ABSTRACT
Establishment of excellent courses in colleges and universities plays a positive role in building harmonious campus.Building excellent courses helps to foster students' good learning attitude,to improve the young teachers' teaching methods and teaching ability so as to improve teaching quality.It also helps to deepen teaching reform,to promote an increase in the level of scientific research.It pushes towards mutual promotion and coordinated development between teaching and scientific research,with a view to achieving the aim of building all-round harmonious campus.
ABSTRACT
Objective To detect the dynamic changes of antibody and cytokines in the serum induced by single vaccine delivery system (SVDS) of biodegradable microspheres of recombinant glutathione-S-transferase from Schistosoma japonicum (rSjGST) in mice. Methods By using the constructed expression plasmid pET28a(+)-SjGST, rSjGST was expressed by E.coli BL21. After purified through the column of resin,the biodegradable microspheres of rSjGST were prepared with PLGA (Poly lactic-co-glycolic acid, PLGA 75/25).Six-week BALB/c female mice were immunized subcutaneously with a single vaccine, and the blood was collected from eyes on the 6th, 9th, 12th, 15 th, 18th, 21th week respectively. The dynamic levels of serum specific antibody IgG, IL-2 and IFN-? were detected. Results Compared with the control group, there was a significant difference of serum specific antibody IgG of every immune group (P