ABSTRACT
Objective To discuss the clinical mechanism of Yanshu injection by observing its function to the tumor cells SGC-7901,HepG2 and BEL-7402 in vitro.Method The kill rate by MTT,flow cytometry were used to detect the change of cell cycle and apoptosis rate,and the expression of bcl-2,CD44V6 and nm23 of HepG2,SGC-7901 and BEL-7402 were observed.Results In vitro,to HepG2,SGC-7901 and BEL-7402,the living rate after using Yanshu injection was 72 %,40 % and 50 %.Compared with the control,the cell quantity in S phase decreased,and the apoptosis rate was higher,the expression of CD44V6 and nm23 changed a lot.Conclusion To the tumor cells,HepG2,SGC-7901 and BEL-7402,Yanshu injection can kill them,arrest them in G2 phase,block the conversion to M phase,accelerate cell apoptosis which has no correlation with the expression of bcl-2,and it still can restrain tumor metastasis.
ABSTRACT
Since 1980s, medical clinical practice turns towards evidence-based medicine model from experimental medical model, traditional TCM diagnosis of tumor difficultly link up with diagnostic methods of modern medicine, and syndrome differentiation typing is difficultly qualified and unified. Clinical studies often lack strict and reasonable design and rigorous manipulation standardization, and do not rapidly adapt changes of modern medicine model. Therefore. in the present paper it is put forward that standardization of clinical diagnosis and assessment of therapeutic effects should be further completed. First establishing and adopting randomized control test of small scale and single center, and using systematical assessing techniques to scientifically evaluate results of relative studies; and scientifically and systematically systematize and strictly re-test and verify rich clinical experiences of famous and older physicians of TCM. In brief, principles of evidence-based medicine should be adopt to guide and standard TCM diagnosis and treatment and scientific studies of malignant tumor.