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Objective Ginsenoside Rh2 can inhibit the proliferation of a variety of malignant tumor cells.However, little research has been done on the sensitivity of Rh2 in human hepatocellular carcinoma HepG2/ADM cells with multidrug resistance(MDR).This study aimed to explore the reversing effects of ginsenoside Rh2 on the MDR of human hepatocellular carcinoma HepG2/ADM cells and its potential mechanism.Methods MTT assay was applied to detect the effect of Rh2(0-250 μg/mL) on the viability of HepG2/ADM cells and screen out the optimum drug-resistant reversal concentration of Rh2.Cells were divided into 3 groups: HepG2/ADM group (without any medicine treatment), ADM group(ADM treatment for 48 h), ADM+40 μg/mL Rh2 group (pretreatment of 40μg/mL Rh2 for 30 min followed by ADM treatment for 48 h).Flow cytometry was applied to detect the effect of Rh2 on the fluorescence intensity of cellular Rh-123.RT-PCR was used to measure the expression of MDR1 gene.Western blot was used to detect the protein levels of P-gp, Bax, Bcl-2 and cleaved caspase-3.Results 40 μg/mL ginsenoside Rh2 significantly reversed the MDR of HepG2/ADM cells by a 2.55-to-3.70-fold increase in sensitivity.Furthermore, compared with ADM group, the efflux of Rh-123 in HepG2/ADM cells were remarkably inhibited by Rh2 in ADM+40 μg/mL Rh2 group (65.83±1.78 vs 78.21±1.26, P<0.01), along with the down-regulated expressions of MDR1 (0.48±0.02 vs 0.86±0.05, P<0.05), P-gp(0.97±0.04 vs 1.91±0.03,P<0.01), Bcl-2(1.25±0.05 vs 1.86±0.03, P<0.05) and the up-regulated protein level of Bax (1.76±0.04 vs 1.25±0.02,P<0.05) and cleved caspase-3(0.42±0.04 vs 38.26±5.45,P<0.05).Conclusion Ginsenoside Rh2 can effectively reverse the MDR of HepG2/ADM cells, and the potential mechanism is related to the decreased expressions of MDR1 and P-gp, the increasing drug concentration inside the cells and the Bax/Bcl-2 signaling pathway.
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Objective To investigate the clinical characteristics and prognostic factors of non-Hodgkin's lymphoma.Methods Sixty-one cases of children with non-Hodgkin's lymphoma in the Central Hospital of Nanyang City from January 2004 to December 2013 were retrospectively analyzed.The overall survival rate and survival rate without recurrence were analyzed by using Kaplan-Meier.And the prognostic factors were analyzed.Results Tumor type:Burkitt's lymphoma 23 cases(37.7％),diffuse large B-cell lymphoma 7 cases(11.5％),B lymphoblastic lymphoma 5 cases(8.2％),B-cell lymphoma 2 cases(3.3％),gradient large T-cell lymphoma 14 cases(23.0％),T lymphoid lymphoma 8 cases(13.1％),and cutaneous T-cell lymphoma 2 cases(3.3％).Stages:stage Ⅰ 3 cases(4.9％),stage Ⅱ 8 cases(13.1％),stage Ⅲ 32 cases(52.4％),and stage Ⅳ 18 cases(29.5％).The 3-year overall survival rate of 61 children was 75.4％(46/61 cases),and 3-year survival rate without recurrence was 68.9％(42/61 cases).Univariate analysis showed that tumor stage(x2=5.158,P<0.05),tumor size(x2=9.173,P<0.05),bone marrow invasion(x2=4.565,P<0.05),spleen invasion(x2=5.306,P<0.05),and central nervous system invasion(x2=5.220,P<0.05)could influence the prognosis of children.Conclusions The prognosis is different for non-Hodgkin's lymphoma children with different pathology,and the tumor stage,tumor size,bone marrow invasion,spleen invasion and central nervous system invasion can significantly influence the prognosis.Children should be treated on the basis of comprehensive analysis of pathology,pathological stage,and prognostic factors.
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BACKGROUND:Dihydroartmisinin can promote apoptosis of glioma cels GL261, but its effect on glioma stem cels is stil unknown. OBJECTIVE:To investigate the preliminary mechanism that dihydroartemisinin inhibits migration and invasion of glioma stem cels. METHODS: Glioma stem cels were isolated from mouse malignant glioma cel lines GL261. Immunofluorescence analysis was conducted to identify the characteristics of glioma stem cels. Migration and invasion abilities of glioma stem cels were analyzed by Transwel assay. The mRNA expressions of Tol-like receptor 2, matrix metaloproteinase-2 and matrix metaloproteinase-9 were examined by real-time fluorescence quantitative PCR. RESULTS AND CONCLUSION:The characteristics of glioma stem cels were identified by CD133 and Nestin staining. The migration and invasion of glioma stem cels were attenuated by dihydroartemisinin dose-dependently. Moreover, the mRNA expression of Tol-like receptor 2, matrix metaloproteinase-2 and matrix metaloproteinase-9 was also decreased by dihydroartemisinin in a dose dependent manner. These results suggest that dihydroartemisinin inhibits the migration and invasion of glioma stem cels probably through attenuation of Tol-like receptor signaling pathway.
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Objective To investigate diagnostic value of ectopic pregnancy by transvaginal sonography (TVCDS) combining with detection of blood β-human chorionie gonadotropin(β-HCG) and progesterone(P) level. Methods 96 ectopic pregnancy patients were exahined by TVCDS,and detected β-HCG and P in the same day or next day,every two days or several days reexamination of β-HCG and P were given to the hospitalization patients. Re-suits 96 ectopic pregnancy patients including massive type 76(79.1%) ,diameter (47.6±18.4) mm;embryo sac type 20(20.9%) ,showed embryo sac structure in ectopic pregnancy area,saw pulsation in saccus vitellinus and fetus vascular color,diameter (12.86±6.85)mm. 94(97.9%) displayed Doppler signals in ectopic pregnancy area, and 89(92.7%)cases were detected low resistivity nutrient artery flow spectrum by PW, the PI average value was (0.46±0.03).96 ectopic pregnancy patients' blood β-HCG and P level were significant low(P<0.01). Conclusion TVCDS combining with detection of β-HCG and P can raise early diagnosis level of ectopie pregnancy.
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@#Objective To explore the effect of rehabilitation guidance combined with family training on motor function and activities of daily living of subacute stroke.Methods 89 patients with subacute stroke were divided randomly into two groups,control group(43 cases)and rehabilitation group(46),receiving routine neurological medication.In addition,rehabilitation group received rehabilitation guidance and training in the meantime.Results After 60 days of rehabilitation training,the scores of Fugl-Meyer Motor Function Assessment(FMA)and Activities of Daily Living improved significantly(P<0.01;P<0.001).Conclusion Rehabilitation guidance combined with family training is effective to improve motor function and activities of daily living of subacute stroke.
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@#ObjectiveTo study the effect of responded hyperglycemia on the prognosis of acute stroke.Methods116 cases with acute stroke were divided into responded hyperglycemia group and normoglycemia group.The complications and activity of daily living(ADL) were compared in these two groups.ResultsResponded hyperglycemia severely influenced the recovery of patients as well as diabetes mellitus did.The incidence of infective complications in responded hyperglycemia group was higher than that of normoglycemia group.ConclusionsResponded hyperglycemia can make the prognosis of acute stroke bad.