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Objective@#To construct the BCMA-CAR using the B-cell maturation antigen (BCMA) specific ligand APRIL as antigen binding region and to validate the effect of BCMA-CAR modified T cells (BCMA-CAR-T) on myeloma cells.@*Methods@#The BCMA-CAR was constructed using the BCMA specific ligand APRIL as antigen binding domain and 4-1BB as the costimulatory domain. The specific cytotoxicity against BCMA+ myeloma cell lines and primary multiple myeloma (MM) cells in vitro were evaluated. In addition, BCMA+ myeloma xenograft mouse model was established to assess the anti-tumor effect of BCMA-CAR-T cell therapy in vivo.@*Results@#BCMA-CAR-T cells could specifically kill BCMA+ myeloma cell lines (For BCMA-CAR-T cells, BCMA+ cells are almost undetectable in the E∶T ratio of 1∶4) and MM patients’ bone marrow mononuclear cells (the proportion of residual cells in BCMA-CAR-T and vector-T groups was 16.0% vs 66.85%, P=0.003) with significant degranulation (CAR-T and vector-T cells cocultured with MM1.S, H929 and U266 had degranulation levels of 33.30% vs 5.62%, 16.97% vs 2.95% and 25.87% vs 2.97%, respectively, P<0.001) and cytokines release (P<0.01) in vitro. In a human BCMA+ myeloma xenograft mouse model, BCMA-CAR-T cells could significantly prolong the survival of mice (The median survival time of mice treated with BCMA-CAR-T and vector-T cells was 87.5 days and 67.5 days, respectively, P<0.001) .@*Conclusion@#The ligand-based BCMA-CAR-T cells could be a promising strategy for BCMA+ multiple myeloma treatment.
ABSTRACT
Objective To discuss the importance of nutritional intervention through monitoring the growth and the nutritional status of children with food allergy after comprehensive nutritional intervention.Methods A total of 158 children with food allergy who were under 2 years old were divided into an intervention group and an control group according to comprehensive nutritional intervention.The intervention group received diet guidance from a dietitian regu larly and took probiotics.If necessary,nutrients (calcium,iron and zinc) could be provided.The control group was given oral guidance by the pediatrician.After 6 months 1 follow-up,growth situation was assessed in terms of height and weight indexes,and nutritional intake condition was assessed in the light of serum biochemical indexes.Results The prevalence of malnutrition in the intervention group(17.6％) was significantly lower than that in the control group (37.0％),and there was a statistical sigmficance (x2 =22.03,P ＜ 0.05);the level of zinc [(88.5 ± 9.6) μmol/L]and iron [(18.6 ± 3.4) mmol/L] in the serum were significantly higher than those [zinc:(68.2 ± 8.5) μmol/L;iron:(12.8 ±2.7) mmol/L] in the control group,and there were statistical significance (zinc:t =12.264,P ＜ 0.01;iron:t =10.762,P ＜ 0.05);the daily intake of calcium[(596.1 ± 110.3) mg] and zinc [(10.7 ± 3.7) mg] in children (＞ 6-24 months) of the intervention group were significantly higher than those [calcium:(412.8 ± 89.3) mg and zinc:(7.3 ± 2.1) mg] in the control group (calcium:t =4.011,P ＜ 0.05,zinc:t =5.367,P ＜ 0.05),and the daily intake of protein [(42.8 ± 9.4) g] in the children (＞ 12-24 months) of the intervention group was significantly higher than that [(33.2 ± 8.2) g] in the control group,and there was a statistical significance (t =3.245,P ＜0.05).Conclusions Children with food allergy have risks of malnutrition,while comprehensive nutritional intervention can obviously decrease the prevalence of malnutrition and deficiency of iron,zinc and calcium,hence normative guidance of scientific feeding of children with food allergy is needed.
ABSTRACT
BACKGROUND: The training task for aircrew of army airmen is very heavy, the evaluation of flight personnel on psychological diathesis is performed to early discover and deal with corresponding questions.OBJECTIVE: To compare the difference of mental health between aircrew and ground crew of army airmen and provide reference data for implementing corresponding norms.DESIGN: Chester sampling investigation and paired design were applied.SETTING: the 71687 Force of Chinese PLA, the 71521 Force of Chinese PLA, Department of Epidemiology, Fourth Military Medical University of Chinese PLAPARTICIPANTS: By chester sampling, 122 aircrew and 122 ground crew of one army airmen in one stationed training troop were randomized in February 2001. The pairs were made at ratio of 1:1 according to age difference less than 3 years and same sex and nationality. The investigated persons were all male, of Han and aged varied from 23 to 52 years.METHODS: Cornell health questionnaire was adopted respectively in investigation of aircrew and ground crew of army airmen. The investigated persons finished and handed in questionnaire independently on the spot in manner of secretion and centralized filling-up. Cornell health questionnaire is composed of 18 sections, including 195 questions, involving 4 aspects,named somatic symptoms, medial and family history, general health and habit and mental symptoms. The total score of Cornell health questionnaire ≥ 30 indicates somatic and mental disturbance and the score of mental section ≥ 10 indicates mental disturbance, both of which are taken as the criteria in comparison of psychological health in receptors of two groups.MAIN OUTCOME MEASURES: Every factor score and total score in somatic and mental sections in Cornell health questionnaire for aircrew and ground crew.RESULTS: The tested results of each 122 cases in either aircrew group or ground crew group entered result analysis. Evaluated results of somatic and mental symptoms in receptors of two groups: the total score was low in Cornell health questionnaire (0-14 scores), of which, the total score in aircrew group was higher than that in ground crew group (81.5% and 59.8%,P ＜ 0.05); the score in mental section was low (0-9 scores), of which, that in aircrew group was higher remarkably than that in ground crew group (99.2% and 91.8%, P ＜ 0.01); the score for the receptors with somatic and mental disturbance (total score≥30, score in mental section ≥ 10) in aircrew group was lower than those in ground crew group (4.1% and 12.3%, P ＜ 0.05); the score for the receptors with mental disturbance (score in mental section≥ 10) in aircrew group was lower remarkably than ground crew group (0.8% and 8.2%, P ＜ 0.01). For somatic section in aircrew group, except the factors of muscle, skeleton and skin, their scores were higher than those in ground crew group (P ＞ 0.05), the scores of the rest factors were all lower than those in ground crew group, of which, the difference was significant in evaluation of factors of eye, ear, nervous system, urinary reproductive system and fatigue in comparison of two groups (P ＜ 0.05). The score of every factor in mental section of aircrew group was lower than those in ground crew group. Except tension factor, in comparison of the rest factors in two groups, the significant difference was present (P ＜ 0.05).CONCLUSION: Overall psychological health state in Cornell questionnaire of aircrew in army airmen was superior to ground crew. Aircrew still presents a part of psychological health questions, mainly in somatic section and inadaptability was the most positive response in mental section, suggesting that aircrew probably presents inadaptable phenomena.