ABSTRACT
Objective To investigate the infection status and epidemiological characteristics of viral pathogens in hospitalized patients with severe acute respiratory infection (SARI) in Guangdong Province from 2019 to 2021, so as to provide reference for clinical diagnosis and prevention. Methods The respiratory tract samples of SARI patients collected from 2019 to 2021 were detected and analyzed for respiratory syncytial virus (RSV), adenovirus (ADV), human rhinovirus/enterovirus (HRV/EV), human metapneumonic virus (HMPV) and other common respiratory viruses using Luminex respiratory multi-pathogen detection technology. Results A total of 1 948 influenza-negative cases were collected, of which 24.28 % were positive detection of virus infection. HRV/EV was the highest (10.32%), followed by RSV (4.31%). The detection rates were statistically significantly different among different age groups (χ2=176.186，P2=0.042，P>0.05). The detection peaks of RSV were mainly concentrated in summer and autumn, while HMPV was prevalent in winter, and HRV/EV and ADV had no obvious seasonality. Mixed infection was found in 39 samples, and the mixed infection rate was 2.00%. In the mixed infection cases, HPIV and HRV/EV combined infection was the most common. Conclusion HRV/EV, RSV, HMPV and ADV are predominant viral pathogens in SARI influenza-negative hospitalized cases in Guangdong Province from 2019 to 2021. It is recommended to strengthen the surveillance of SARI cases in children under 5 years old.
ABSTRACT
Objective To study the changes in intestinal structure and function of Parkinson's dis-ease(PD)model in cynomolgus monkeys so as to provide scientific basis for exploring the mecha-nism and intervention of constipation and other intestinal symptoms in PD patients.Methods Six male cynomolgus monkeys were subjected,and three of them received intracarotid injection of MPTP for manifestations of typical PD motor symptoms.The monkeys with the Kurlan score reached 10 and persisting for over 3 months without abrogation were identified as PD model(PD group).The other three monkeys served as control group.The brain and ileum tissue samples were collected from the two groups of monkeys.Immunohistochemical staining with tyrosine hydroxy-lase(TH)was used to determine the number of dopaminergic neurons in the substantia nigra of the brain and the changes in peripheral TH of the intestine.HE staining was employed to observe the structure of the intestine.Intestinal permeability was evaluated by atresia occlusive zone pro-tein 1(ZO-1),and intestinal peristalsis function was assessed with intestinal pacemaker cell bio-markers oncogene c-kit and protein gene product 9.5(PGP9.5).Results When compared with the control group,the PD group had significantly less number of nigrostriatal dopaminergic neurons in the left and right sides(133.13±108.63 vs 957.21±225.56,t=5.705,P=0.005;155.74±62.48 vs 917.52±161.14,t=7.611,P=0.002),lower expression of TH in the ileal intermuscular plexus(0.79±0.01 vs 0.73±0.01,t=5.149,P=0.007),reduced villus density with irregular arrange-ment,scattered structure,and detachment of goblet cells,and increased expression of ZO-1(0.28± 0.01 vs 0.22±0.02,P=0.006)and c-kit(0.21±0.01 vs 0.18±0.01,P=0.007)in the ileum muco-sa,but there was no such difference in the expression of PGP9.5 between the two groups(0.31± 0.08 vs 0.38±0.06,P=0.322).Conclusion Intestinal barrier integrity is damaged in PD mon-keys,with damaged villi,increased intestinal permeability and decreased peristalsis,and peristaltic function related intermuscular neurons are not involved.
ABSTRACT
Objective@#To examine the application of arm circumference to evaluating the nutritional risk among cancer patients, so as to provide insights into nutritional risk screening among cancer patients.@*Methods@#Totally 332 cancer patients hospitalized in the Department of Oncology of The First Affiliated Hospital of Guangxi Medical University from September 2020 to March 2021 were selected as the study subjects. Subjects'demographic data and disease history were collected, and the height, body weight, arm circumference and serum nutritional indicators were measured. The indicators related to nutritional risk were identified by logistic regression models. The value of arm circumference in assessment of nutritional risk was examined among cancer patients using the receiver operating characteristic ( ROC ) curve analysis, and the Nutritional Risk Screening ( NRS 2002 ) scores were used as the gold standard.@*Results@#The subjects included 188 males ( 56.63% ) and 144 females ( 43.37% ), and had a mean age of ( 51.62±12.31 ) years. The detection rate of nutritional risk was 36.75% among the subjects according to NRS 2002, with 29.78% in males and 45.83% in females. Multivariable logistic regression analysis identified arm circumference as an independent factor affecting the nutritional risk among cancer patients ( P<0.05 ). The area under the ROC curve, cut-off, sensitivity and specificity of arm circumference in predicting nutritional risk were 0.857 ( 95%CI: 0.795-0.918, P<0.001 ), 24.4 cm, 83.3% and 78.6% among male cancer patients, and 0.727 ( 95%CI: 0.643-0.810, P<0.001 ), 23.9 cm, 78.2% and 57.6% among female cancer patients, respectively.@*Conclusions@#Arm circumference is feasible for screening nutritional risk among cancer patients. The cut-off value of nutritional risk was determined by arm circumference less than 24.4 cm in men and less than 23.9 cm in women with good accuracy.
ABSTRACT
With the rapid improvement of the perception and computing capacity of mobile devices such as smart phones, human activity recognition using mobile devices as the carrier has been a new research hot-spot. The inertial information collected by the acceleration sensor in the smart mobile device is used for human activity recognition. Compared with the common computer vision recognition, it has the following advantages: convenience, low cost, and better reflection of the essence of human motion. Based on the WISDM data set collected by smart phones, the inertial navigation information and the deep learning algorithm-convolutional neural network (CNN) were adopted to build a human activity recognition model in this paper. The K nearest neighbor algorithm (KNN) and the random forest algorithm were compared with the CNN network in the recognition accuracy to evaluate the performance of the CNN network. The classification accuracy of CNN model reached 92.73%, which was much higher than KNN and random forest. Experimental results show that the CNN algorithm model can achieve more accurate human activity recognition and has broad application prospects in predicting and promoting human health.
Subject(s)
Humans , Algorithms , Cluster Analysis , Human Activities , Motion , Neural Networks, ComputerABSTRACT
Objective To rkskarch,dkvklop and vkrife of a simplk scrkkning path for 4-6 ekar old childrkn＇s sknsore intkgration. Methods Nink hundrkd and thirte-six childrkn agkd 4-6 ekars wkrk invkstigatkd be stratifikd clustkr random sampling from Octobkr to Novkmbkr of 2017,of whom 476 wkrk boes and 460 wkrk girls. L sknsore intk-gration rating scalk(Childrkn＇s Edition)was uskd to asskss thk sknsore intkgration abilite,and thk main kntre construc-tion profilk scrkkning scalk was kxtractkd according to thk factor analesis,and thk critical valuk was dktkrminkd in com-bination with thk arka undkr thk subjkct opkrating charactkristic curvk( AOC),thk rkliabilite,validite,sknsitivite and spkcificite wkrk kvaluatkd. ResuIts Thk sknsore intkgration disordkr ratk of 4-6 ekar old childrkn was 47. 76﹪,and thk 14 main factors wkrk kxtractkd be factor analesis,and thk cumulativk contribution ratk was 75. 052﹪. Lftkr thk max-imal orthogonal rotation of varianck,thk itkms with factor load matrix cokfficiknt ≥0. 75 wkrk sklkctkd to construct thk simplk sknsore intkgration scrkkning scalk,which wkrk simplifikd to 2 classification options from 13 itkms in thk original tablk. Thk sknsitivite of thk simplk scrkkning scalk was 82. 77﹪,and thk spkcificite was 80. 16﹪,and thk arka undkr thk AOC curvk was 0. 815. Thk corrklation cokfficiknts of vkstibular balanck,propriockption,tactilk sknsation,visual smoothnkss and auditore rkcognition with thk summare tablk wkrk 0. 793,0. 372,0. 699,0. 604,0. 482,rkspkctivkle( all P﹦0. 000). Thk Kappa of thk summare tablks and gknkrad tablk was 0. 628(P﹦0. 000). Thk Cronbach＇s Llpha of thk summare tablk was 0. 767. ConcIusions Thk simplk scrkkning scalk simplifiks thk sknsore intkgration rating scalk into skvkral main itkms,which is simplk to opkratk and can bk uskd as a primare scrkkning mkthod for sknsore intkgration disordkr.
ABSTRACT
Objective To investigate the interaction between rs1800955 polymorphism of dopamine D4 receptor(DRD4) gene and negative life events on personality characteristics of Mongolian adolescents.Methods A total of 239 Mongolian adolescents aged 12-15 were assessed with Eysenck Personality Questionnaire(EPQ) and Adolescent Self-Rating Life Events Check List(ASLEC).The polymorphism of DRD4 gene rs1800955 was determined by improved multiple ligase detection reaction(iMLDR) technique.Results (1) The rs1800955 polymorphism of DRD4 gene was significantly correlated with psychoticism score of EPQ.The psychoticism score of individuals with CC genotype (4.94 ± 3.19) was higher than that of TT genotype (3.38±2.29),and the difference was statistically significant (P＜0.05).(2) The scores of psychoticism and neuroticism in Mongolian adolescents were positively correlated with the factors of negative life events(r=0.154-0.375,P＜0.05 or 0.01).(3) The interaction between the rs1800955 polymorphism of DRD4 gene and negative life events significantly affected the scores of psychoticism in Mongolian adolescents (C C genotype x interpersonal factor:B =-2.689,95 ％ CI =-4.589--0.789,x2 =7.695,P＜ 0.01).In individuals with CC genotype,the scores of psychoticism in those with high scores of interpersonal relatioriship factors were significantly higher than those with low scores of interpersonal relationship factors ((3.01 ± 0.71) vs (2.61 ±0.67);t =-3.066,P＜ 0.01).Conclusion The polymorphism of DRD4 gene rs 1800955 and its association with interpersonal factors play an important role in the psychoticism of Mongolian adolescents.The CC genotype is a risk factor of psychoticism,and the poor interpersonal relationship may increase the risk of individuals with CC genotype.
ABSTRACT
Objective@#To understand the depression of nurses in the maternity and child care hospital and in order to provide references for improving the physical and mental health of nurses.@*Methods@#A total of 679 nurses from 14 departments of a provincial maternity and child health care hospital in June 2017 were selected as the subjects to investigate the depression of nurses by questionnaire.@*Results@#The total rate of depression was 40% in this group and the average standard of SDS was (49.45+12.02) , which was higher than the norm in our country and the difference was statistically significant (P<0.01) . The average standard of SDS for nurses in different departments was higher than the norm in our country except the women's health caredepartment. The differences of the depression in age、the age for nurse、monthly income、night shift frequency、children's status、the work feelings and family support were statistically significant (The values of 2 are 12.908、9.953、10.316、19.422、11.246、41.672 and 61.434, P<0.05) . Multivariate Logistics Regression Analysis showed age、family income、night shift frequency、the work feelings、family support were risk factors of depression among nurses, by 26-35 years of age、the monthly income less than 6 000 yuan、night shift frequency (less than 5~6 days) , dislike work、family support for work in general and no support.@*Conclusion@#The rate of depression among nurses in provincial maternity and child health care hospital is high. Hospital departments should attach great importance to the psychological problems of nurses and give psychological intervention in order to improve the mental health level of nurses.
ABSTRACT
Objective To explore the association between the cholesterol level and disease relapse in patients with Neuromyelitis Optica Spectrum Disorders (NMOSD). Methods Clinical and biochemical data of 96 patients with NMOSD were retrospectively analyzed. According to disease relapses, NMOSD patients were divided into primary and relapse groups.Their clinical characteristics and cholesterol level were compared between the two groups.The correlation between cholesterol level and disease recurrence was analyzed by partial correlation adjusted for sex. Results Between the primary group and relapse group,there were statistically significant differences in gender(48.8% vs. 80%, P<0.05), cholesterol (CHO)(4.27±0.85 vs. 5.18±1.26)and low density lipoprotein cholesterol (LDL-C)level[2.37(0.90)vs. 3.00 (1.21)](P<0.001). There were no significant difference in age, upper respiratory infection, gastrointestinal tract, rate of higher cerebrospinal fluid protein, triglyceride (TG)and high density lipoprotein cholesterol(HDL-C)(P>0.05). The percentage of recurrent patients in CHO normal and higher groups were 43.55% and 82.35% respectively, which was statistically significant difference between the two groups ( x2=13.51, P<0.01); The rate of relapse of LDL-C normal and higher groups were 47.69% and 75% respectively, which was statistically significant difference between the two groups ( x2=7.58,P<0.01).After adjusting for sex,CHO level was positively correlated with disease relapse(r=0.346,P<0.01),and LDL-C level also was positively correlated with disease relapse(r=0.380,P<0.01). Conclusion High CHO and LDL-C level may be associated with disease relapse, which has some clinical guiding significance for controlling CHO level in NMOSD patients.
ABSTRACT
Objective To evaluate the efficacy and safety of a superpulse-mode fractional carbon dioxide(CO2) laser for the treatment of onychomycosis. Methods Patients with typical clinical manifestations of onychomycosis and positive for direct microscopic examinations of fungi were enrolled into this study, and treated with a superpulse-mode fractional CO2 laser for eight sessions. The scoring clinical index for onychomycosis (SCIO)and onychomycosis severity index (OSI)were calculated according to patients′ age, clinical type of onychomycosis, thickness of nails, area and length of nail involvement before the treatment, at the end of treatment, 1 month and 3 months after completion of treatment. Mycological clearance was also evaluated according to direct microscopy and fungal culture results. Adverse reactions to laser therapy were recorded. Statistical analysis was carried out by using the chi-square test and Wilcoxon signed-rank sum test with the SPSS 17.0 software. Results Totally, 20 patients with onychomycosis were enrolled into this study, and 75 affected nails were treated. Finally, 18 patients with 71 target nails completed the treatment and follow-up. The SCIO and OSI were 13.07 ± 6.47 and 21.11 ± 11.94 in these patients at baseline respectively, both significantly different from those at the end of treatment(9.03 ± 6.14 and 13.63 ± 12.10, respectively, both P 0.05). The SCIO and OSI decreased from 12.48 ± 5.41 and 16.44 ± 9.89 at the baseline to 5.01 ± 5.56 and 6.44 ± 8.26 at 3 months after the treatment, respectively, in patients with distal and lateral subungual onychomycosis (DLSO), and from 17.86 ± 3.98 and 34.05 ± 2.56 to 15.88 ± 4.10 and 31.00 ± 7.28 respectively in patients with total dystrophic onychomycosis (TDO). During the treatment, several patients felt transient mild pain, but no subungual hemorrhage or other adverse reactions occurred. Conclusions The fractional CO2 laser in superpulse mode shows a reliable efficacy for the treatment of mild to moderate onychomycosis such as DLSO, especially when the nail plate is superficially invaded and grows rapidly. It directly inhibits and kills fungi, and treatment duration should be prolonged according to conditions.
ABSTRACT
Objective:Preparation and immune characteristic analysis of polyclonal antibody against hypervariable region protein of Taura syndrome virus major capsid protein VP 1 as a reference for studies on immunological diagnosis reagent.Methods:The recombinant vector pET-VP1 was transformed into E.coli BL21 for protein expression.Immunizing a New Zealand rabbit with purified VP1 protein,the titer of anti-VP1 serum was determined by Agar diffusion test and ELISA.Monoclonal phage specific binding to the purified VP1 protein was used for competitive inhibition test.Results: The VP1 protein was soluble and high expression in E.coli BL21.The biological activity titer of anti-VP1 serum reached 1∶26 ,1∶217 determined by Agar diffusion test and ELISA respectively.A litter binding activity of antiserum and VP 1 protein could be blocked by monoclonal phage , but would not affect the final positive result.Conclusion:High titer antibody Preparation of the VP 1 hypervariable region protein.The binding activity of the polyclonal antibody with VP1 protein was not affected by the mutations of VP 1 protein in minority areas ,so the antiserum could be used as immu-nological detection diagnosis agent.
ABSTRACT
Objective To explore the efficacy of triple therapy and sequential therapy in the eradication of Helicobacter pylori (Hp) in patients receiving long-term non-steroidal antiinflammatory drugs (NSAID) treatment.Methods Patients receiving long-term NSAID treatment were enrolled in this study.Patients diagnosed as Hp infection were divided into triple therapy and sequential therapy groups.The patients in triple therapy group received omeprazole,clarithromycin and amoxicillin theray for 10 days.The patients in sequential group received esomeprazole with amoxicillin for five days,and then esomeprazole with clarithromycin and metronidazole for another five days.All patients were given mucosal protective therapy as maintenance treatment after eradication therapy and followed up for 12 weeks.Patients underwent endoscopy examination and Hp testing before and after follow-up.Hp eradication rates were compared with the intention-to-treat (ITT) and per protocol (PP) analysis.Results According to ITT analysis,the eradication rates of Hp in triple therapy group and sequential therapy group were 78.4 ％ (40/51) and 80.0 ％ (40/50) respectively,there was no significant difference between these two groups (x2 =0.038,P=0.846).According to PP analysis,the eradication rates of Hp in triple therapy group and sequential therapy group were 84.4％ (38/45) and 87.0％ (40/46) respectively,there was no significant difference between these two groups either (x2=0.117,P=0.732).Conclusion There was no significant difference in Hp eradication between triple therapy and sequential therapy in patients receiving long-term NSAID treatment.
ABSTRACT
Objective To explore the risk factors of and the influence of different hepatitis B virus (HBV) DNA load on paternal vertical transmission of HBV.Methods Totally,161 HBsAg negative women,whose husband was HBsAg positive,attended the antenatal clinics of the Provincial Maternity and Child Health Hospital of Fujian from September 2007 to December 2008 and their newborns were selected,and the epidemiologic information,the duration of being a HBV carrier,the first class HBV family history of the fathers,HBV markers,HBV DNA load,HBsAb of the gravidas,the outcomes of the newborns were all collected.Cord blood was sampled after delivery for HBV DNA quantification and those with HBV DNA load ≥1.0×103 copy/ml were chosen as the case group and those ＜ 1.0×103 copy/ml as control.Results (1) Among the 161 newborns,36 HBV DNA positive cord blood samples were detected,giving a rate of 22.4% (36/161) for paternal vertical transmission of HBV.The HBV DNA positive rate in cord blood was 32.0% (23/72) in HBeAg-positive fathers and 14.6% (13/89) in HBeAg-negative fathers.(2) Univariate analysis showed that HBeAg-positive,HBV DNA positive,first class family history of HBV and the duration of being a HBV carrier of the fathers were risk factors of paternal HBV vertical transmission[X2= 6.892,29.916,29.499 and 23.821,OR = 2.7,5.2,8.3 and 1.4 (P＜0.01)].(3) Multivariate analysis found that paternal serum HBV DNA positive and the first class family history of HBV of the father side were risk factors of paternal vertical transmission of HBV (OR = 11.1,95% CI;4.6-27.1;OR = 17.1,95% CI:3.5-82.6).(4) According to the different serum HBV DNA load of the HBsAg-positive father,7 groups were divided.A dose dependent effect was found that the HBV DNA positive rate of the cord blood increased with the rising of HBV DNA load.No HBV DNA positive cord blood was detected when paternal HBV DNA load was＜1.0×104 copy/ml,while 100% of the cord blood were positive when paternal HBV DNA load≥1.0×108 copy/ml.(5) The average birth weight of the newborns in the two groups was the same (3.3±0.4) kg.And the delivery mode,gestational age at delivery,height and Apgar score of the newborns at 1 minute,neonatal pathological jaundice and other complications had no significant difference between the two groups (P ＞ 0.05).No relationship was found between the neonatal outcomes and the paternal HBV vertical transmission (P＞0.05).Conclusions HBV DNA load in the serum of HBsAg-positive father,and the paternal first class family history of HBV are risk factors of paternal HBV vertical transmission.When the serum HBV DNA load in HBsAg-positive father is≥1.0×107 copy/ml,the possibility of paternal vertical transmission of HBV would increase.
ABSTRACT
Aim Telomerase is highly expression in most tumor cells, and it is an ideal target for cancer molecular targeting therapy. It has been proved that wogonin effectively inhibits telomerase activity and tumor cell growth in vitro. The study was to explore the inhibitory effect of wogonin on the growth of tumor and telomerase activity of implanted human ovarian cancer cell line SKOV3 in nude mice. Methods Nude mice with implanted human ovarian cancer cells SKOV3 were randomly divided into five groups, viz. the high dose group of Wogonin(600 mg?kg-1),low dose group of Wogonin(300 mg?kg-1),normal control group, cisplatin therapy group(3 mg?kg-1), and combined therapy group(cisplatin plus wogonin).The weight of nude mice and the volume of tumor were regularly measured. DNA、RNA and protein were extracted from the tumor tissue. The length of telomere was examined by Southern blot. The expression of telomerase hTERT gene was detected by RT-PCR. The telomerase activity was examined by TRAP-PCR-silver staining. Results The wogonin significantly inhibit the growth of tumor when compared with controlled group.The inhibitory rate of high dose group and low dose group were 56.67% (P=0.002) and 38.10%(P=0.019), respectively. The inhibition rate of cisplatin therapy group was 50.83%(P=0.004). The suppress rate of combined group reached 66.9% and higher than any single therapy(P=0.002). The length of telomere in different concentration groups of wogonin was the same as that in the control group.Wogonin inhibited the expression of telomerase gene hTERT and telomerase activity. The inhibition is related to the dose of wogonin. Conclusion Wogonin suppresses the growth and telomerase activity of tumor. The inhibitory effect is related to the dose of wogonin. Combination of wogonin and cisplatin increase the inhibitory rate in nude mice tumor.
ABSTRACT
Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.