ABSTRACT
<p><b>OBJECTIVE</b>To provide right time points in selection of right aged animals and the normal physiological data of TX mice.</p><p><b>METHODS</b>7-12 months old TX and DL mice were studied, each group contained 3 female and 3 male mice of TX or DL mice. The concentration of copper in the serum, dry tissues (liver, brain and kidney), together with copper biochemistry indexes were measured. The liver histopathology was observed under light microscopy and electron microscope.</p><p><b>RESULTS</b>Transaminase increased significantly only in 10 and 11-month- old (AST(TX10) = 218.3 U/L, AST(TX11) = 197.5 U/L, AST(DL10) = 171.5 U/L, AST(DL11) = 165.0 U/L, P(10) less than 0.001, P(11) = 0.022), but the copper concentration of liver, brain and kidney was significantly increased during 7-12 month old (the average concentration of copper, Liver(TX) = (750.0 +/- 85.5) mg/kg, Brain(TX) = (39.7 +/- 2.2)mg/kg, Kidney(TX) = (29.8 +/- 5.0) mg/kg, Liver(DL) = (11.6 +/- 1.5) mg/kg, Brain(DL) = (16.8 +/- 0.9) mg/kg, Kidney(DL) = (14.2 +/- 1.0) mg/kg, t = 21.16, 23.60, 7.47, for all these organs P less than 0.05).</p><p><b>CONCLUSION</b>TX mice is a suitable model of liver disease with natural recovery, so selecting animal model of suitable time point is very important.</p>
Subject(s)
Animals , Female , Male , Mice , Aspartate Aminotransferases , Blood , Brain , Metabolism , Ceruloplasmin , Metabolism , Copper , Metabolism , Disease Models, Animal , Kidney , Metabolism , Liver , Metabolism , Pathology , Liver Diseases , Blood , Metabolism , Pathology , Mice, Inbred Strains , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To find out the relationship between mutation of ATP7B gene promoter region and pathogenesis of Wilson disease(WD).</p><p><b>METHODS</b>Two of 48 WD patients presented C-->T base substitution mutations at the position -183. DNA sequences of the promoter region from normal and mutant samples were separated. The fragments containing the promoter region were cloned upstream of the luciferase. Luciferase activity was analyzed.</p><p><b>RESULTS</b>The luciferase activity of reporter gene containing normal sequence of ATP7B gene promoter region did not show significant difference as compared with that of reporter gene containing mutant promoter(n=3, P > 0.05).</p><p><b>CONCLUSION</b>No influence of C-->T base substitution mutations on the activity of promoter was observed in study. The results suggest that WD pathogenesis relates little to the mutations of the promoter region in Chinese.</p>
Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Young Adult , Adenosine Triphosphatases , Genetics , Base Sequence , Cation Transport Proteins , Genetics , Cell Line, Tumor , Copper-Transporting ATPases , DNA Mutational Analysis , Hepatolenticular Degeneration , Genetics , Luciferases , Genetics , Metabolism , Mutation , Promoter Regions, Genetic , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To study the strategy of applying molecular genetic methods and techniques in the diagnosis of spinocerebellar ataxias (SCA).</p><p><b>METHODS</b>This study included 43 patients with SCA from 36 families, 38 sporadic SCA patients, 60 healthy individuals from the SCA families and 44 normal controls. The trinucleotide repeats were detected by polymerase chain reaction (PCR), denaturing polyacrylamide gel electrophoresis and silver staining technique. The repeat numbers were calculated by software.</p><p><b>RESULTS</b>SCA3 was the most common type in the Hans of south China, accounting for 42.0%, followed by SCA2 (7.4%), SCA1 (4.9%), SCA7 (3.7%), SCA6 (2.5%) and SCA12 (1.2%). No patient was found to have SCA8, SCA10, SCA17, and dentatorubro-pallidoluysian atrophy(DRPLA).</p><p><b>CONCLUSION</b>Molecular genetic detection is an effective way to confirmation of SCA subtype diagnosis and presymptomatic genetic diagnosis.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Electrophoresis, Polyacrylamide Gel , Pedigree , Polymerase Chain Reaction , Spinocerebellar Ataxias , Diagnosis , Genetics , Trinucleotide Repeats , GeneticsABSTRACT
Objective To study the effects of angiotensin converting enzyme inhibitor benazepri1 on apoptosis and the expression of Fas and FasL in the kidney of rats with adriamycin-indued nephritic glomeruosclerosis.Methods After uninephrectomy and the injection of adriamycin induced rats model with glomerulosclerosis, benazapril(6 mg/kg) was delivered daily by gavage to the rats in therapeutic groups for 12 weeks.Apoptosis was examined by means of terminal-deoxynucleotidyl trans ferase mediated d-UTP nick end label ling(TUNEL) and immunohistochemistry was utlized to detect the expression of Fas and FasL.Software of pathological analysis quantitated the level of Fas and FasL.Results Compared with those of the control group, the kidney of model group had moresevere glomerulosclerosis, much more apoptotic cells and higher level of exprssion of Fas and FasL. The degree of glomeruloscleroais, the nuxner of apoptotic cells and the level of expression of Fas and FasL were ameliofated by benazepril treatment.Conclusion Benazepril may suppress the excessive apoptosis of kidney cell by lowering the expression of the protin correlatng apoptosis Fas and FasL,so as to postpone the process of glomeruosclerosis.