 respectively in the control group,the low shear stress group and the high shear stress group.The effects on the levels of IL-8 and ET-1 were analyzed between the three groups at five time points of 30 min,60 min,120 min,300 min,420 min.Pearson correlation analysis was used to analyze the correlation between IL-8 and ET-1.Results The level of IL-8 in the three groups has been on the rise.At the three time points of 30 min,60 min and 120 min,there was no statistically significant difference in IL-8 level between the three groups (P ＞ 0.05).At the two time points of 300 min and 420 min,the difference of the IL-8 level in the three groups was statistically different (P ＜ 0.05),At the two time points of 300 min and 420 min,the IL-8 level in the low shear stress group was higher than that in the control group and the high shear group,the IL-8 level in the high shear group was higher than that in the control group (P ＜ 0.05).The level of ET-1 in the control group and the low shear group showed an upward trend,and the ET-1 level in the high shear group increased continuously in the former 120 min,but began to decrease continuously since then.At the three time points of 30 min,60 min and 120 min,the difference of the ET-1 level in the three groups was statistically different (P ＜ 0.05).The ET-1 level of the high shear group at three time points of 30 min,60 min and 120 min was higher than that of the control group,the level of ET-1 at two time points of 30 min and 60 min was higher than that in low shear stress group (P ＜ 0.05),the ET-1 level of the low shear group at two time points of 60 min and 120 min was higher than that of the control group (P ＜ 0.05).At the two time points of 300 min and 420 min,there was no significant difference in the level of ET1 between the three groups (P ＞ 0.05).Pearson correlation analysis showed that there was a positive correlation between the expression of IL-8 and ET-1 (r =0.384,P =0.031).Conclusions Shear stress can affect the expression of IL-8 and ET-1 in vascular endothelial cells,the shear stress of 5 dyne/em2 can increase the level of IL-8 more efficiently,the shear force of 10 dyne/cm2 can increase the level of ET-1 more obviously,but it may cause damage to the vascular endothelial cells.

The relationship among depression, anxiety, stress and addictive substance use behavior in 5 935 secondary vocational students / 中华预防医学杂志

Objective@#To investigate the relationship among depression, anxiety, stress and addictive substance use behavior in secondary vocational students.@*Methods@#Cluster sampling method and the Adolescent Health-related Behaviors Questionnaire were used to collect demographic characteristics, psychological symptoms, and addictive substance usage among 5 935 students in nine vocational schools in Chongqing, Zhaoqing, Ningbo, and Taiyuan. Multivariate logistic regression analysis was used to analyze the relationship between the addictive substance use behavior and psychological factors.@*Results@#The detection rates of depression, anxiety and stress were 46.5% (n=2 762), 58.7% (n=3 483), and 29.8% (n= 1 770), respectively. The prevalence of addictive substances was 74.8% (n=4 440), traditional drugs was 0.8% (n=50), new drugs was 2.8% (n=166), other addictive drugs was 4.1% (n=241). Multivariate logistic regression analysis showed that compared with the normal psychological states of secondary vocational students, the OR value of mild depression tendency alcohol and tobacco use behavior of secondary vocational students was 1.45; the OR values of mild anxiety, moderate anxiety, severe anxiety and very serious anxiety were 1.46, 1.46, 1.71, and 1.83, respectively; the traditional drugs use behaviors were 5.51, and 2.61, respectively, for the severe anxiety and very serious anxiety. Compared with the normal psychological state of secondary vocational students, the OR values of the severe anxiety and very severe anxiety were 2.56, and 2.66, respectively, for severe anxiety and very serious anxiety. Compared with normal psychological status of secondary vocational students, the OR values of mild, moderate, severe, and very severe anxiety were 2.14, 2.47, 2.39, and 3.45, respectively; all P values <0.05.@*Conclusion@#Anxiety and mild depression were risk factors of tobacco and alcohol use in secondary vocational students; severe and above anxiety were the risk factors of drug use in secondary vocational students; anxiety was the risk factor for other addictive drug use in secondary vocational students.

Neutralization of anti-venom IgY against main activities of Deinagkistrodon acutus venom / 中国药理学通报

Aim To evaluate the potency of anti-D. acutus venom IgY neutralizing the main activities of D. acutus venom.Methods After mixing the different a-mounts of IgY with snake venom and incubating togeth-er,the main activities of snake venom were assayed by biochemical methods.Results The in vitro assays in-dicated that anti-D.acutus venom IgY obviously neu-tralized the activities of PLA2 ,5′-nucleotidase,hyalu-ronidase,metalloprotease and serine proteinase (fi-brinogenase)in D.acutus venom.Mouse experiments showed that the ED50 value of IgY for mouse was 1 131.09 μg.Conclusion Anti-D.acutus venom IgY antibodies have good effects in neutralizing D.acutus venom without the toxicities themselves.

Experimental study on phillyrin resist mice immunosuppression induced by Cyclophosphamide / 中国免疫学杂志

Objective:To study the effect of phillyrin on immune function in mice treated with cyclophosphamide.Methods: Mice model of immunosuppression was induced by intraperitoneal injection of cyclophosphamide,the mice in the experimental group received phillyrin with 50,100 and 150 mg/kg doses by gavage.While model group and control group received normal salin,all treatments continued 7 d.The thymus and spleen organ coefficients was measured by conventional methods.The phagocytosis of peritoneal macrophages was measured by FITC microspheres.The levels of IL-2,IL-4 and cAMP in peripheral blood were detected by ELISA.The effects of different doses of phillyrin on the above indexes were analyzed in immunosuppressed mice.Results: A mice model of immunosuppression was established after 7 d,the results showed that the immune organ index,phagocytic capacity of macrophages and the level of immune factors in serum could be improved,and the content of cAMP in peripheral blood lymphocytes was decreased in different degree by phillyrin.Conclusion: Phillyrin has the function of immune regulation,which can resist the immunosuppression of cyclophosphamide.

Comparison of efficacy and safety of different therapeutic regimens as second-line treatment for small cell lung cancer / 中国基层医药

Objective To discuss the efficacy and safety of different second-line chemotherapies in small cell lung cancer chemotherapy.Methods 170 patients with small cell lung cancer who were failure to first -line treatment were chosen,they were randomly divided into observation group and control group.The control group was given paclitaxel plus cisplatin second-line chemotherapy,the observation group used the irinotecan joint cisplatin second-line chemotherapy.The effect of the two groups was recorded.Results The effective rate of the observation group was 45.88%,which of the control group was 25.88%,the difference between the two groups was statistically significant (χ2 =7.389,P<0.05).The median survival time,total survival time,median disease-free survival time, Karnofsky score,pain score of the observation group were (10.32 ±2.41)months,(7.89 ±1.88)months,(7.54 ± 1.78)months,(83.23 ±6.89)points,(2.37 ±1.07)points,which of the control group were (7.45 ±1.03)months, (3.16 ±0.79)months,(3.89 ±0.68)months,(69.97 ±2.33)points,(4.81 ±2.13)points,the differences between the two groups were statistically significant (t =10.095,21.384,17.660,21.384,17.660,all P<0.05).In the observation group,nausea and vomiting occurred in 5 cases,thrombocytopenia in 3 cases,anemia in 1 case,leukopenia in 7 cases,hypodynamia in 12 cases.In the control group,nausea and vomiting occurred in 16 cases,thrombocytopenia in 11 cases,6 cases of anemia,leukopenia in 18 patients,hypodynamia in 26 cases,the differences between the two groups were statistically significant(χ2 =6.574,4.981,4.722,4.981,4.722,all P <0.05).Conclusion The second-line chemotherapy regimens with irinotecan used in small cell lung cancer patients can improve the treatment effect,adverse reaction mild,prolong patients'survival,it is worth popularizing in clinical.

Clinical effects of Solifenacin on female overactive bladder symptoms secondary to uncomplicated lower urinary tract infection / 实用医学杂志

Objective To study the efficacy and safety of Solifenacin on female overactive bladder (OAB) symptoms secondary to uncomplicated lower urinary tract infection. Methods Seventy-three adult female patients who had clinically diagnosed as OAB symptoms secondary to uncomplicated lower urinary tract infection were randomly divided into treatment group (41 cases) and control group (32 cases). Treatment group received Solifenacin , 5 mg orally qd , as well as OAB behavioral therapy , but control group was given only OAB behavioral therapy. The overactive bladder syndrome score (OABSS) was evaluated before and after antibiotic treatment in all the patients , and the treatment or observation time lasted four weeks after the antibiotic treatment were deactivated. Then OABSS scoring and the cure rate between two groups were compared. Results OABSS score of treatment group decreased significantly after receiving Solifenacin treatment one week , and the cure rate reached 56.10%, and two weeks later, the cure rate reached 92.68%. OABSS score of control group had no significant change one week after treatment and the cure rate was only 6.25%, but at the fourth week OABSS had decreased significantly and the cure rate reached 37.50%, which was still significantly lower than that of treatment group. There were no drug adverse events during treatment in both groups. Conclusions There is obvious clinical effect of solifenacin on female OAB symptoms secondary to uncomplicated lower urinary tract infection, which is safe and could significantly shorten the course of treatment.

Study on CD31 expression in the process of the calf cortical with partial cancellous bone xenograft / 中国医师杂志

Objective To investigate the expression and tissue distribution of CD31 in the process of the calf cortical with partial cancellous bone xenograft, to explore the healing mechanism and relationship between bone remodeling and revascularization, and to provide a basis for the further study of tissue engi-neered bone.Methods Thirty-six New Zealand rabbits were selected as our experimental animals.All ani-mals were implanted with the calf cortical with partial cancellous bone.Specimens were procured at 4, 8, 12 and 24 weeks after surgery, respectively.The expression and tissue distribution of CD31 were observed in the process healing with general condition, X-ray, histology and immunohistochemistry.The image analy-sis computer system was used for quantitative analysis.The extent of revascularization and remodeling was separately represented by revascularization indexes and new bone areas.The relationship was studied be-tween bone remodeling and revascularization.Results ⑴ The X-ray showed significant healing.⑵ The positive expression of CD31 was seen in the whole process of bone xenograft and was different at various stage and sites.By the 4 weeks and 8 weeks postoperative,the strong positive expression was seen in the pe-ripheral soft tissue of graft bone, the callus of joint and the periphery of cartilage nests.Much positive ex-pression was seen in the cancellous bone.There was seen some single, cord, and cluster vascular endotheli-al cells.The positive expression was seen in the enlarged haversian canals and periphery.By the 24 weeks postoperative,the positive expression was seen in the enlarged haversian canals and periphery with increasing newborn capillaries.⑶ The correlation analysis between new bone areas and revascularization showed a closely positive correlation ( r =0.984).Conclusions The positive expression of CD31 was seen in the whole process of bone xenograft and was different at various stage and sites.The positive expression of CD31 reflected the extent of revascularization and bone remodeling of bone grafts.The extent of revascularization of bone grafts and bone remodeling showed a closely positive correlation.

Pulmonary protection of dexmedetomidine during one-lung ventilation in patients undergoing radical operation for lung cancer / 中华麻醉学杂志

Objective To evaluate the pulmonary protection of dexmedetomidine during one-lung ventilation in patients undergoing radical operation for lung cancer.Methods Thirty patients of both sexes,aged 40-70 yr,weighing 50-80 kg,of ASA physical status Ⅰ-Ⅲ,scheduled for elective radical operation for lung cancer,were randomized into 2 groups (n =15 each):dexmedetomidine group (group D) and control group (group C).Before induction of anesthesia,a loading dose of dexmedetomidine 1.0 μg/kg was infused over 10 min,followed by continuous infusion of dexmedetomidine at a rate of 0.6 μg· kg-1 · h-1 until the chest was closed in group D,while the equal volume of normal saline was given in group C.Immediately after induction of anesthesia,at 30 and 60 min of one-lung ventilation,and at the end of operation,arterial blood samples were collected for determination of the serum concentrations of TNF-α,IL-6 and IL-8,and for blood gas analysis.Oxygenation index was calculated.Results Compared with group C,the serum concentrations of TNF-α,IL-6 and IL-8 were significantly decreased,and oxygenation index was increased in group D.Conclusion Dexmedetomidine can mitigate inflammatory responses during one-lung ventilation and improve pulmonary function,thus providing pulmonary protection in patients undergoing radical operation for lung cancer.

Effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation and tumor stem cell CD133 expression / 中国生化药物杂志

Objective To discuss the effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation CD133 expression on tumor stem cell.Methods Hepatocellular carcinoma SMMC7721 cells were cultured,digested and passaged,and divided into six groups with different drug:control group with no daidzein,100 μg/mL daidzein group,200μg/mL daidzein group,300μg/mL daidzein group,400μg/mL daidzein group ,500μg/mL daidzein.The inhibition ratio,hexokinase,alkaline phosphatase and CD133 levels in SMMC7721 cell were detected and compared at 24 h,48 h,72 h among those groups. Results The inhibition ratio was increased by Daidzein dose increasing,and decreased apparently by times extending,especially in 400μg/mL and 500μg/mL daidzeingroups.Compared with control group,the hexokinase and alkaline phosphatase activity and CD133 expression were decreased apparently in groups treated with daidzein(P<0.01).The more the dose,the higher the drop(P<0.01).Conclusion Daidzein can inhibit hepatocellular carcinoma SMMC7721 cell proliferation,and inhibit CD133 expression on tumor stem cell.

Effect of Calcium-sensing Receptor on Human Macrophages Converting to Foam Cells With its Mechanisms / 中国循环杂志

Objective: To study the expression of calcium-sensing receptor (CaR) in human macrophages and the effect of CaR on macrophages converting to foam cells with its mechanism. Methods: Human macrophages were treated with ox-LDL (100 mg/L) for 48 h to induce foam cell formation, the experimental foam cells were cultured in 3 groups for 48 h respectively. Blank control group, Foam cell + CaR agonists (Agonists) group and Foam cell + CaR inhibitor (Inhibitor) group. The CaR expression in macrophages was measured by immuno-fluorescence and Western blot analysis. The positive foam cell formation was detected by oil red O staining, the intracellular cholesterol metabolism in foam cell was observed by HPLC, the inlfammatory cytokine secretion in foam cell culture supernatant was examined by ELISA, the protein expressions of CD36 and acyl-coA: cholesterol acyl-transferase (ACAT-1) in foam cells was measured by Western blot analysis. Results: Immuno-lfuorescence and Western blot indicated CaR is expressed in human macrophages. Compared with Control group, Oil Red O staining and HPLC showed that Agonists group had less positive foam cell formation, decreased CE, FC , TC, and Inhibitor group had more positive foam cell formation, increased CE, FC, TC, allP<0.05; ELISA presented that Agonists group had decreased TNF-α, MIF, increased anti-inflammatory cytokine IL-10, and Inhibitor group had increased TNF-α, MIF, decreased IL-10, allP<0.05; Western blot analysis indicated that Agonists group had decreased protein expressions of CD36, ACAT-1 in foam cells, and Inhibitor group had increased protein expressions of CD36 and ACAT-1. Conclusion: CaR is expressed in human macrophages, the higher CaR expression may inhibit macrophages converting to foam cells.

The effects of venom peptide liquor on adjunctive arthritis in mice / 重庆医学

Objective To investigate effect of the venom peptide liquor on adjunctive arthritis (AA) in mice .Methods The male Kunming mice were randomly divided into 6 groups ,named as the control group ,model group ,positive control group(1 mg/kg dex-amethason) ,wine group(10 mL/kg) ,low dosage of venom peptide liquor group(8 .3 mL/kg) and high dosage of venom peptide liq-uor group(33 .2 mL/kg);the right toes ,ankle diameter and whole body weight were measured at 7-day intervals ;the spleen index , serum level of circulating immune complexes(CIC) were determined after the thirty-fourth day when the mice was put to death .Re-sults The level of CIC in AA mice decreased significantly (P<0 .05) ,the decrease of spleen index and the reduction of toe and an-kle swelling in the low dose group were significant(P<0 .01) .Conclusion The venom peptide liquor exhibited apparent inhibitory effect on AA in mice .

Experimental study of rabbit bone mesenchymal stem cells seeding onto xenogenic calf cortical bone with partial trabecular bone / 中南大学学报(医学版)

OBJECTIVE@#To explore the possibility of xenogenic calf cortical bone with partial trabecular bone as cell carriers in tissue engineering, and to provide direct evidence for clinical application of matrix scaffold.@*METHODS@#Rabbit bone mesenchymal stem cells (BMSCs) were seperated and cultured on the surface of xenogenic calf cortical bone with partial trabecular bone. Relative growth rate was measured and cytotoxicity was graded. The growth, proliferation and matrix secretion of BMSCs in calf cortical strut were observed by scanning electron microscope. Sixty 3-month-old New Zealand rabbits were divided into 3 groups (20 in each group): a BMSCs/bone xenograft group, a simple bone xenograft group and an autogenous iliac bone group. Each group was implanted with the BMSCs induced osteogenic composite calf bone, simple bone xenograft and autogenous iliac bone into the ala of ilium. The expressions of bone morphogenetic protein-2 (BMP-2) mRNA were detected by reverse transcription polymerase chain reaction in 4, 8, 12, 24 weeks after the operation.@*RESULTS@#The toxicity gradation was 0 to 1. BMSCs adhered the surface of calf cortical strut and extended in the cancellous bone, and extracellular matrices were found. The BMP-2 mRNA expression in the simple bone xenograft group was lower than that in the BMSCs/bone xenograft group and the autogenous iliac bone group at all time points (P<0.05),which was lower in the BMSCs/bone xenograft group than that in the autogenous iliac bone group (P<0.05).@*CONCLUSION@#Rabbit BMSCs combined with calf cortical bonescaffold with partial cancellous bone have good osteogenic capacity in the New Zealand rabbits.

Prevention of thoracolumbar osteoporotic compression fracture with transpedicular intracorporeal bone graft with allogenic bone / 中华创伤杂志

Objective To evaluate the clinical efficacy of transpedicular intracorporeal bone graft with allogenic bone in treatment of thoracolumbar osteoporotic compression fracture. Methods A total of 45 patients with thoracolumbar osteoporotic compression fractures were treated with posterior short segment pedicle screw fixation and transpedicular intracorporeal bone graft with allogenic bone. Anteroposte-rior and lateral X-ray photographs were taken before and after operation and at follow up period to determine the ratio of anterior and middle compressed body height to the normal height, the vertebral angle and the superior-inferior endplate angle. The extent of local pain was measured by VAS score. The implant failure was also recorded during follow-up. Results The operative reduction and interbody bone grafting exerted a satisfactory effect on the ratio of anterior and middle body height to the normal height, the vertebral angle and the superior-inferior endplate angle. Local back pain disappeared immediately after surgery in 34 patients out of 38 patients followed up for more than two years. No implant failure was found during follow-up. Conclusions Early treatment with posterior short segment pedicle screw fixation and transpedicular intracorporeal bone graft with allogenic bone can effectively correct local deformity, prevent late vertebral collapse and implant failure and is an ideal treatment method for thoracolumbar osteoporotic compression fracture.

The effect of 5'-nucleotidase from Trimeresurus albolabris venom on platelet aggregation and its mechanism / 中国生化药物杂志

Purpose To investigate the effect of 5'-nucleotidase from Trimeresurus albolabris venom on platelet aggregations and its mechanism.Methods Piatelet aggregations induced by ADP,AA and PAF,respectively.were measured by turbidimetric method after platelet incubated with 5'-nucleotidase.Results 5'-nucleotidase significantly inhibited platelet aggregations induced by ADP,AA and PAF in a dose-dependent manner.Washed platelet inhibition experiment and the effect of Adenosine,CP/CPK on platelet aggregations showed that platelet aggregations inhibited by 5'-nucleotidase were associated with ADP hydrolysis and adenosine accumulation.The effect of ASA on platelet aggregations showed that the enzyme inhibited platelet aggregations probably by blocking the formation of TXA2.Conclusion 5'-nucleotidase from Trimeresurus albolabris venom inhibited platelet aggregations through ADP hydrolysis and adenosine accumulation,and maybe it is related to blocking the formation of TXA2.

Influence of swan-like memory connector on the contents of local insulin-like growth factor Ⅰ during fracture union / 中国组织工程研究

BACKGROUND:Swan-like memory connector (SMC) can form a three-dimensional fixation on long bone shaft,ensure the stability of fracture ends,and produce a continuous dynamic compressive stress on the fracture ends.Insulin-like growth factorⅠ (IGF-Ⅰ) regulates bone development and metabolism,cell differentiation,as well as mitosis.During the union process of fracture,IGF-I is effective to promote cellular proliferation,stimulate bone formation and result in chondrogenesis.OBJECTIVE:To quantitatively determine the effect of SMC and conventional dynamic compression plate (DCP) on the concentration of local IGF-Ⅰ in the union duration of rabbit humerus fracture.DESIGN,TIME AND SETTING:A controlled observational animal study was performed in the laboratory,Department of Orthopaedics,Changhai Hospital,the Second Military Medical University of Chinese PLA from January to May in 2007.MATERIALS:SMC was Ni-Ti alloy panel,comprising 50%-53% Ni,was customized by Lanzhou Seemine Shape Memory Alloy Co.,Ltd.The temperature of shape recovery was (33±2) ℃.METHODS:Thirty adult New Zealand rabbits were used in this study and were processed into osteotomy.The experiment sides of rabbit humerus were cut and fixed with SMC at random,serving as SMC group,and the other sides were cut at the same position and fixed with DCP,serving as DCP group.Each five animals were put to death at the time points of 2,3,4,6,8,and 12 weeks after internal fixation.Specimens were obtained from the areas that were 0.5 cm besides the fracture line.MAIN OUTCOME MEASURES:Following internal fixation,the contents of local IGF-Ⅰ in the specimens of different time points (2,3,4,6,8,and 12 weeks) were determined in both SMC group and DCP group.RESULTS:In the SMC-treated group,the contents of IGF-I increased 2 weeks after internal fixation,reached the maximum at 6 weeks,and then decreased at 8-12 weeks.In the DCP-treated group,the contents of IGF-Ⅰincreased 2 weeks after internal fixation,reached the maximum at 8 weeks,and then decreased at 12 weeks.No significant differences were found between the SMC group and the DCP group at 2,8,12 weeks after internal fixation (P ＞ 0.05).Compared with the DCP group,the SMC group had a remarkably higher content of IGF-Ⅰ at the time points of 3,4 and 6 weeks (P ＜ 0.05).The difference was the most statistically significant at 4 weeks.CONCLUSION:Compared with conventional DCP,the SMC could better promote the secretion of IGF-Ⅰin fracture and facilitate the fracture union due to the continuous dynamic compressive stress and three-dimensional fixation.

Protective effects of minocycline on renal ischemia-reperfusion injury in rats / 重庆医科大学学报

Objective:To investigate the protective effects of minocycline on renal ischemia-reperfusion injury and its mechanism in rats.Methods:72 male Sprague-Dawley rats were randomly divided into three groups:sham-operated(Sham),ischemia-reperfusion(Ir),minocycline hydrochloride(M),for each of the four phases(6h、12h、24h、72h).45 minutes after the left renal artery occlusion on the basis of the right renal resection,we restored perfusion under modeling of ischemia-reperfusion injury.Determined myeloperoxidase(MPO)activity and serum creatinine(Scr),which indicated the extent of the injury for neutrophil infiltration and renal function.Observed renal histological and ultrastructural changes.Results:The level of MPO and Scr was elevated in Ir,and it was distinctly lower in M than that in Ir.Difference between the two groups was of great significance(P

Bioactive peptide from bee venom for adjuvant-induced arthritis in rats / 中国组织工程研究

BACKGROUND: Traditionally, bee venom can treat rheumatic arthritis,rheumatoid arthritis(RA) and so on, but it has strong side effects. So it has been hoped for a long time that the effective angle component could be screened from bee venom, which can be used for the treatment of arthritis perfectly than bee venom.OBJECTIVE: To investigate whether bioactive peptide from bee venom could inhibit infection of arthritis by regulating immunological function so as to probe into a new treatment for RADESIGN: Completely randomized controlled experimental trial based on experimental animalsMETHODS: A municipal key laboratory of animal biology.MATERIALS: The experiment was carried out in the Chongqing Key Laboratory of animal biology from January 2001 to May 2002. Totally 80 rats of clean grade aged 2 to 3 months old with the body mass of 180 to 200 g were provided by Animal Experiment Center of Third Military Medical University of Chinese PLA. The experimental animal certification number was SYXK1 (army) 2002 -007. The animals were divided into 3 groups: normal control group( 10 cases), arthritis group( 10 cases), bioactive peptide group(30 cases).METHODS: Adjuvant-induced arthritis animal models were used and bioactive peptide were given to the animals by muscle injection to observe the knuckle volume and knuckle index changes.MAIN OUTCOME MEASURES: The effect of bioactive peptide from bee venom on the change of knuckle volume and knuckle index in adjuvant-induced arthritis ratsRESULTS: Ten days after injection of 0. 15 mg for each rat, the volume of the paw was (4.72 ±0. 58) mL and the knuckle index was (4.47 ±0.46) mL,which there was significant difference compared with the control group (P＜ 0. 05).CONCLUSION: P-peptide possibly has certain inhibitory effect on the development of the adjuvant-induced arthritis in Wistar rat, and will possibly be a potential therapeutic drug.

Quality Standard of Tongtong Granule / 中国药房

OBJECTIVE: To establish the quality standard of Tongtong granule. METHODS: Panax notoginseng,Ginkgo biloba and Pueraria lobata were identified qualitatively by TLC and the content of total flavonol glyconsides in G. biloba was determined by HPLC. RESULTS: The TLC spots were distinctive and specific. The linear range of quercetin, kaempferide, isorhamnetin were 5.24～83.84 ?g?mL-1(r=0.999 2), 4.56～72.96 ?g?mL-1(r=0.999 1) and 2.48～39.68 ?g?mL-1(r=0.999 4), respectively. The average recoveries were 96.21%(RSD=2.06%)、99.14%(RSD=1.19%)、95.00%(RSD=1.31%),respectively. CONCLUSION:The method is reliable, accurate, and reproducible for the quality control of Tongtong granule.