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Objective:To investigate the effect of rhein on aquaporin 4 （AQP4） and brain edema after cerebral ischemia and the role of microglia-mediated inflammation in this process. Method:The modified thread embolization method was selected to establish the cerebral ischemia model of the right middle cerebral artery embolism （MCAO） in rats. The rats were divided into sham operation group， model group， minocycline group， and high， medium and low-dose rhein groups （3.46，1.73，0.865 mg·kg<sup>-1</sup>）. The neurobehavioral function was measured by a modified neurobehavioral score. Wet and dry weight methods were used to measure the changes of water content in brain tissue of rats with cerebral ischemic injury. Western blot was used to detect the expressions of interferon-<italic>γ</italic> （IFN-<italic>γ</italic>） and interleukin-2 （IL-2） in the peripheral ischemic area of rats in each group. Immunofluorescence double labeling method was used to detect the expressions and localization of microglia fine markers Iba-1 and AQP4. Result:Compared with the sham operation group， neurological function score and water content on the side of brain tissue injury of the model group were significantly increased （<italic>P</italic><0.05）. Compared with the model group， the neurological function score and the water content of the brain tissue of each drug group were reduced （<italic>P</italic><0.05， <italic>P</italic><0.01）. Compared with sham operation group， the protein expressions of IFN-<italic>γ</italic> and IL-2 in the model group increased significantly （<italic>P</italic><0.05）. Compared with the model group， the protein expressions of IFN-<italic>γ</italic> and IL-2 in the peripheral area of cerebral ischemia of each drug group were significantly improved （<italic>P</italic><0.05， <italic>P</italic><0.01）. Immunofluorescence double staining results showed that compared with the sham operation group， the model group showed significant increase in the fluorescence expression of AQP4 protein on activated microglia， while each drug group could reduce the fluorescence expression of AQP4 protein on activated microglia， different levels of activated microglia markers Iba-1 and AQP4 were co-localized in the peripheral area of cerebral ischemia in each group. Conclusion:Rhein could reduce the degree of brain edema caused by cerebral ischemic injury， and its mechanism may be related to the inhibition of microglia-mediated neuroinflammation and the down-regulation of AQP4 expression.
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OBJECTIVE@#To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest (EBN) in ovariectomized rats.@*METHODS@#Forty-two female Sprage-Dawley rats were randomized into 7 groups (6 in each group). The ovariectomized (OVX) and OVX + 6%, 3%, and 1.5% EBN and OVX +estrogen groups were given standard rat chow alone, standard rat chow +6%, 3%, and 1.5% EBN, or standard rat chow +estrogen therapy (0.2mg/kg per day), respectively. The sham-operation group was surgically opened without removing the ovaries. The control group did not have any surgical intervention. After 12 weeks of intervention, blood samples were taken for serum estrogen, osteocalcin, and osteoprotegerin, as well as the measurement of magnesium, calcium abd zinc concentrations. While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique, then collected for histology and estrogen receptor (ER) immunohistochemistry.@*RESULTS@#Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain, while estrogen and EBN supplementation attenuated these changes. Ovariectomy also reduced bone ER expression and density, and the production of osteopcalcin and osteorotegerin, which are important pro-osteoplastic hormones that promote bone mineraliztion and density. Conversely, estrogen and EBN increased serum estrogen levels leading to increased bone ER expression, pro-osteoplastic hormone production and bone density (all P<0.05).@*CONCLUSION@#EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.
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Objective Establishment of orthotopic transplantation tumor model of human choriocarcinoma in nude mice. Methods Human choriocarcinoma cell line JAR was cultured and the single cell suspension was subcutaneously injected into five 8-week-old BALB / c nude mice to establish subcutaneous xenograft model. After subcutaneous tumor was formed in nude mice, the tumor tissue was taken under aseptic conditions and cut into 1 mm
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Objective To investigate the expression of semaphorin 3A (Sema3A) and its receptor neuropilin-1 (NRP-1) in gastric cancer and its correlation with microvessel density (MVD), and then to explore the effect of recombinant human Sema3A on angiogenesis of gastric cancer and the associated mechanisms. Methods Forty cases of gastric cancer tissues and its corresponding adjacent normal tissues were used to detecte the expression of Sema3A, NRP-1 and MVD in tissues by immunohistochemistry method . The expression level of Sema3A in serum of gastric cancer patient group and normal control group were measured by Enzyme-linked immuno-sorbent assay (ELISA). Western blotting was used to detect the expression of Sema3A and NRP-1 in five gastric cancer cell lines (MGC-803,HGC-27,MKN-28,SGC-7901,MKN-45) and human gastric mucosal epithelial cell (GES-1). Transwell chamber was used to construct non-contact in vitro co-culture system, in which the effects of different concentrations of recombinant human Sema3A on angiogenesis in gastric cancer were analyzed by tube formation assay preliminarily. The expression levels of vascular endothelial growth factor receptor 2 (VEGFR2) and NRP-1 in co-culture system were detected by Western blotting. Results The expression levels of Sema3A in gastric cancer tissues, cell lines and patient serum were significantly lower than that in the control group(P<0. 05), while the expression of NRP-1 in gastric cancer tissues and MKN-28 cells was significantly increased, and both of them were associated with TNM staging of gastric cancer (P < 0. 05) . In vitro co-culture system, The tube forming abilities of human umbilical vein endothelial cell (HUVEC) were decreased in recombinant human Sema3A treated group, and this phenomenon was concentration dependent. The expression of VEGFR2 protein was down-regulated by recombinant human Sema3A. Conclusion The expression of Sema3A was decreased in gastric cancer tissues, cell lines and patient serum, and negatively correlated with microvessel density. The recombinant human Sema3A could inhibit the angiogenesis of gastric cancer in vitro, which may be related to down-regulation of VEGFR2 protein expression.
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With the standardization and popularization of cervical cancer screening techniques,more and more young patients with cervical cancer are being detected at an early stage,and most patients with cervical cancer at reproductive age have a strong intention to preserve fertility.This article will summarize the operation indication,operation methods,safety,oncologic outcomes and related controversies of fertility preservation surgery in patients with cervical cancer.
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@#AIM: To explore the effects of vitrectomy simultaneously combined with phacoemulsification on visual acuity, intraocular pressure(IOP), central macular thickness(CMT)and postoperative complications in patients with proliferative diabetic retinopathy(PDR).<p>METHODS:Totally 90 PDR patients(98 eyes)admitted to the hospital from February 2015 to February 2017 were divided into two groups according to the principle of voluntariness: group A(vitectomy simultaneously combined with lensectomy, 45 cases, 50 eyes)and group B(vitrectomy simultaneously combined with phacoemulsification, 45 cases, 48 eyes). The follow-up period was 6mo. The visual acuity improvement, IOP before and after surgery, CMT and incidence of postoperative complications were compared between the two groups.<p>RESULTS: The visual acuity improvement after surgery in group B was significantly better than that in group A, and the improving rate of visual acuity was significantly higher than that in group A(<i>P</i><0.05). There was no significant difference in IOP before and after surgery between the two groups(<i>P</i>>0.05). The CMT after surgery was decreased significantly in both groups. CMT in both groups was not statistically different(<i>P</i>>0.05). The incidence rates of postoperative complication ssuch as INV, capsule turbidity, corneal edema, pupil hijacking and hard exudate in group B were significantly lower than those in group A(<i>P</i><0.05).<p>CONCLUSION:Vitrectomy simultaneously combined with phacoemulsification have obvious efficacy on PDR, which promote recovery of postoperative visual acuity, reduce CMT, and it has low risk of postoperative complications.
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@# Objective To investigate the relationship between interleukin 10 (IL-10) -592 (rs1800872) and -819 (rs1800871) promoter genetic polymorphisms and the susceptibility of antituberculosis drug-induced liver injury (ADLI). Methods A case-control study was conducted. Epidemiology survey data and peripheral blood samples were obtained from the patients. Two IL-10 gene polymorphisms (-592 A/C and 819 C/T) were genotyped with PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) in Chinese Han ADLI subjects (n=180) and sex matched by frequency matching in control subjects (n=180). Results No significant differences in genotypes of IL-10 -592 site and IL-10 -819 site between ADLI group and that of the control group were noticed (all P>0.05). The mutant alleles -592 C of IL-10 gene polymorphism was significantly higher in ADLI subjects compared to controls, and in dominant model, the frequency of CC+AC genotype was 1.62 higher among the cases than controls (all P<0.05). Significant difference in allele -819 C/T between the ADLI group and the control group were not found (P=0.190). The polymorphisms at -819 C/T and -592 A/C variants of IL-10 gene were found to be good linkage disequilibrium. The CC haplotype represent genetic risk factor (OR=1.37, 95% CI: 1.02-1.85) and CA haplotype represent genetic protect factor (OR=0.49, 95% CI: 0.34-0.70) for ADLI in the subjects. Conclusions The polymorphisms in IL-10 gene -592 A/C and -819 C/T are associated with ADLI.
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The purpose of this study was to determine the ion release from four commercially available pit-and-fissure sealants [3M Clinpro,3M ConciseTM,BeautiSealant (BS),and GI FX-Ⅱ)].With each brand,18 specimens were prepared.Their fluoride release in de-ionized water was measured by fluoride electrode,while the release of silicate (Si),aluminum (A1),sodium (Na),calcium (Ca),strontium (Sr),and phosphorus (P) was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) on days 1,3,7,14,21,and 28.The result showed that fluoride was not released from 3M ConciseTM.GI FX-Ⅱ displayed the largest fluoride release,which,however,dropped rapidly on day 3.3M Clinpro exhibited less fluoride release than GI FX-Ⅱ and BS did.At any time during the 28-day experimental period,GI FX-Ⅱ released more Na than the other sealants (P＜0.001).BS ranked the second in Na release,and a small amount of Na ions was released from the 3M Clinpro and 3M ConciseTM samples.A1 ions were only detected from BS and GI FX-Ⅱ,but not from the 3M Clinpro and 3M ConciseTM.Additionally GI FX-Ⅱ had the largest Si release among the four brands at any time during the experimental period (P＜0.001).Ca ions were detected from 3M Clinpro and 3M ConciseTM,but not from GI FX-Ⅱ.BS released more Sr than the other sealants at any time during the experimental period (P＜0.001).All the samples released similar amounts of P continuously during these 28 days.In conclusion,based on the type and the amount of ion release,BS is the best pit-and-fissure sealant among the four brands.
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The purpose of this study was to determine the ion release from four commercially available pit-and-fissure sealants [3M Clinpro,3M ConciseTM,BeautiSealant (BS),and GI FX-Ⅱ)].With each brand,18 specimens were prepared.Their fluoride release in de-ionized water was measured by fluoride electrode,while the release of silicate (Si),aluminum (A1),sodium (Na),calcium (Ca),strontium (Sr),and phosphorus (P) was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) on days 1,3,7,14,21,and 28.The result showed that fluoride was not released from 3M ConciseTM.GI FX-Ⅱ displayed the largest fluoride release,which,however,dropped rapidly on day 3.3M Clinpro exhibited less fluoride release than GI FX-Ⅱ and BS did.At any time during the 28-day experimental period,GI FX-Ⅱ released more Na than the other sealants (P＜0.001).BS ranked the second in Na release,and a small amount of Na ions was released from the 3M Clinpro and 3M ConciseTM samples.A1 ions were only detected from BS and GI FX-Ⅱ,but not from the 3M Clinpro and 3M ConciseTM.Additionally GI FX-Ⅱ had the largest Si release among the four brands at any time during the experimental period (P＜0.001).Ca ions were detected from 3M Clinpro and 3M ConciseTM,but not from GI FX-Ⅱ.BS released more Sr than the other sealants at any time during the experimental period (P＜0.001).All the samples released similar amounts of P continuously during these 28 days.In conclusion,based on the type and the amount of ion release,BS is the best pit-and-fissure sealant among the four brands.
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This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days. Co-culture experiments were divided into RAW264.7 cell group, psoralen+RAW264.7 cell group, without psoralen treatment of CD4+T cells+RAW264.7 cell group, psoralen treatment of CD4+T cells+RAW264.7 cell group. After 5 days of co-culture, TRAP staining was used to detect the number of osteoclasts, and after 8 days of co-culture, bone resorption was evaluated by toluidine blue staining. The expressions of RORγt, Foxp3, IL-17, TNF-α, TGF-β and IL-10 in CD4+T cells and osteoclast differentiation-related genes MMP-9, TRAP and Cat-K were detected by Real-time polymerase chain reaction (RT-PCR); ELISA kit was used to detect IL-17, TNF-α, TGF-β and IL-10 and other cytokines levels. Our data confirmed that the psoralen significantly promoted the expression of Foxp3, TGF-β and IL-10 in CD4+T, and inhibited the expression of RORγt, IL-17 and TNF-α in CD4+T, the CD4+T cells without treatment by psoralen can significantly promote RANKL-induced differentiation of RAW264.7 to osteoclasts, and psoralen treatment of CD4+T can significantly inhibit RANKL-induced RAW264.7 osteoclast differentiation and bone resorption. Taken together, psoralen inhibits the differentiation and bone resorption of RAW264.7 into osteoclasts by promoting the development of CD4+ CD25+ Treg/Th17 balance in CD4+T cells to CD4+CD25+T.
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Objective To investigate the relationship between methylation of CpG island in glutathione S-transferase P1(GSTP1) promoter region and injury induced by isoniazid in HL-7702 cells.Methods HL-7702 cells were divided into the control group and three isoniazid groups(200,400,800 mg/L).Colorimetric method was used to detect the activity level of lactate dehydrogenase in the medium of HL-7702 cells;the mRNA expression of GSTP1,DNA methyltransferases 1(DNMT1),DNMT3a and DNMT3b were de?tected by real-time fluorescence quantitative PCR;the protein expression levels of DNMT1,DNMT3a and DNMT3b were detected by enzyme-linked immunosorbent assay;the methylation of the CpG island in the GSTP1 promoter region was determined by the bisulfite sequencing PCR.Results The activity level of lactate dehydrogenase in supernatants of the HL-7702 cells in isoniazid group(400, 800 mg/L)was higher than that in the control group(P<0.01).Compared with the control group,the mRNA expression of DNMT 1、3a、3b and GSTP1 were elevated in 400 and 800 mg/L isoniazid groups(P<0.05,P<0.01).The proteins expression of DNMT1 and 3a in the 400 and 800 mg/L isoniazid groups were higher than that in the control group(P<0.05,P<0.01),and the protein expression of DNMT 3b in the 800 mg/L isoniazid groups were higher than that in the control group(P<0.01).The methylation level of CpG is?land in GSTP1 promoter region of three isoniazid groups were decreased.Conclusion The CpG island in the promoter of GSTP1 has hypomethylation in hepatocyte cells damaged by isoniazid.
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The levels of Streptococcus (S.) mutans infections in saliva were evaluated and a comparison for specific antibody levels among children with different levels of S. mutans infection was made. The promising epitopic regions of antigen AgI/II (PAc) and glucosyltransferase (GTF) for potential vaccine targets related to S. mutans adherence were screened. A total of 94 children aged 3-4 years were randomly selected, including 53 caries-negative and 41 caries-positive children. The values of S. mutans and those of salivary total secretory immunoglobulin A (sIgA), anti-PAc and anti-Glucan binding domain (anti-GLU) were compared to determine the correlation among them. It was found the level of s-IgA against specific antigens did not increase with increasing severity of S. mutans infection, and the complete amino acid sequence of PAc and GTFB was analyzed using the DNAStar Protean system for developing specific anti-caries vaccines related to S. mutans adherence. A significantly positive correlation between the amount of S. mutans and children decayed, missing, and filled teeth index was observed. No significant difference was detected in specific sIgA against PAc or GLU between any two groups. No significant correlation was found between such specific sIgA and caries index. A total of 16 peptides from PAc as well as 13 peptides from GTFB were chosen for further investigation. S. mutans colonization contributed to early children caries as an important etiological factor. The level of sIgA against specific antigens did not increase with increasing severity of S. mutans infection in children. The epitopes of PAc and GTF have been screened to develop the peptide-based or protein-based anti-caries vaccines.
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Child, Preschool , Female , Humans , Male , Antibodies, Bacterial , Antigens, Bacterial , Chemistry , Allergy and Immunology , Bacterial Proteins , Chemistry , Allergy and Immunology , Case-Control Studies , Dental Caries , Allergy and Immunology , Pathology , Epitopes , Chemistry , Allergy and Immunology , Glucosyltransferases , Chemistry , Allergy and Immunology , Immunoglobulin A, Secretory , Peptides , Chemistry , Allergy and Immunology , Saliva , Chemistry , Microbiology , Severity of Illness Index , Streptococcal Vaccines , Chemistry , Allergy and Immunology , Streptococcus mutans , Chemistry , Allergy and Immunology , Virulence , Vaccines, Subunit , Virulence Factors , Chemistry , Allergy and ImmunologyABSTRACT
Objective: To clarify the cardioprotective effects of Yiqi Yangyin Huoxue (YYH) Injection exert against ischemia-reperfusion (I/R) injury, and the mechanisms involving activation of specific survival signals reperfusion injury salvage kinase (PI3K-Akt pathway and ERK1/2 pathway), hence inhibiting mitochondrial permeability transition pore opening (mPTP). Methods: A Langendorff model of myocardial ischemia-reperfusion injury was employed. The rats were randomly divided into seven groups: control group, I/R group, YYH 2.5, 5, and 10 μL/mL groups, LY294002 + YYH 10 μL/mL group, and LY294002 group. The cardiac parameters of left ventricular developed pressure (LVDP), maximal rise/fall of left ventricular pressure (± dp/dtmax), and rate-pressure product (RPP) were monitored. Creatine kinase (CK) and lactate dehydrogenase (LDH) released from effluents were measured. Infarct size was estimated by TTC staining. Transmission electron microscopy (TEM) was performed to assess morphological difference between cardiac mitochondrial isolated I/R rats and YYH pretreated rats. Western blotting was used to determine some protein expression. MPTP opening in mitochondria isolated from Langendorff rat hearts pretreated with YYH was measured. Incubation of isolated cardiac mitochondria with YYH and mPTP opening was measured. Results: YYH 10 μL/mL can significantly improve ventricular function, ameliorate the level of myocardial tissue lesions. YYH (5 and 10 μL/mL) reduced CK and LDH release. And these protections were accompanied by a significant increase in p-PKB/Akt, p-ERK1/2, and p-GSK-3β (Ser-9). LY294002 abolished the protective effects of YYH on cardiac function and infarct size and inhibited YYH-induced phosphorylation of PKB/Akt and GSK-3β. MPTP opening in mitochondria isolated from YYH-pretreated occurred more readily in vitro than I/R mitochondria. YYH (2.5, 5, 10, and 20 μL/mL), Shenmai injection (2.5, 5, 10, and 20 μL/mL) resulted in a significant inhibition of Ca2+-induced mitochondrial swelling. Conclusion: YYH produces direct cardioprotective actions. Inhibition of mPTP is a key event by which it mediates myocardial protection against I/R injury. And this effects involve activation of PI3K-Akt and ERK1/2 signal pathway. The mechanism of its action is different from that of CsA.
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<p><b>BACKGROUND</b>Urolithiasis in pediatric population is a serious problem with the incidence increased these years. In the management of larger stones (diameters >2 cm), percutaneous nephrolithotomy (PCNL) is considered to be the gold standard. This study aimed to investigate the efficacy and safety of mini-PCNL under total ultrasonography in patients aged <3 years.</p><p><b>METHODS</b>We reviewed 68 patients (80 renal units) aged <3 years between August 2006 and December 2014 in Peking University People's Hospital and Beijing Tsinghua Changung Hospital, including 36 renal units with a single stone, 6 with staghorn stones, 14 with upper ureteral stones, and 24 with multiple stones. The mean age of the patients was 24.2 months (range 6-36 months), and the mean maximum stone diameter was 19.2 mm (range 10-35 mm). The puncture site selection and tract dilation were guided by Doppler ultrasonography solely. All procedures were performed using 12-16 Fr tracts. Stones were fragmented using pneumatic lithotripsy and a holmium laser with an 8/9.8 Fr rigid ureteroscope.</p><p><b>RESULTS</b>Fifty-six patients with unilateral stones underwent a single session procedure, and 12 patients with bilateral stones underwent two procedures. The mean time to establish access was 2.8 min (range 1.8-5.0 min), the mean operative time was 36.5 min (range 20-88 min), the mean decrease in hemoglobin concentration was 8.9 g/L (2-15 g/L), and the stone-free rate (SFR) at hospital discharge was 94.0%. The mean postoperative hospital stay was 7.1 days (range 3-13 days). Postprocedure complications included fever (>38.5°C) in five patients and reactive pleural effusion in one patient. Blood loss requiring transfusion, sepsis, adjacent organ injury, and kidney loss were not observed.</p><p><b>CONCLUSIONS</b>This study indicated that ultrasound-guided mini-PCNL is feasible and safe in patients aged <3 years, without major complications or radiation exposure.</p>
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Child, Preschool , Female , Humans , Infant , Male , China , Kidney Calculi , Diagnostic Imaging , General Surgery , Nephrostomy, Percutaneous , Methods , Retrospective Studies , Treatment Outcome , Ultrasonography , Methods , Ureteral Calculi , Diagnostic Imaging , General SurgeryABSTRACT
This study examined the adhesive strength of two self-adhesive methacrylate resin-based sealers (MetaSEAL and RealSeal SE) to root dentin and compared them with RealSeal and AH Plus in properties. A total of 48 extracted human single-rooted teeth were used to prepare the 0.9-mm thick longitudinal tooth slice (each per tooth). Standardized simulated canal spaces of uniform dimensions were prepared in the middle of radicular dentin. After treated with 5.25% sodium hypochlorite (NaOCl) and 17% EDTA, tooth slices were allocated randomly to four groups (n=12) in terms of different sealers used: MetaSEAL, RealSeal SE, RealSeal, and AH plus groups. The simulated canal spaces were obturated with different sealers in each group. There were 10 slabs with 20 simulated canal spaces (n=20) used in each group for push-out testing. The failure modes and the ultrastructures of fractured sealer-dentin interfaces were examined. The remaining 2 slabs in each group underwent partial demineralization for observation of the ultrastructure of resin tags. The results showed that the push-out bond strength was 12.01±4.66 MPa in MetaSEAL group, significantly higher than that in the other three groups (P0.05). Mixed failures were predominant in the fractured sealer-dentin interfaces in MetaSEAL and AH Plus groups, while adhesive failures were frequently seen in RealSeal SE and RealSeal groups. In conclusion, after complete removal of the smear layer, MetaSEAL showed superior bond ability to root dentin. The RealSeal SE is applicable in clinical practice, with its adhesive strength similar to that of AH Plus. The self-adhesive methacrylate resin-based sealer holds promise for use in endodontic treatment.
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This study examined the adhesive strength of two self-adhesive methacrylate resin-based sealers (MetaSEAL and RealSeal SE) to root dentin and compared them with RealSeal and AH Plus in properties. A total of 48 extracted human single-rooted teeth were used to prepare the 0.9-mm thick longitudinal tooth slice (each per tooth). Standardized simulated canal spaces of uniform dimensions were prepared in the middle of radicular dentin. After treated with 5.25% sodium hypochlorite (NaOCl) and 17% EDTA, tooth slices were allocated randomly to four groups (n=12) in terms of different sealers used: MetaSEAL, RealSeal SE, RealSeal, and AH plus groups. The simulated canal spaces were obturated with different sealers in each group. There were 10 slabs with 20 simulated canal spaces (n=20) used in each group for push-out testing. The failure modes and the ultrastructures of fractured sealer-dentin interfaces were examined. The remaining 2 slabs in each group underwent partial demineralization for observation of the ultrastructure of resin tags. The results showed that the push-out bond strength was 12.01±4.66 MPa in MetaSEAL group, significantly higher than that in the other three groups (P<0.05). Moreover, no statistically significant differences were noted in the push-out bond strength between RealSeal SE (5.43±3.68 MPa) and AH Plus (7.34±2.83 MPa) groups and between RealSeal SE and RealSeal (2.93±1.76 MPa) groups (P>0.05). Mixed failures were predominant in the fractured sealer-dentin interfaces in MetaSEAL and AH Plus groups, while adhesive failures were frequently seen in RealSeal SE and RealSeal groups. In conclusion, after complete removal of the smear layer, MetaSEAL showed superior bond ability to root dentin. The RealSeal SE is applicable in clinical practice, with its adhesive strength similar to that of AH Plus. The self-adhesive methacrylate resin-based sealer holds promise for use in endodontic treatment.
Subject(s)
Humans , Adhesives , Reference Standards , Composite Resins , Reference Standards , Compressive Strength , Dental Bonding , Dental Pulp Cavity , Dental Stress Analysis , Methods , Dentin , Dentin-Bonding Agents , Reference Standards , Epoxy Resins , Reference Standards , Materials Testing , Methods , Methacrylates , Reference Standards , Microscopy, Electron, Scanning , Root Canal Filling Materials , Reference Standards , Root Canal Preparation , Tooth RootABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of high oxygen exposure on signaling pathway of the receptor for advanced glycation end products (RAGE)-NF-κB of lung in newborn rats and the mechanisms of protecting lung injury for human mesenchymal stem cells (hMSC).</p><p><b>METHODS</b>Twenty-four newborn Sprague-Dawley rats from three litters were randomly divided into three groups, as hyperoxia exposed + hMSC group (group A), hyperoxia exposed group (group B), and air-exposed group (group C). The rats from the group A and B were placed in a sealed Plexiglas chamber with a minimal in-and outflow, providing six to seven exchanges per hour of the chamber volume and maintaining O(2) levels above 95%, while rats in the group C only exposed to air simultaneously. Seven days later, rats in the group A were injected intravenously with hMSC (5×10(4)) after hyperoxia exposure, but rats in group B and C received subcutaneous injection with PBS alone at the same time point. Then all the rats were exposed to air, and were sacrificed three days later. Immunohistochemistry was used to evaluate the expression of RAGE in lung tissue. The levels of TNF-α and sRAGE in bronchoalveolar lavage fluid (BALF) and in serum were detected by ELASA, RAGE mRNA and NF-κB mRNA in tissue homogenates were detected by RT-PCR, RAGE and NF-κB by Western blotting; also the value of lung damage score were calculated with histology under light microscope.</p><p><b>RESULTS</b>There were significant differences among three groups in the fields of lung damage score (F = 51.59, P = 0.000), mRNA and protein of RAGE (F = 37.21, P = 0.000; F = 15.88, P = 0.000) and NF-κB (F = 5.695, P = 0.011; F = 4.223, P = 0.0288) in lung tissue homogenates, and the level of TNF-α (F = 38.29, P = 0.000) in BALF, all these parameters in group A and group B were higher than that in group C. While sRAGE in BALF in group A and group B were less than that in group C (F = 4.804, P = 0.0191). There were also significant differences between group A and group B in these parameters (P < 0.05). There were also no significant differences neither in TNF-α nor in sRAGE in serum among three groups.</p><p><b>CONCLUSIONS</b>hMSC protects hyperoxia-induced lung injury via downregulating the signaling pathway of RAGE-NF-κB.</p>
Subject(s)
Animals , Female , Humans , Male , Rats , Animals, Newborn , Disease Models, Animal , Glycation End Products, Advanced , Genetics , Metabolism , Hyperoxia , Metabolism , Lung , Metabolism , Pathology , Lung Injury , Metabolism , Mesenchymal Stem Cell Transplantation , NF-kappa B , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Random Allocation , Rats, Sprague-Dawley , Signal Transduction , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the expression of erythropoietin (EPO) and its receptor (EPOR) in the brain of newborn rats suffering fetal distress.</p><p><b>METHODS</b>A model of fetal distress was prepared by ligating bilateral uterine arteries of the rats with full-term pregnancy for 10 minutes before cesarean sections. The expression levels of EPO and EPOR in the brain of newborn rats were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot at 0, 2, 6, 12, 24, 48, 72 hrs and 7 days after birth. Serum EPO levels were measured using ELISA simultaneously. The newborn rats born by cesarean sections which were not subjected to uterine artery ligation were used as the control group.</p><p><b>RESULTS</b>The expression of EPO protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6 and 12 hrs after birth (P<0.05). The expression of EPOR protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6, 12, 24 and 48 hrs, and 3 days after birth (P<0.05). Serum EPO levels in the fetal distress group were significantly higher than in the control group 2 hrs after birth.</p><p><b>CONCLUSIONS</b>The EPO and EPOR levels in the brain increase quickly after birth in newborn rats suffering from fetal distress. The EPOR is high expressed for a longer time than EPO. This can provide a basis for the treatment of neonatal brain damage induced by fetal distress by exogenous EPO.</p>