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Objective To investigate the correlation between the features of conventional ultrasound& shear wave elasticity and axillary lymph node involvement in breast cancer . Methods A total of 169 breast cancers patients were divided into lymph node metastasis group( n = 115) and non metastasis group ( n = 54 ) according to the postoperative pathological results . Preoperative conventional ultrasonographic features and preoperative shear wave elastography quantitative parameters ( E values ) of the two groups breast lessons were analyzed by single factor analysis to screen out statistically significant factors ,then Logistic regression analysis was performed to analyze the relationship between above factors and lymph node involvement . Results Single factor analysis showed the microcalcification and hyperechoic halo detection rates of lymph node metastasis group [ 81 .7％ ( 94/115) and 71 .3％ ( 82/115 ) ,respectively] were higher than those in non metastasis group [ 61 .1％ (33/54) and 50 .0％ ( 27/54) ,respectively] . The elastography maximum value( Emax) of lymph node involvement group was ( 182 .2 ± 74 .0) kPa ,which was larger than that in non metastasis group′s ( 153 .3 ± 76 .9) kPa ( P < 0 .05) . Multivariate Logistic regression analysis showed the microcalcification( OR = 2 .498 , P = 0 .022) ,the hyperechoic halo( OR = 2 .482 , P = 0 .013) and the Emax value( OR = 1 .007 , P = 0 .007) were risk factors of axillary lymph node metastasis in breast cancer . Conclusions Breast cancer with microcalcification ,hyperechoic signs and high Emax value is more likely to develop axillary lymph node metastasis .
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<p><b>OBJECTIVE</b>To investigate the effect of microRNA-99a-5p (miR-99a-5p) on differentiation ability of human bone marrow mesenchymal stem cells (BM-MSC).</p><p><b>METHODS</b>BM-MSC was cultured and then transfected with miR-99a-5p mimics or inhibitors. The transfection efficiency was detected by real-time quantitative PCR. The effects of miR-99a-5p on the adipogenic and osteogenic differentiation ability of BM-MSC were detected by differentiation experiment.</p><p><b>RESULTS</b>As compared with the negative control group, the expression of miR-99a-5p was significantly up-regulated after transfection with miR-99a-5p mimics(P<0.001), the expression of miR-99a-5p was down-regulated after transfection with miR-99a-5p inhibitor (P<0.001). In osteogenic differentiation experiments, the miR-99a-5p overexpression could promote the osteogenic differentiation, while the downregulation of miR-99a-5p expression inhibited the osteogenic differentiation. The same results were obtained by semi-quantitative detection through spectrophotometry. In the adipogenic differentiation test, transfection of miR-99a-5p mimics or inhibitors had no significant effect on the adipogenic differentiation of BM-MSC.</p><p><b>CONCLUSION</b>Overexpression of miR-99a-5p can promote the osteogenic differentiation of BM-MSC, but no significant effects are observed in the adipogenic differentiation.</p>
Subject(s)
Humans , Bone Marrow Cells , Cell Differentiation , Cells, Cultured , Mesenchymal Stem Cells , MicroRNAs , OsteogenesisABSTRACT
Objective To investigate the cuttlebone and calcined gypsum comfeel dressing treatment of diabetic foot results and provide reference basis for measures to explore the diagnosis and treatment of diabetes foot selection methods in January 2016 to March 2017 in our hospital 80 casesⅠ-Ⅲ diabetic foot patients hospitalized with, take meter method is divided into trial group and control group randomly 40 cases, all patients were diagnosed with type 2 diabetes. Two groups based on diabetic foot ulcers qing disinfection comprehensive treatment, treatment group give cuttlebone and calcined gypsum with comfeel dressing therapy, the control group simply give comfeel dressing treatment, the result of the two groups to compare the effect of diabetic foot patients with diabetic foot treatment after 15 days, the experimental group cure the total effective rate 85.00%, control group total effective rate 47.50%, two groups of treatment was significant difference.
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Objective To investigate the cuttlebone and calcined gypsum comfeel dressing treatment of diabetic foot results and provide reference basis for measures to explore the diagnosis and treatment of diabetes foot selection methods in January 2016 to March 2017 in our hospital 80 casesⅠ-Ⅲ diabetic foot patients hospitalized with, take meter method is divided into trial group and control group randomly 40 cases, all patients were diagnosed with type 2 diabetes. Two groups based on diabetic foot ulcers qing disinfection comprehensive treatment, treatment group give cuttlebone and calcined gypsum with comfeel dressing therapy, the control group simply give comfeel dressing treatment, the result of the two groups to compare the effect of diabetic foot patients with diabetic foot treatment after 15 days, the experimental group cure the total effective rate 85.00%, control group total effective rate 47.50%, two groups of treatment was significant difference.
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<p><b>OBJECTIVE</b>To investigate the possible effect of MicroRNA-214 (miR-214) on migration of umbilical cord blood CD34hematopoietic stem/progenitor cells induced by BM-MSC.</p><p><b>METHODS</b>After transfection of the inhibitor or mimic of miR-214, the cultured supernatant of BM-MSC were collected respectively. The expression of miR-214 in BM-MSC was detected by real time quantitative PCR, the effect of supernatant on CD34cell migration was evaluated by chemotaxis assays. The levels of chemokine(SDF-1) secreted by BM-MSC in the supernatant were detected by ELISA.</p><p><b>RESULTS</b>The cultured supernatant of BM-MSC could promote the migration of CD34cells. Compared with the group without transfection or negative control(NC) group, the transfection with miR-214 mimic could promote the migration of CD34cells (P<0.01), while the migration rate in miR-214 inhibitor groups decreased significantly (P<0.01). Further study found that the concentration of SDF-1 was not changed notably in all groups (P>0.05), as compared with the control group.</p><p><b>CONCLUSION</b>miR-214 signalings may indirectly increase the migration of CD34hematopoietic stem/progenitor cells by modulating BM-MSC functions, which may not significantly correlate with the chemokine SDF-1 secreted by BM-MSC.</p>
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<p><b>OBJECTIVE</b>To explore the effect of MicroRNA-146a (miR-146a) on the ability of BM-MSC to differentiate into adipocytes and osteoblasts.</p><p><b>METHODS</b>BM-MSC were isolated from the bone marrow of healthy donors. The differentiation of BM-MSC into adipocytes and osteoblasts cells were done in vitro. After transfection with miR-146a inhibitor or mimics, the expression of miR-146a in BM-MSC was detected by real time quantitative PCR. The effect of MicroRNA-146a on the differentiation potential of BM-MSC was evaluated after transfection.</p><p><b>RESULTS</b>BM-MSC possessed the ability to differentiate into adipocytes and osteoblasts cells when cultured in the induction medium. The expression of miR-146a was correspondingly down-regulated and up-regulated in BM-MSC after transfection. Compared with the control group, the expression of miR-146a was down-regulated (P < 0.01) after transfection with miR-146a inhibitor, while after transfection with miR-146a mimics it was significantly up-regulated. This study proved that the transfection with miR-146a inhibitor can inhibit BM-MSC differentiate into adipocytes (P < 0.01), while transfection with miR-146a mimics can promote differentiation of BM-MSC into adipocytes (P < 0.01). No effect of miR-146a inhibitor or miR-146a mimics on osteogenic differentiation of BM-MSC was observed (P > 0.05).</p><p><b>CONCLUSION</b>BM-MSC possess the ability to differentiate into adipocytes and osteoblasts. The miR-146a can promote BM-MSC to differentiate into adipocytes.</p>
Subject(s)
Humans , Adipocytes , Cell Biology , Bone Marrow Cells , Cell Biology , Cell Differentiation , Cells, Cultured , Mesenchymal Stem Cells , Cell Biology , MicroRNAs , Metabolism , Osteoblasts , Cell Biology , Osteogenesis , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To study the expressions of tumor necrosis factor alpha-induced protein 3(TNFAIP3) and mammary serine protease inhibitor (Maspin) in the radiotherapy of nasopharyngeal carcinoma and explore the differences in radiosensitivity and radioresistance,the relation with the occurrence and development of radioresistance.</p><p><b>METHODS</b>The TNFAIP3 and Maspin mRNA expressions were detected by using TNFAIP3 and Maspin multi-point labeled DIG probes in situ hybridization.</p><p><b>RESULTS</b>In radiosensitivity and radioresistance of nasopharyngeal carcinoma,the moderately and strongly positive TNFAIP3 mRNA expression rates were 27.50% and 48.33% (P=0.037), and the moderately and strongly positive Maspin mRNA expression rates were 67.50% and 46.67% (P=0.040). In the radioresistance of nasopharyngeal carcinoma,TNFAIP3 mRNA moderately and strongly positive expressions were positively correlated with TNM stage (P=0.005). In distant metastasis and no distant metastasis (70.00% and 37.50%, P=0.018), the expression rates had statistical significance. The Maspin mRNA moderately and strongly positive expressions were positively correlated with TNM stage (P=0.039) and T stage (P=0.021). In distant metastasis and no distant metastasis (65.00% and 37.50%, P=0.044), the expression rates had statistical significance.</p><p><b>CONCLUSION</b>TNFAIP3 may be involved in the development of radioresistant nasopharyngeal carcinoma,and Maspin may be related with the invasion and metastasis of radioresistant nasopharyngeal carcinoma.</p>
Subject(s)
Humans , Carcinoma , DNA-Binding Proteins , In Situ Hybridization , Intracellular Signaling Peptides and Proteins , Nasopharyngeal Neoplasms , Nuclear Proteins , Serine Proteinase Inhibitors , Tumor Necrosis Factor alpha-Induced Protein 3ABSTRACT
Background and purpose:Nasopharyngeal carcinoma is sensitive with radiotherapy, and it is of great signiifcance to study the resistance mechanism. This study aimed to analyze the expression of TNFAIP3 in the radiotherapy of nasopharyngeal carcinoma, the difference between radiosensitivity and radioresistance, the relationship with the occurrence and development of radioresistance.Methods:Detection of TNFAIP3 mRNA expression was carried out by TNFAIP3 multi-point labeled DIG probein situ hybridization.Results:In radiosensitivity and radioresistance of nasopharyngeal carcinoma, the moderately and strongly positive TNFAIP3 mRNA expression rates were 15% and 45%, (χ2=5.274,P=0.021 6), there were statistical significance. In the radioresistat nasopharyngeal carcinoma, TNFAIP3 mRNA moderately and strongly positive expression was positively correlated with TNM stage. In the degree ofⅢ-Ⅳ tumor, it had moderately and strongly positive expression rate of 48.28%.In the degree ofⅠ-Ⅱtumor, it had moderately and strongly positive expression rate of 36.36% (χ2=33.240,P<0.005); The expression rates in distant metastasis and no distant metastasis were 81.81% and 31.03% (χ2=6.385,P=0.011 5), the difference had statistical signiifcance.Conclusion:TNFAIP3 has antitumor effect, and it is correlated with the occurrence and development of radioresistant in nasopharyngeal carcinoma.
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Objective:To identify proteins associated with nasopharyngeal carcinoma (NPC) metastasis,and provide scientific basis for the prevention and cure of NPC.Methods:A two-dimensional gel electrophoresis and mass spectrometry were performed to screen for differential proteins between highly metastatic 5-8F and non-metastatic 6-10B NPC cell lines.Western blot was used to confirm the differential proteins.We used siRNA to inhibit the expression of differential protein nm23-H1 to determine the association of nm23-H1 with NPC in vitro invasive ability.Immunohistochemistry and statistics were used to evaluate the correlation of nm23-H1 expression with clinicopathological features and clinical outcomes in paraffin-embedded archival tissues including 93 cases of primary NPC and 20 cases of cervical lymphonode metastatic NPC (LMNPC).Results:A total of 15 differential proteins in the 2 cell lines were identified by a proteomic approach,and 3 differential proteins were selectively confirmed.Downregulation of nm23-H1 by siRNA significantly increased the in vitro invasive ability of 6-10B.Significant nm23-H1 downregulation was observed in LMNPC compared with primary NPC.nm23-H1 downregulation in primary NPC was positively correlated with lymphonode and distant metastasis,advanced clinical stage and recurrence.Survival curves showed that patients with nm23-H1 downregulation in primary NPC had a poor prognosis.Multivariate analysis confirmed that nm23-H1 expression level in primary NPC was an independent prognostic indicator.Conclusion:nm23-H1 behaves as a metastasis suppressor in NPC,and nm23-H1 downregulation is a biomarker for poor NPC prognosis.
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<p><b>OBJECTIVE</b>To investigate the expression pattern of hoxd3 gene during early embryogenesis and angiogenesis of wild-type zebrafish.</p><p><b>METHODS</b>Total RNA was extracted from embryos of zebrafish in different development stages by trizol. The cDNA of hoxd3 gene was amplified by RT-PCR. The RT-PCR product was ligated to pCS(2+) vector by T4 DNA ligatase polymerase and sequenced. T3 RNA polymerase in vitro transcription system was used to obtain the probe of digoxin-labeled anti-sense mRNA of hoxd3 gene. The expression pattern of hoxd3 was detected by whole embryo in situ hybridization (WISH) with anti-sense mRNA probe.</p><p><b>RESULTS</b>pCS(2+)-hoxd3 plasmid was successfully constructed, which was used to prepare anti-sense mRNA probe of hoxd3 in vitro. Expression pattern of hoxd3 gene was detected by WISH during zebrafish early embryogenesis and angiogenesis. It was observed that hoxd3 mRNA was expressed at the junction region of midbrain and hindbrain in wild-type zebrafish in embryos at 24 ≊72h postfertilization(hpf).</p><p><b>CONCLUSION</b>hoxd3 gene is mainly expressed in nervous system of wide-type zebrafish embryos.</p>
Subject(s)
Animals , Cloning, Molecular , Gene Expression Regulation, Developmental , Genetic Vectors , Homeodomain Proteins , Genetics , Metabolism , In Situ Hybridization , Plasmids , Genetics , RNA, Messenger , Genetics , Transfection , Zebrafish , Embryology , Genetics , Zebrafish Proteins , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the molecular pathogenesis of protein C (PC) deficiency in a patient with pulmonary embolism and in his family members.</p><p><b>METHODS</b>Anticoagulated blood samples were collected from the proband and his family members to detect PC, PS and AT activities. PC antigen level was measured using ELISA. The genomic DNA was extracted to amplify all the 9 exons and their flanking sequences of PC gene using PCR, and the PCR products were sequenced. The mutated exons identified were amplified and sequenced for the other family members.</p><p><b>RESULTS</b>The proband and his parents and sister were identified as carriers of PC gene mutation, which led to type II PC deficiency. Sequencing of the proband's PC gene showed two heterozygous point mutations in exon 3 (G5540A) and exon 7 (C10230T) to cause compound heterozygous mutations of PC E29K and PC R147W, which were inherited from his father and mother, respectively. His sister was a heterozygote of PC R147W.</p><p><b>CONCLUSION</b>The proband is a compourd heterozygous mutations carrier of PC E29K and PC147W. PC E29K is a novel PC mutation, and PC R147W is a reported PC gene mutation seen in patients with type II hereditary PC deficiency and recurrent thrombosis.</p>
Subject(s)
Adolescent , Humans , Male , Base Sequence , Heterozygote , Molecular Sequence Data , Pedigree , Point Mutation , Protein C , Genetics , Protein C Deficiency , Genetics , Pathology , Pulmonary Embolism , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To analyze the molecular pathogenesis of protein S deficiency in an adolescent case of recurrent deep vein thrombosis (DVT).</p><p><b>METHODS</b>Blood samples from the patient and his family members were collected for detection of the coagulation parameters by one-step clotting method, and the protein S (PS) and protein C activities were measured by a chromogenic assay. Enzyme-linked immunosorbent assay was employed for detecting the levels of free PS antigen. All the exons and exon-intron boundaries of the patients PS gene were amplified using PCR and analyzed by direct sequencing.</p><p><b>RESULTS</b>As carriers of hereditary PS deficiency, both the patient and his father showed a heterozygous C82792T point mutation in the 10th exon of their PS gene which resulted in the substitution of arginine314 by cysteine in the polypeptide chain of PS protein.</p><p><b>CONCLUSION</b>Recurrence of DVT in this patient is the result of hereditary PS deficiency caused by a novel heterozygous missense mutation in the PS gene.</p>
Subject(s)
Adolescent , Humans , Male , Amino Acid Substitution , China , Heterozygote , Mutation, Missense , Pedigree , Point Mutation , Protein S , Genetics , Protein S Deficiency , Genetics , Recurrence , Venous Thrombosis , GeneticsABSTRACT
Minimal residual disease (MRD) is the principal root of relapsed leukemia. Application of specific immunotherapy is effective in eradication of MRD and one of the immunotherapeutic strategies is induction and re-transfusion of leukemia-specific cytotoxic T lymphocytes (CTLs). This study was aimed to investigate the possibility of ex vivo induction and generation of CD8 positive CTLs of umbilical cord blood cell origin and to explore their potential of specific anti-leukemia cytotoxicity so as to evaluate the feasibility of application of cord blood cell derived lymphocytes to specific immunotherapy. Dendritic cells (DCs) were induced and generated ex vivo from cord blood mononuclear cells (MNC) with a cytokine cocktail, and loaded with frozen and thawed U937 leukemia antigen. The matured DCs were used to stimulate T lymphocytes derived from the same cord blood cell sample into CTLs. CD8 positive CTLs were then isolated by magnetic activated cell sorting (MACS). Inverted microscopy, scanning electronic microscopy and flow cytometry were used to detect DCs and methyl thiazolyl tetrazolium (MTT) cytotoxicity method was used to assay the killing activity. The results showed that DCs with typical morphology and mature function were cultured from 10 human cord blood cell samples. The cytotoxicities of CD8 positive CTLs, CD8 negative CTLs and T lymphocytes (TLs) to U937 cells were (66.36 +/- 12.43)%, (34.47 +/- 8.19)% and (15.79 +/- 4.64)% respectively under the same effector target ratio (40:1). Among them, the anti-leukemia cytotoxicity of the CD8 positive group was highest. At effctor target ratio of 40 to 1, the cytotoxicity of CD8 positive CTLs to U937 cells (66.36%) was higher than that to K562 cells (41.97%) (P < 0.05), whereas the cytotoxicity of CD8 negative CTLs to U937 cells was not significantly different from that to K562 cells (P > 0.05). It is concluded that specific CD8 positive CTLs can be generated from cord blood lymphocytes by induction of mature cord blood DCs loaded with U937 leukemia antigen. The cytotoxicity of CD8 positive CTLs against U937 cell is more potent than CD8 negative CTLs, and is strain specific.
Subject(s)
Humans , Antibody Specificity , Antigens, Neoplasm , Allergy and Immunology , CD8-Positive T-Lymphocytes , Allergy and Immunology , Cells, Cultured , Dendritic Cells , Allergy and Immunology , Fetal Blood , Cell Biology , Allergy and Immunology , Immunotherapy , T-Cell Antigen Receptor Specificity , T-Lymphocytes, Cytotoxic , Allergy and Immunology , U937 CellsABSTRACT
<p><b>OBJECTIVE</b>To evaluate clinical outcomes of bracing and analyze related factors that influence curative effects in adolescents with idiopathic scoliosis, and to investigate indications of bracing.</p><p><b>METHODS</b>Seventy-nine patients with AIS who had no history of prior therapy were treated with a brace. Several parameters were consecutive measured and documented during the period of follow-up including Cobb's angles, curve patterns, menarche status, sitting heights, standing heights, Risser sign, apical vertebral rotation, and so on.</p><p><b>RESULTS</b>The average period of followed-up was 30 months (12 months to 60 months). Twenty-one patients (26.6%) presented curve deterioration, 40 patients have no obvious curve change, 18 patients (22.8%) got a curve improvement. There was significantly lower percentage of curve progression and higher percentage of curve improvement in cases with Cobb's angle less than 35 degrees at the first visit (P < 0.05). The percentage of curve progression was significantly greater in the cases with apical vertebral rotation beyond grade III while the percentage of curve improvement was lower (P < 0.05). Curve patterns, Risser sign and other parameters were found to make their effects on the percentage of curve progression and improvement, which, however, was not statistically significant (P > 0.05).</p><p><b>CONCLUSION</b>Bracing can limit or improve mild and moderate curve of idiopathic scoliosis effectively, especially in cases with initial curve magnitude ranging from 20 degrees to 35 degrees . Risser sign is not a reliable parameter for measuring the outcome of bracing treatment for idiopathic scoliosis. Surgery is advised as soon as possible for the cases with initial Cobb's angles greater than 45 degrees and initial apical vertebral rotation beyond grade III early while bracing did not work.</p>
Subject(s)
Adolescent , Child , Female , Humans , Male , Braces , Follow-Up Studies , Scoliosis , General Surgery , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To identify the antithrombin (AT) phenotype and gene mutation of a kindred with hereditary antithrombin deficiency.</p><p><b>METHODS</b>Plasma AT activity and AT antigen level of the propositus and his kindred members were determined with chromogenic substrate method and immunoassay, respectively. All the seven exons and intron-exon boundaries of antithrombin gene were analyzed by PCR and direct sequencing of amplified PCR products from the propositus.</p><p><b>RESULTS</b>The propositus AT antigen level was normal but his AT activity was only 65% of normal value suggesting that he had type II AT deficiency. A heterozygous G13830A mutation in exon 6 resulting in Arg393His missense mutation in his AT polypeptide was identified in the propositus. The same phenotype and gene mutation were found in other 3 kindred members.</p><p><b>CONCLUSION</b>The type II AT deficiency found in this kindred is caused by heterozygous G13830A mutation in AT gene.</p>
Subject(s)
Adult , Humans , Male , Antithrombin III , Genetics , Metabolism , Antithrombin III Deficiency , Genetics , Heterozygote , Mutation , PedigreeABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of GLUT1, p63 and DNA-Pkcs in serous ovarian tumors and their significance.</p><p><b>METHODS</b>GTUL1, p63 and DNA-Pkcs expression at protein level was detected by immunohistochemistry in patients with serous ovarian tumors. Chi-square analysis was used to assess if their expression is associated with clinicopathologic characteristics of the tumors.</p><p><b>RESULTS</b>Cells in the normal ovarian tissues were not stained with GTUL1 and p63 antiserum, but DNA-Pkcs was positively stained. The intensity of GTUT1 and p63 expression was stronger in malignant ovarian serous tumors compared with other subtypes (P < 0.01). There were significant differences of DNA-PKcs among normal ovaries (100.0%), benign (95.0%), borderline (90.0%) and malignant (60.0%) serious ovarian neoplasms (P < 0.01). The level of GLUT-1 expression was correlated with FIGO staging, intraperitoneal implantation, ascites and lymph node metastasis (P < 0.05). p63 expression was associated with clinicopathologic characteristics except ascites (P < 0.05). DNA-PKcs was only correlated with FIGO staging and lymph node metastasis (P < 0.05).</p><p><b>CONCLUSION</b>The results suggest that the abnormal expression of GTUT1, p63 and DNA-Pkcs may perhaps participate in serous ovarian tumor occurrence and development and may be considered as a marker reflecting tumor malignant behavior.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Middle Aged , Young Adult , Cystadenocarcinoma, Serous , Metabolism , Pathology , Cystadenoma, Serous , Metabolism , Pathology , DNA-Activated Protein Kinase , Metabolism , Epithelium , Metabolism , Gene Expression Regulation, Neoplastic , Glucose Transporter Type 1 , Metabolism , Immunohistochemistry , Lymphatic Metastasis , Neoplasm Staging , Nuclear Proteins , Metabolism , Ovarian Neoplasms , Metabolism , Pathology , Ovary , Cell Biology , Trans-Activators , Metabolism , Transcription Factors , Tumor Suppressor Proteins , MetabolismABSTRACT
Based on the understanding of the present situations of bilingual teaching in clinical medical courses together with the analysis of the students' characteristics,the different methods in improving bilingual teaching were explored and the experience was discussed.The problems encountered in bilingual teaching were also discussed.
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To explore the possibility of in vitro induction of cord blood cell-derived lymphocytes into cytotoxic T lymphocytes (CTL) with anti-leukemia specificity, umbilical cord blood (UCB)-derived mononuclear cells were cultured with multiple cytokines to generate dendritic cells (DC) in vitro. Leukemia cells were irradiated with (137)Cs and activated by premature cytokines. The characteristics of maturation of DC were evaluated through morphology examination and flow cytometry. DC pulsed with leukemic antigens were co-cultured with lymphocytes. Cytotoxicity of the CTL to corresponding leukemic cells was measured with lactate dehydrogenase-release assay. The results showed that UCB-derived monocytes could be induced into typical DC in all of the 12 samples. Expression of immunological markers such as CD1a(+), HLA-DR(+), CD86(+), CD83(+) on DC were significantly up-regulated (P < 0.05). DC presenting leukemic antigens generated leukemia-specific CTL with a killing rate of (44.76 +/- 17.42)% at the E:T ratio of 50:1 against AML cells and a killing rate of (8.50 +/- 4.25)% at the E:T ratio of 50:1 against ALL cells. Whereas, these CTL present almost no killing effect on the mononuclear cells collected from the same patients in complete remission phase. It is concluded that (1) it is possible to induce UCB-derived monocytes into mature DC with typical morphology. (2) Cord blood derived mature DC presenting leukemia antigen can generate leukemia-specific CTL with vigorous cytotoxic activity against the same leukemia blasts and low killing activity against bone marrow cells of the same patients in complete remission phase.
Subject(s)
Female , Humans , Male , Cells, Cultured , Coculture Techniques , Cytotoxicity, Immunologic , Allergy and Immunology , Dendritic Cells , Cell Biology , Allergy and Immunology , Fetal Blood , Cell Biology , Allergy and Immunology , K562 Cells , Leukemia , Allergy and Immunology , Pathology , Leukocytes, Mononuclear , Cell Biology , Allergy and Immunology , T-Lymphocytes, Cytotoxic , Allergy and ImmunologyABSTRACT
The aim was to study the morphological, histochemical and immunological characteristics of less-differentiated acute myeloid leukemic cells, and their diagnostic significance. Wright-Giemsa and histochemical staining were used to stain bone marrow smears from 2 case of AML-Mo. Immunological phenotypes were determined with flow cytometry. The results showed that myeloperoxidase stainings of both cases were negative, PAS was positive with fine particles, CD33/CD13 were positive, CD2/CD3/CD10/CD19/CD22 were negative. It is concluded that morphology, histochemistry and immunological phenotype on bone marrow smears are the main diagnostic basis for AML-Mo. The use of multiple monoclonal antibodies for staining may improve the accuracy.