ABSTRACT
Objective To investigate the curative effect of pterygium excision combined with mitomycin C on scleral melting and the prevention of pterygium excision. Methods 54 patients(54 eyes)with clinical pterygium excision combined with mitomycin C who were admitted in our hospital from July 2015 to May 2017 were selected as the observation group, and they were not treated with hormones eye drops after surgery continuously. The 54 patients were given non-hormones eye drops, as well as Beifushu eye ointment until complete recovery. 36 patients admitted in our hospital from July 2013 to June 2015 were chosen as the control group and were treated with pterygium excision. The clinical treatment effect between the two groups was compared. Results All patients underwent conservative treatment with drugs after surgery. After treatment, the scleral appearance of the patients was flat and recovered completely. The effective rate of clinical treatment was 96. 30％ in 54 patients of the observation group and 80. 56％ in 36 patients of the control group. The scleral appearance of all patients was flat and completely repaired without any complications. No patient had recurrence in the observation group. And 2 patients had recurrence in the control group. Pterygium excision combined with mitomycin C surgery had less safety and better clinical treatment effect compared with simple pterygium excision, which would be paid more attention to in the application to ensure the safety of patients. Conclusion Pterygium excision combined with mitomycin C can effectively improve the clinical efficacy in clinical practice, but will lead to local tissue burns during the operation. Therefore, the concentration and operating time of mitomycin C in the clinical treatment should be paid attention to, to prevent the occurrence of scleral melting.
ABSTRACT
The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.