ABSTRACT
Objective: To observe the effects of ginger-partitioned moxibustion at Shenque (CV8) and Guanyuan (CV4) on the expression levels of endocrine-related molecules and their receptors in rats with primary dysmenorrhea (PD) due to cold-dampness stagnation, thus to explore their analgesic mechanisms. Methods: Thirty-two female Wistar rats were divided into a normal group, a model group, a ginger-partitioned moxibustion group, and a Western medicine group according to the random number table method, with 8 rats in each group. Except for rats in the normal group, all other rats were treated with oxytocin combined with ice-water bath to establish the rat models of PD due to cold-dampness stagnation. After successful modeling, rats in the normal group and the model group did not receive treatment; rats in the ginger-partitioned moxibustion group received treatments with ginger-partitioned moxibustion at Shenque (CV8) and Guanyuan (CV4); rats in the Western medicine group received ibuprofen by intragastric administration. The writhing response of rats was compared among groups, and the serum levels of prostaglandin F2α (PGF2α), estrogen (estradiol, E2), progesterone (P), and the mRNA expression of PGF2α and E2 receptors in the uterine tissues were detected. Results: No writhing behavior was observed in the normal group; compared with the normal group, the serum PGF2α and E2 levels in the model group were increased (P<0.01), while the P level was decreased (P<0.01), and the mRNA expression levels of the uterine PGF2α and E2 receptors were increased (P<0.01, P<0.05). Compared with the model group, the writhing behavior latency was prolonged, and the writhing response score was decreased in the ginger-partitioned moxibustion group and the Western medicine group (P<0.01); the serum PGF2α and E2 levels in the ginger-partitioned moxibustion group and the Western medicine group were decreased, while the P level was increased (P<0.05 or P<0.01); the mRNA expression levels of the uterine PGF2α and E2 receptors in the ginger-partitioned moxibustion group and the Western medicine group were decreased (P<0.05). Compared with the Western medicine group, the ginger-partitioned moxibustion group showed a prolonged writhing behavior latency, reduced writhing response score (P<0.05), and decreased serum E2 level (P<0.05), while no statistical differences in the serum PGF2α and P levels, or the mRNA expression levels of uterine PGF2α and E2 receptors (P>0.05).Conclusion: The analgesic effect of ginger-partitioned moxibustion on PD due to cold-dampness stagnation may be related to regulating the mRNA expression levels of PGF2α and E2 receptors in the uterine tissues.
ABSTRACT
Objective To observe the clinical curative effect of patients with ovulation obstructive infertility by combined treatmentofstimulating ovulation particles and clmiphene citrateclinical. Methods 171 patients were randomly divided into Traditional Chinese medicine (TCM) group (42 cases), clmiphene citrateclinical (CC) group (53 cases) andcombined treatment of TCM and CC group (76 cases). Ultrasound examinations were used to monitor the development of endometrium, follicular development and value of Plusatility Index (PI). The levels of E2,LH and P were measured before and after medication. And pregnancy of patients was followed up. Results Ovulation rates of combined treatment of TCM and CC group and the CC group were both higher than TCM group (P<0.05). Pregnancy rate of combined treatment of TCM and CC group was higher than TCM group and CC group (P<0.05). Endometrial thickness and type A lining rate of combined treatment of TCM and CC groupandTCM group were both higher than CC group (P < 0.05). The values of PI of combined treatment of TCM and CC groupand TCMgroup were both lower than CC group on HCG day after treatment (P<0.05). E2 values ofpost-treatment of three groups were all significantly higher than that before treatment.Difference in values of E2 and LH between pretherapy and post-treatment of combined treatment of TCM and CCwere obviously higher than that of TCM group (P<0.05).Difference in values of P between pretherapy and post-treatment of tcombined treatment of TCM and CC and TCM group were higher than CC group on the seventh day after ovulation (P < 0.05). Conclusions Combination of traditional Chinese medicine and CCcouldnot only promote follicle growth , but also improve anti-estrogen role of CC , endometrial blood flow and endocrine regulation , enhance ovulation rate and decrease the rate of abortion. Ovulation obstructive infertility treatment was better than that of CC and TCM treatment.