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Objective: To understand the antimicrobial resistance and genome characteristics of Campylobacter isolates recovered from retailed poultry meat samples in 20 provinces in China in 2020. Methods: In 2020, 265 Campylobacter strains including 244 Campylobacter jejuni and 21 Campylobacter coli collected from retailed poultry meat samples in China were tested for antimicrobial resistance to 9 antimicrobial compounds by using the agar dilution method. Forty-two selected isolates were sent for whole genome sequencing and 38 high-quality genomes were analyzed for their antimicrobial resistance genes, virulence genes, sequence types and genetic diversity. Results: The resistance rates of Campylobacter isolates from poultry meats to tetracycline, nalidixic acid and ciprofloxacin were the highest (84%-100%), with 53.2% of the isolates showing multidrug resistance in this study. The resistance rates of C. coli to erythromycin, azithromycin, telithromycin, gentamicin and clindamycin were significantly higher than those of C. jejuni (P<0.05). The resistance genes conferring resistance to β-lactams (100%, 38/38), quinolones (94.7%, 36/38), tetracycline (81.6%, 31/38) and aminoglycosides (50%, 19/38) were the most frequently detected among 38 Campylobacter genomes. C. jejuni carried more virulence genes than C. coli. In total, 19 and 17 sequence types (ST) were obtained from 20 sequenced C. jejuni and 18 C. coli isolates, respectively, including 5 novel STs. The isolates showed a high genetic diversity based on their sequence types. Conclusion: The phenomenon of antimicrobial resistance in Campylobacter from poultry meat sources in China is relatively serious, and resistance and virulence genes are widely distributed in Campylobacter. There is genetic diversity in Campylobacter.
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Humans , Animals , Anti-Bacterial Agents/pharmacology , Campylobacter/genetics , Poultry , Drug Resistance, Bacterial/genetics , Genomics , China , TetracyclineABSTRACT
Objective: To understand the antimicrobial resistance and genome characteristics of Campylobacter isolates recovered from retailed poultry meat samples in 20 provinces in China in 2020. Methods: In 2020, 265 Campylobacter strains including 244 Campylobacter jejuni and 21 Campylobacter coli collected from retailed poultry meat samples in China were tested for antimicrobial resistance to 9 antimicrobial compounds by using the agar dilution method. Forty-two selected isolates were sent for whole genome sequencing and 38 high-quality genomes were analyzed for their antimicrobial resistance genes, virulence genes, sequence types and genetic diversity. Results: The resistance rates of Campylobacter isolates from poultry meats to tetracycline, nalidixic acid and ciprofloxacin were the highest (84%-100%), with 53.2% of the isolates showing multidrug resistance in this study. The resistance rates of C. coli to erythromycin, azithromycin, telithromycin, gentamicin and clindamycin were significantly higher than those of C. jejuni (P<0.05). The resistance genes conferring resistance to β-lactams (100%, 38/38), quinolones (94.7%, 36/38), tetracycline (81.6%, 31/38) and aminoglycosides (50%, 19/38) were the most frequently detected among 38 Campylobacter genomes. C. jejuni carried more virulence genes than C. coli. In total, 19 and 17 sequence types (ST) were obtained from 20 sequenced C. jejuni and 18 C. coli isolates, respectively, including 5 novel STs. The isolates showed a high genetic diversity based on their sequence types. Conclusion: The phenomenon of antimicrobial resistance in Campylobacter from poultry meat sources in China is relatively serious, and resistance and virulence genes are widely distributed in Campylobacter. There is genetic diversity in Campylobacter.
Subject(s)
Humans , Animals , Anti-Bacterial Agents/pharmacology , Campylobacter/genetics , Poultry , Drug Resistance, Bacterial/genetics , Genomics , China , TetracyclineABSTRACT
ObjectiveTo investigate the long-term safety of triptolide ferulic acid ethosome gel in percutaneous administration. MethodWe mixed triptolide with ferulic acid to make liposomes gel in different doses and then administrated the gel to SD rats of both sexes with intact skin and damaged skin for 12 weeks. The daily dosages calculated based on triptolide for the low-, middle-, and high-dose groups were 63.75, 127.50, 255.00 μg·kg-1, respectively. The body weight of each rat was measured weekly. The rats were sacrificed in the last week for the determination of serum biochemical parameters and organ indexes as well as the observation of histopathology. The toxicity was assessed based on the body weight and all the parameters and indexes. ResultAfter long-term administration, the body weight and serum biochemical parameters did not show significant difference between the gel-treated groups and the blank group with intact skin, which indicated that the percutaneous administration of triptolide and ferulic acid ethosomes gel was relatively safe. However, the rats in the high-dose group showed sparse hair and were easy to die in the case of unhairing with chloral hydrate at the late stage of the study. Comprared with the female rats with intact skin in the blank control group, the female rats with damaged skin in the middle-dose group showed decreased heart index (P<0.05), which indicated certain cardiotoxicity. Moreover, damage appeared in skin and lung, which may be influeneced by dosage, sex, and skin state. ConclusionFerulic acid in combination with triptolide is relatively safe for percutaneous administration, whereas there are some risks of skin and lung damage in the case of long-term administration. Individualized administration scheme should be developed according to liver and kidney function and skin conditons to ensure the safety of clinical medication.
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Objective To determine the relationship between air pollutants [SO
Subject(s)
Female , Humans , Male , Acne Vulgaris , Air Pollution/adverse effects , China/epidemiology , Environmental Pollutants , Gases , OutpatientsABSTRACT
Objective To investigate the safety and efficacy of wide local excision surgery combined with 5-aminolaevulinic acid(ALA)-photodynamic therapy(PDT)in treating Paget's disease of the scrotum in elderly people.Methods Patients with an average age of 68.4 ± 4.7 years undergoing wide local excision surgery combined with ALA-PDT for Paget's disease of the scrotum from June 2014 to February 2018 were followed up.All patients underwent wide local excision surgical treatment first and were then enrolled in ALA-PDT study after the diagnosis of Paget's disease of the scrotum was confirmed.Four cases were eliminated as a result of two patients refusing photodynamictherapy for various reasons and two patients lost during follow-up after ALA-PDT.A total of 16 patients were included in the study,of whom 6 cases were in Ray stage A1,7 cases in stage A2 and 3 cases in stage B.Patients underwent 3 courses of ALA-PDT after operation.Then the efficacy,shortand medium-term complications were followed up.Results The duration of disease among the 16 patients ranged from 4 to 76 months before diagnosis,with an average of 35.7 months.Surgery was performed immediately after diagnosis.Ten patients underwent resection and suture and 6 patients were treated with skin flap transfers.Of the patients treated with surgery,3 patients received suspicious lymph node dissection and 1 patient underwent reoperation due to skin flap necrosis.Patients were followed up for 3 months to 3 years and 6 months after ALA-PDT.Recurrence and distant metastasis occurred in 2 cases,with 1 case of brain metastasis and 1 case of systemic metastasis,and the overall recurrence rate was 12.5%.During the follow-up,there were no other serious complications except for 1 case(6.3 %)with lower limb movement disorders.Conclusions Wide local excision surgery combined ALA-PDT has good clinical outcomes,low recurrence rates and few complications for the treatment of Paget's disease of the scrotum in elderly people.
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Objective: To study the spatial-temporal dynamical features of hand-foot-mouth disease (HFMD) in Shaanxi Province, so as to provide evidence for the development of relative prevention and control programs on HFMD. Methods: Surveillance data of HFMD was collected from the China Information System for Diseases Control and Prevention from 2009 to 2013. Related data on population and gross domestic product (GDP) was obtained from Shaanxi Statistical Yearbook. Statistical tools as R3.4.1, ArcGIS 10.2 and SaTScan 9.2 software were used to describe the spatial-temporal distribution of the disease. Power-law method on spatial-temporal-multicomponent model was used to analyze the spatial-temporal evolution of the HFMD epidemics in Shaanxi Province. Results: A total of 229 904 cases of HFMD were reported in Shaanxi Province from 2009 to 2013, with an average annual incidence as 122.50 per 100 000. Obvious seasonal characteristics were noticed, with 71.71% of the total cases identified between April and July. Counties with high incidence were mainly distributed in the mid-and east parts of Guanzhong area. Through temporal and spatial scan statistics, we identified that ClassⅠ clustering area was fixed to the central and southeast regions of Shaanxi province which were around Xi'an City between 2009 and 2013, with the relative risk (RR) as 2.24, ranging from 2.18 to 3.08. Results from Power-law analysis showed that the continuous follow-up impact from the previous HFMD epidemics appeared strong in Tongguan, Pucheng districts of Weinan City and Weiyang district of Xi'an, with autoregressive components as 1.14, 0.97 and 0.89, respectively. The risk of HFMD seemed high in Huayin city, Changan and Yanta districts and with the endemic components as 5.08, 4.12 and 4.08, respectively. Impact of the epidemics on nearby districts was largely seen in Lianhu district of Xi'an, Wugong district of Xianyang and Gaoling district of Xi'an with epidemic components as 2.12, 2.08 and 1.77, respectively. The etiological constituents of HFMD were mainly Enterovirus 71 between 2009 and 2012, while HFMD was mainly caused by other entero-viruses, in 2013. Conclusion: Significant spatial-temporal heterogeneity of HFMD was seen in Shaanxi province, which called for specific strategies to be developed in the highly endemic areas.
Subject(s)
Humans , China/epidemiology , Cities , Cluster Analysis , Hand, Foot and Mouth Disease/epidemiology , Incidence , Public Health Surveillance , Spatio-Temporal AnalysisABSTRACT
Objective To study the spatial-temporal dynamical features of hand-foot-mouth disease (HFMD) in Shaanxi Province,so as to provide evidence for the development of relative prevention and control programs on HFMD.Methods Surveillance data of HFMD was collected from the China Information System for Diseases Control and Prevention from 2009 to 2013.Related data on population and gross domestic product (GDP) was obtained from Shaanxi Statistical Yearbook.Statistical tools as R3.4.1,ArcGIS 10.2 and SaTScan 9.2 software were used to describe the spatialtemporal distribution of the disease.Power-law method on spatial-temporal-multicomponent model was used to analyze the spatial-temporal evolution of the HFMD epidemics in Shaanxi Province.Results A total of 229 904 cases of HFMD were reported in Shaanxi Province from 2009 to 2013,with an average annual incidence as 122.50 per 100 000.Obvious seasonal characteristics were noticed,with 71.71% of the total cases identified between April and July.Counties with high incidence were mainly distributed in the mid-and east parts of Guanzhong area.Through temporal and spatial scan statistics,we identified that Class Ⅰ clustering area was fixed to the central and southeast regions of Shaanxi province which were around Xi'an City between 2009 and 2013,with the relative risk (RR)as 2.24,ranging from 2.18 to 3.08.Results from Power-law analysis showed that the continuous follow-up impact from the previous HFMD epidemics appeared strong in Tongguan,Pucheng districts of Weinan City and Weiyang district of Xi'an,with autoregressive components as 1.14,0.97 and 0.89,respectively.The risk of HFMD seemed high in Huayin city,Changan and Yanta districts and with the endemic components as 5.08,4.12 and 4.08,respectively.Impact of the epidemics on nearby districts was largely seen in Lianhu district of Xi'an,Wugong district of Xianyang and Gaoling district of Xi'an with epidemic components as 2.12,2.08 and 1.77,respectively.The etiological constituents of HFMD were mainly Enterovirus 71 between 2009 and 2012,while HFMD was mainly caused by other entero-viruses,in 2013.Conclusion Significant spatial-temporal heterogeneity of HFMD was seen in Shaanxi province,which called for specific strategies to be developed in the highly endemic areas.
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The chemical constituents of the fruits of Chaenomeles sinensis (Thouin) Koehne were investigated using chromatographic methods, including Diaion HP-20, Toyopearl HW-40, MCI Gel CHP-20, ODS, Silica gel chromatography and semi-preparative-HPLC. Three compounds were isolated and their structures were elucidated with spectral data and physicochemical properties, which were identified as chaenomeles alkaloid A (1), ginsenine (2) and 1,2,3,4-tetrahydro-1-methyl-β-carboline-3-car-boxylic acid (3). Among those, compound 1 is a new alkaloid, compound 2 and 3 were isolated from this plant for the first time. To investigate the protective effect of compounds 1-3 on Rat adrenal pheochromocytoma (PC-12) injury induced by the β-amyloid protein (Aβ25-35). The results show that compounds 2 and 3 have a significant protective effect on the PC12 cells exposed to Aβ25-35.
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Objective@#To analyses the antimicrobial resistance and molecular characterization of 21 MRSA isolates cultured from retail foods from different provinces in China, and evaluate the molecular typing methods.@*Methods@#Twenty-one MRSA isolates were obtained from national foodborne pathogen surveillance network in 2012 (Chinese salad, n=3; milk, n=1; cake, n=2; rice, n=1; cold noodle, n=1; spiced beef, n=1; dumpling, n=1; packed meal, n=1; salad, n=1; raw pork, n=9). The antimicrobial resistance of 21 strains to 12 antimicrobial agents was tested by broth dilution method. Polymerase chain reaction (PCR) and DNA sequencing were performed to obtain the genetic types of MLST (ST) and spa typing. The clonal complex (CC) was assigned by eBURST soft and the MLVA type (MT) and MLVA complex (MC) were identified via the database of the MLVA website (http://www.mlva.net). SmaI pulsed-field gel electrophoresis (SmaⅠ-PFGE) was also carried out to obtain the PFGE patterns of 21 strains. The genetic diversity and discriminatory power of typing were calculated by the Simpson's index of diversity (diversity index, DI) to find out the best genotyping method for MRSA.@*Results@#All MRSA isolates showed multi-drug resistance(MDR), and were resistant to oxacillin, benzylpenicillin, clindamycin and erythromycin, and 71.4% (15/21), 47.6% (10/21), 42.9% (9/21) and 28.6% (6/21) of the MRSA isolates were resistant to tetracycline, ciprofloxacin, trimethoprim/sulfamethoxazole and gentamicin, respectively. Moreover, one strain was found to be resistant to all three antimicrobials of levofloxacin, moxifloxacin and rifampicin. Great diversity was found in these food-associated MRSA (6 STs, 7 spa types, and 9 MTs). PFGE patterns were more diverse than those of other three molecular typing methods (19 pulse types). The index of diversity (DI) of PFGE, MLVA, spa typing and MLST was 0.99, 0.80, 0.73, and 0.61, respectively. Among the MRSA isolates, CC9-ST9-t899-MT929-MC2236 (PFGE Cluster Ⅴ) was the most prevalent clone, which were all cultured from raw pork (9 isolates). Besides, two MRSA were identified as CC59-ST338-t437-MT621-MC621 (PFGE Cluster Ⅳ). Different clone had their own resistance spectrum profiles.@*Conclusion@#The food-borne MRSA isolates were all MDR in this study. Different clones had their own resistance spectrum profiles. MLVA represented a promising tool for molecular epidemiology tracing of MRSA in foodborne disease events.
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Objective@#To obtain the serotype diversity and antimicrobial resistance of Salmonella isolates recovered from retail chicken carcasses for sale in six regions of China.@*Methods@#From August 2010 to March 2012, each month 20 retail chicken carcasses including freshly slaughtered, chilled and frozen samples were collected from supermarkets and farmer's markets in 7 monitoring sites in Beijing, Jilin province, Inner Mongolia Autonomous, Shanxi province, Jiangsu province and Guangdong province, respectively. Samples were routinely collected for 12 months for each site. 1 680 chicken carcasses were collected in total and 2 629 Salmonella strains were isolated by PCR and biochemical method. Luminex xMAP method and classical slide agglutination method were carried out to determine isolates' serotypes. Minimal inhibitory concentrations (MICs) of 10 classes of antimicrobials including 14 agents were determined using broth micro-dilution method. Mocular methods were used to determine antimicrobial resistance genes of CIP-CTX-CT co-resistant isolates.@*Results@#In all, 2 629 Salmonella isolates, there were 17 seorgroups and 58 serotypes, B and D1 were the dominant serogroups with rates of 34.7% (n=913) and 31.0% (n=815), Enteritidis (30.8%, n=810), Indiana (17.6%, n=463), Infantis (10.6%, n=278) were the top three serovars. We found 224 CIP-CTX co-resistant S. Indiana containing 3 colistin resistant strains, one of them carrying mcr-1 gene and being ESBLs positive, which demonstrated a nine multi drug resistance against 11 antimicrobials tested.@*Conclusion@#These data began to describe the complicated serovar diversity and heavy antimicrobial resistance of Salmonella isolates recovered from retail chicken carcasses in six regions of China. The findings highlight the emergence of ciprofloxacin and cefotaxime co-resistant S. Indiana and also a mcr-1 positive S. Indiana with heavy multi drug resistance.
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Campylobacter is a major cause of food-borne gastroenteritis worldwide. While mortality is low when people was infected with Campylobacter, morbidity imparted by post-infectious sequelae such as Guillain-Barré syndrome and irritable bowel syndrome is significantly noteworthy. Although fluoroquinolones and macrolides were the first line drug for the treatment of Campylobacter infections, there is a tough challenge in clinical treatment with high antimicrobial resistant rate and multi antimicrobial resistance arise. Based on the latest literature acquired in this work, we have chosen five classes of antibiotics always used in clinical, and discussed antibiotic resistance mechanisms and transmission of Campylobacter, in order to provide proper therapy both in the veterinary and human populations, and support basis data for the development of new drugs.
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Objective To systematically review and evaluate the perioperative indicators and surgical curative effect of 980 nm diode laser vaporization of prostate and transurethral resesction of prostate (TURP) in treating benign prostatic hyperplasia (BPH). Methods Retrieved published comparative studies 980 nm diode laser vaporization of prostate versus transurethral resesction of prostate in treating benign prostatic hyperplasia, and pooled the data from eligible studies. The statistical analysis was performed using Revman 5.3 software. Results Six trials including 839 patients were eligible to the criteria (450 in 980 nm diode laser group and 389 in TURP group). The baseline of patients characteristics were comparable in all the studies. Meta analysis showed that: the operative time was not significantly different between the 980 nm diode laser group and TURP group [SMD = 0.11, 95 ~ CI (-0.52,0.74), P > 0.05]; Compared with TURP group, 980 nm diode laser group has shorter hospital stays [SMD = -1.95, 95%CI (-3.42, -0.48), P 0.05], QOL [SMD = 0.00, 95%CI (-0.57, 0.57), P > 0.05] and Qmax [SMD = 0.06, 95%CI (-0.26, 0.37), P > 0.05]. Conclusion 980 nm diode laser vaporization of prostate is safe and effective in treating benign prostatic hyperplasia, and compared with TURP, it has advantages in shorter hospital stays and shorter catheterization time.
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<p><b>OBJECTIVE</b>To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food.</p><p><b>METHODS</b>Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing.</p><p><b>RESULTS</b>All isolated MRSA exhibited multi-drug resistance (MDR). Greater diversity was found in food-associated MRSA (7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA (3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates (40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone (96.4%), and CC9-ST9-t437-MC621 (20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig- and food-associated isolates. Of note, some community- and hospital-associated MRSA strains (t030, t172, t1244, and t4549) were also identified as food-associated isolates.</p><p><b>CONCLUSION</b>CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.</p>
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Animals , Humans , Anti-Bacterial Agents , Pharmacology , China , Food Microbiology , Methicillin , Pharmacology , Methicillin Resistance , Methicillin-Resistant Staphylococcus aureus , Genetics , Nose , Microbiology , Swine , MicrobiologyABSTRACT
Objective To study the application value of serum follicular statin 1(FSTL-1) in the diagnosis of chronic heart fail-ure(CHF) .Methods A total of 60 CHF children treated from January 2013 to January 2016 in hospital were selected as observa-tion group .According to New York Heart Association (HYHA) ,the subjects were divided into Type Ⅱ group ,Type Ⅲ group and Type Ⅳ group ,20 cases in each group .In addition ,60 healthy children were selected as control group .FSTL-1 ,NT-proBNP and LVMI were detected and analyzed .Results FSTL-1 ,NT-proBNP and LVMI in Type Ⅱ group ,Type Ⅲ group and Type Ⅳ group were higher than those of control group(P< 0 .05) .As the grade of cardiac function increasing ,FSTL-1 ,NT-proBNP and LVMI were higher(P<0 .05) .FSTL-1 had positive correlations with NT-proBNP and LVMI .The positive rate of FSTL-1 for diagnosis CHF was 96 .67% ,which had no significant difference with gold standard (P>0 .05) .Conclusion With the progress of illness ,the level of FSTL-1 increases ,which is importance in the diagnosis and monitoring of CHF .It is worthy of further application and pro-motion in clinical practices .
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Objective To study the application value of serum follicular statin 1(FSTL-1) in the diagnosis of chronic heart fail-ure(CHF) .Methods A total of 60 CHF children treated from January 2013 to January 2016 in hospital were selected as observa-tion group .According to New York Heart Association (HYHA) ,the subjects were divided into Type Ⅱ group ,Type Ⅲ group and Type Ⅳ group ,20 cases in each group .In addition ,60 healthy children were selected as control group .FSTL-1 ,NT-proBNP and LVMI were detected and analyzed .Results FSTL-1 ,NT-proBNP and LVMI in Type Ⅱ group ,Type Ⅲ group and Type Ⅳ group were higher than those of control group(P< 0 .05) .As the grade of cardiac function increasing ,FSTL-1 ,NT-proBNP and LVMI were higher(P<0 .05) .FSTL-1 had positive correlations with NT-proBNP and LVMI .The positive rate of FSTL-1 for diagnosis CHF was 96 .67% ,which had no significant difference with gold standard (P>0 .05) .Conclusion With the progress of illness ,the level of FSTL-1 increases ,which is importance in the diagnosis and monitoring of CHF .It is worthy of further application and pro-motion in clinical practices .
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Objective To evaluate the clinical efficacy and safety of retroperitoneal laparoscopy and open surgical treatment of simple renal cyst by Meta analysis.Methods Computer retrieval of MED-LINE,Cochrane Library,EMBASE,CNKI,Wanfang and VIP databases,according to the inclusion and exclusion criteria collected and screened out a randomized controlled studies on the treatment of simple renal cysts,published from January 1,2000 to August 31,2015.Two independent authors screened out randomized clinical trials (RCTs),extracted data,assessed quality of data,and then carried out Meta analysis by Revman 5.0 software with related data.Results Twenty one studies met the inclusion criteria with a total of 2 296 patients,including 1 162 patients of retroperitoneal laparoscopy and 1 134 cases of open operation group.Compared to the open operation group,retroperitoneal laparoscopy showed shorter operation time,less bleeding,shorter hospitalization time,and earlier postoperative drainage tube extraction time.No significant difference was found in the recurrence rate of the cyst for two groups.Conclusions The clinical efficacy,safety,and complication of retroperitoneal laparoscopy in the treatment of simple renal cyst is better than the open operation.
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OBJECTIVE@#To detect the levels of neutrophil gelatinase-associated lipocalin (NGAL), cystatin C (Cys-C ) in blood and the level of kidney injury molecule 1 (KIM-1) in urine in elderly patients with renal calculi at diff erent times, and to explore the eff ect of percutaneous nephrostolithotomy (PCNL) combined with flexible ureteroscopy (FU) on early postoperative renal function.@*METHODS@#A total of 46 patients with renal calculi were selected, and their blood or urine specimens were collected respectively at preoperative and postoperative 2, 12, 24, 48, and 72 h. The concentrations of NGAL, Cys-C, KIM-1 were detected.@*RESULTS@#The levels of NGAL and Cys-C began to increase respectively at postoperative 2 and 12 h, and reached peak at postoperative 12 to 24 h. There was significant difference in the levels of NGAL and Cys-C between the postoperative 12 and 2 h or between postoperative 48 and 24 h (all P<0.05). The levels of NGAL and Cys-C began to decline and eventually returned to preoperative levels respectively at postoperative 48 and postoperative 72 h. The KIM-1 began to increase at postoperative 2 h and peaked at postoperative 24 h, which was significant difference between the postoperative 24 and 12 h or postoperative 48 and 24 h (both P<0.05). The level of KIM-1 began to decline and eventually returned to preoperative levels at postoperative 48 h.@*CONCLUSION@#After the combined treatment of percutaneous nephrostolithotomy with flexible ureteroscopy, the concentrations of NGAL, Cys-C and KIM-1 are significantly increased, suggesting injuries on renal function. The time of renal tubular injury and recovery is earlier than that of renal glomerulus.
Subject(s)
Aged , Humans , Acute-Phase Proteins , Urine , Cystatin C , Blood , Urine , Hepatitis A Virus Cellular Receptor 1 , Kidney , Kidney Calculi , General Surgery , Lipocalin-2 , Lipocalins , Blood , Urine , Membrane Glycoproteins , Blood , Urine , Nephrostomy, Percutaneous , Postoperative Period , Proto-Oncogene Proteins , Blood , Urine , Receptors, Virus , Blood , UreteroscopyABSTRACT
<p><b>OBJECTIVE</b>To determine Campylobacter contamination level and antimicrobial resistance patterns from chicken carcasses in supermarkets and farmer's markets of 9 districts in Beijing.</p><p><b>METHODS</b>From August 2012 to July 2013, whole chicken carcasses (n = 240) were collected from 27 supermarkets and 18 farmer's markets of nine districts in Beijing. The level of Campylobacter contamination was enumerated by plate counting method using the modified Karmali and modified Preston agar. Presumptive Campylobacter isolates were identified and characterized by gram stain, agglumination test and a multiplex PCR method. The level of Campylobacter contamination was calculated following the USDA/FSIS Campylobacter enumeration method. Selected 151 Campylobacter isolates were further characterized by minimal inhibitory concentrations(MICs) of eight antimicrobials.</p><p><b>RESULTS</b>A total of 26.3% (63/240) of the retail whole chicken carcasses were contaminated by Campylobacter and 151 Campylobacter isolates were recovered, including 85 Campylobacter jejuni isolates and 66 Campylobacter coli isolates. The P25, P50, P75 of Campylobacter contamination concentration were 7.5, 45.0 and 350.0 CFU/g, respectively. The antimicrobial resistance rate of C. jejuni and C. coli were as the following: azithromycin(AZI, 13% (11/85), 82% (54/85)), chloramphenicol (CHL, 33% (28/85), 42% (28/85)), ciprofloxacin (CIP, 95% (81/85), 100% (85/85)), doxycycline (DOX, 38% (32/85), 80% (53/85)), erythromycin (ERY, 12% (10/85), 82% (54/85)), gentamicin (GEN, 25% (21/85), 68% (45/85)), tetracycline (TET, 67% (57/85), 73% (62/85)), all isolates were susceptible to meropenem (MEP). The multi-drug resistance ratio of C. jejuni (55% (47/85) )was significantly lower than that (86% (57/66) )of C. coli (χ(2) = 16.70, P < 0.01). Among 151 Campylobacter isolates, 21 antimicrobial resistance patterns were identified, including 20 patterns among C. jejuni isolates and 10 patterns among C.coli isolates. Among C.jejuni isolates, CIP-DOX-TET was dominant (22% (19/85)), followed by CIP-TET (14% (12/85)), CHL-CIP-TET(9% (8/85)) and CHL-CIP-GEN (7% (6/85)). Among C.coli isolates,AZI-CHL-CIP-DOX-ERY-GEN-TET (35% (23/66)) was the dominant, followed by AZI-CIP-DOX-ERY-GEN-TET (21% (14/66) )and AZI-CIP-DOX-ERY-TET(15% (10/66)).</p><p><b>CONCLUSION</b>Our findings showed a high prevalence and concentration of Campylobacter contamination in retail chicken carcasses of nine districts in Beijing, especially the on-site slaughtered chicken from the farmer's markets. The resistance levels of these recovered Campylobacter isolates were serious.</p>
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Animals , Anti-Bacterial Agents , Campylobacter coli , Classification , Campylobacter jejuni , Classification , Chickens , Drug Resistance, Multiple, Bacterial , Food Microbiology , Meat , Microbial Sensitivity TestsABSTRACT
Reliable transport of Campylobacter jejuni isolates is critical to microbial epidemiology research, especially in developing countries without a good temperature control mailing system. Various factors, including oxygen, temperature, transport medium composition, could affect the survival of C. jejuni. In this study, the protective effects of different ingredients in C. jejuni transport media at 4 °C and 25 °C and under aerobic condition were quantitatively evaluated respectively. The results showed that enriched medium, supplementation with 5% blood and being kept at 4 °C could improve the viability of different C. jejuni strains during transport. In addition, supplementation with 25 mmol/L L-fucose in Wang's transport medium could significantly improve the survival of C. jejuni at both 4 °C and 25 °C. To the best of our knowledge, this is the first report to evaluate the protective effect of L-fucose in enriched C. jejuni transport medium which is feasible in developing countries without an effective cold chain mailing system. These data will be good reference for C. jejuni transport medium improvement in future.
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Bacteriological Techniques , Campylobacter jejuni , Culture MediaABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between maternal milk and serum thyroid hormones in patients with thyroid-related diseases.</p><p><b>METHODS</b>Serum and breast milk samples were collected from 56 breastfeeding mothers. Milk and serum free triiodothyronine (FT3), free thyroxine (FT4), triiodothyronine(T3), thyroxine (T4), and thyrotrophin (TSH) were determined, and T3/T4 was calculated. Using the serum thyroid hormones as the independent variables and milk thyroid hormones as the dependent variables, we performed linear regression analysis.</p><p><b>RESULTS</b>The milk FT3, FT4, T3, T4, TSH, and T3/T4 were (2.30 ± 0.82) pg/ml ,(0.45 ± 0.26) ng/dl, (0.35 ± 0.20) ng/ml, (2.96 ± 1.55) Μg/dl, (0.12 ± 0.08) ΜU/ml, and 0.12 ± 0.04, respectively. Milk FT3 (r = 0.778, P = 0.000), T3 (r = 0.603, P = 0.000), T4 (r = 0.485, P = 0.004), and TSH (r = 0.605, P = 0.000) concentrations were positively correlated with those in serum.</p><p><b>CONCLUSION</b>Thyroid hormones are present in human milk and are positively correlated with those in serum.</p>